Brevibacillus laterosporus as perspective source of new bioinsecticides

Ghazanchyan, N.L.; Kinosyan, Marina; Tadevosyan, P.E.; Khachaturyan, N.S.; Afrikian, E. G.

doi:10.1016/j.aasci.2018.03.011

Cited by 9 publications

(5 citation statements)

References 7 publications

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“…Brevibacillus laterosporus has long been noted for its broad-spectrum antifungal properties when isolated from rhizosphere soil samples 22 , which has been associated with the production of a wide range of enzymes and antibiotics. Ghazanchyan et al, (2018) reported that Brevibacillus laterosporus is reported to strongly inhibit the growth of numerous apple phytopathogens (such as Rhizoctonia solani , Fusarium oxysporum , Fusarium solani , and Physalospora piricola ) and suppress potato common scab by reducing pathogen abundance and regulating soil bacterial community 23 , 24 . Bacillus mucilaginosus , known as potassium bacteria or silicate bacteria, is an important functional bacteria widely used in microbial fertilizers 25 .…”

Section: Discussionmentioning

confidence: 99%

Approach for quick exploration of highly effective broad-spectrum biocontrol strains based on PO8 protein inhibition

Gu,

Fu,

Yan

et al. 2023

npj Sci Food

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Aflatoxin is a group of strongly toxic and carcinogenic mycotoxins produced by Aspergillus flavus and other Aspergillus species, which caused food contamination and food loss problems widely across the world especially in developing countries, thus threatening human health and sustainable development. So, it is important to develop new, green, and broad-spectrum biocontrol technology for the prevention of aflatoxin contamination sources. Previously, we found that the PO8 protein from aflatoxigenic A. flavus could be used as a biomarker to predict aflatoxin production in peanuts (so the PO8 is named as an early warning molecule), which infers that the PO8 is relative to aflatoxin production. Therefore, in the study, based on inhibiting the PO8, a new and quick strategy for screening aflatoxin biocontrol strains for developing control agents was presented. With the PO8 inhibition method, four biocontrol strains (2 strains were isolated from peanut kernels with sterilized surface and another 2 strains from peanut rhizosphere soil) were selected and combined to increase prevention wide-spectrum. As a result, the combination showed over 90% inhibition to all tested aflatoxigenic A. flavus isolated from three different peanut production areas (north, middle, and south areas of China), and better than any single strain. The field experiments located in five provinces of China showed that the practice prevention effects (inhibition of aflatoxigenic fungi on the surface of the peanuts) were from 50% to over 80%. The results indicated that the strategy of inhibiting the early warning molecule PO8 can be used to develop aflatoxin control agents well.

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Section: Discussionmentioning

confidence: 99%

Approach for quick exploration of highly effective broad-spectrum biocontrol strains based on PO8 protein inhibition

Gu,

Fu,

Yan

et al. 2023

npj Sci Food

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“…Saikia et al 42 . were the first to find that an antagonistic bacterium – B. laterosporus strain BPM3 – was able to control blast disease of rice, while Ghazanchyan et al 43 . noted insecticidal activity of B. laterosporus strains against leaf beetles.…”

Section: Discussionmentioning

confidence: 99%

“…Brevibacillus laterosporus is particularly known for being either a biocontrolling agent for plant pathogens or a bioinsecticide. Saikia et al 42 were the first to find that an antagonistic bacterium -B. laterosporus strain BPM3was able to control blast disease of rice, while Ghazanchyan et al 43 noted insecticidal activity of B. laterosporus strains against leaf beetles. In the present study, B. lateropsorus K75 significantly increased the growth of maize.…”

Section: Discussionmentioning

confidence: 99%

Physiological properties and genomic insights into the plant growth‐promoting rhizobacterium Brevibacillus laterosporus K75 isolated from maize rhizosphere

