BREEDIT: Fast breeding tools to match the fast pace of climate change

Osnato, Michela

doi:10.1093/plcell/koac328

Cited by 2 publications

(1 citation statement)

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“…Plant breeding includes generating genetic variants and selecting favourable plant traits via traditional and modern techniques (Osnato, 2023 ). To achieve novel and usable traits, the plant's genetic codes must be modified via spontaneous or introduced mutations by natural incidence or artificial means (Breseghello and Coelho, 2013 ; Gao, 2021 ; Lorenzo et al ., 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Prime editing: Mechanism insight and recent applications in plants

Vu,

Nguyen,

Kim

et al. 2023

Plant Biotechnology Journal

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SummaryPrime editing (PE) technology utilizes an extended prime editing guide RNA (pegRNA) to direct a fusion peptide consisting of nCas9 (H840) and reverse transcriptase (RT) to a specific location in the genome. This enables the installation of base changes at the targeted site using the extended portion of the pegRNA through RT activity. The resulting product of the RT reaction forms a 3′ flap, which can be incorporated into the genomic site through a series of biochemical steps involving DNA repair and synthesis pathways. PE has demonstrated its effectiveness in achieving almost all forms of precise gene editing, such as base conversions (all types), DNA sequence insertions and deletions, chromosomal translocation and inversion and long DNA sequence insertion at safe harbour sites within the genome. In plant science, PE could serve as a groundbreaking tool for precise gene editing, allowing the creation of desired alleles to improve crop varieties. Nevertheless, its application has encountered limitations due to efficiency constraints, particularly in dicotyledonous plants. In this review, we discuss the step‐by‐step mechanism of PE, shedding light on the critical aspects of each step while suggesting possible solutions to enhance its efficiency. Additionally, we present an overview of recent advancements and future perspectives in PE research specifically focused on plants, examining the key technical considerations of its applications.

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