et al. 2022

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BACKGROUND When looking for a safer alternative to pesticides that are potentially harmful to living organisms, one of the directions worth looking at are plant growth‐promoting rhizobacteria. The purpose of the research was a comprehensive characterization of Brevibacillus laterosporus K75, a strain isolated from maize rhizosphere. Many studies have proved B. laterosporus to be a biocontrol agent; however, little is known about B. laterosporus as a plant growth‐promoting rhizobacterium. RESULTS Ninety strains were screened for plant growth‐promoting activities. Four strains with the best plant growth‐promoting traits (Rhodococcus qingshengii K8, Bacillus subtilis subsp. stercoris K73, Brevibacillus laterosporus K75, and Brevibacillus laterosporus K89) were used to research their effect on maize growth. Under sterile conditions, B. laterosporus K75 showed the best stimulatory effect, significantly improving the weight of roots, shoots and leaves, and considerably increasing content of chlorophyll. In unsterilized soil, B. laterosporus K75 significantly improved length of roots and weight of leaves compared to the K73, K89, and untreated control. Moreover, B. laterosporus K75 significantly increased specific leaf area compared to the untreated control and to other inoculant treatments. The genome of B. laterosporus K75 was compared to the recently published B. laterosporus MG64. Genome‐mining displayed differences in identified plant growth‐promoting genes and biosynthetic gene clusters of secondary metabolites. The B. laterosporus K75 genome possessed additional genes involved in indole‐3‐acetic acid production and phosphate solubilization that could be attributed to its ability to enhance maize growth. CONCLUSION Our study demonstrated that B. laterosporus K75 is a promising candidate for use in inoculant formulation, effectively facilitating maize growth. © 2022 Society of Chemical Industry.

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“…In recent years, studies on B. laterosporus have increased and confirmed that the different strains have a number of valuable properties in terms of applied microbiology (Ghazanchyan, Kinosyan, Tadevosyan, Khachaturyan, & Afrikian, 2018), such as the abundance of antibiotics and important sources of antibacterial substances, acting in the treatment of diseases and even in food preservation (Demirkan, Aybey, & Ak, 2021;Ruiu, 2013). B. laterosporus spores have also been reported as a probiotic for birds and mammals, when administered as food, as it improves feed conversion and weight gain (Meghwal, Goyal, & Chavan, 2017;Purba et al, 2020;Wolfenden et al, 2011) Curculionidae) (Oliveira et al, 2004), Lasioderma serricorne (Coleoptera: Anobiidae), and Simulium vittatum (Diptera: Simuliidae) (Singer, 1996).…”

Section: Discussionmentioning

confidence: 99%

Ultrastructural and pathogenicity of Brevibacillus laterosporus against sinantropic muscoid dipterans

Pereira¹,

Queiroz

Faria

et al. 2021

Microscopy Res & Technique

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Brevibacillus laterosporus has entomopathogenic potential against several orders of insects and its wide bioactivity is associated with a variety of strain-specific molecules.In order to avoid the use of synthetic insecticides, along with the need to control insect pests, microbial control has been widely used. Muscoid dipterans are known for their medical-veterinary and sanitary importance, and synanthropy. The enormous biotechnological potential of B. laterosporus has been demonstrated, but there are still few studies with muscoid dipterans. The aim of the study was to verify the mortality of B. laterosporus NRS590 on synanthropic flies and to characterize its different cell stages ultrastructurally. The flies were collected from garbage bins and the colonies were adapted to the laboratory conditions. Bioassays with neo larvae were carried out from the bacterial growth in the phases: vegetative (6 hr), sporangium (20 hr), and free spores (44 hr). An aliquot of each phase was collected for Transmission (TEM) and Scanning Electron Microscopy (SEM). The effectiveness of NRS590 was observed in the sporulation phase, where the corrected mortality was 83.3, 85.1, and 99% for Chrysomya megacephala, Chrysomya putoria, and Musca domestica, respectively. The parasporal body was observed in detail on the entire spore surface. Although our knowledge of this bacterium is growing, it remains to be determined the real virulence factors responsible for the wide entomopathogenic activity observed on muscoid dipterans. Therefore, this study can provide subsidies for the improvement of efficient and safe microbial control techniques for the environment and living beings.

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Brevibacillus laterosporus as perspective source of new bioinsecticides

Cited by 9 publications

References 7 publications

Approach for quick exploration of highly effective broad-spectrum biocontrol strains based on PO8 protein inhibition

Approach for quick exploration of highly effective broad-spectrum biocontrol strains based on PO8 protein inhibition

Physiological properties and genomic insights into the plant growth‐promoting rhizobacterium Brevibacillus laterosporus K75 isolated from maize rhizosphere

Ultrastructural and pathogenicity of Brevibacillus laterosporus against sinantropic muscoid dipterans

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