BRF Negatively Regulates Thermotolerance Defect of fes1a in Arabidopsis

Fu, Can; Liu, Xiaxia; Li, Xuezhi; Huo, Panfei; Ge, Jingjing; Hou, Yanfei; Yang, Wenwen; Zhang, Jingxia; Zhang, Limin; Zhao, Dazun; Ma, Changle; Liu, Jian

doi:10.3389/fpls.2020.00171

Cited by 5 publications

(5 citation statements)

References 32 publications

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“…DEL2 altered expression caused changes in cell division and elongation in the root meristem and affected the expression of several cell-cycle regulators [57]. Moreover, the BRF3 gene, which is part of the core unit of the RNA Pol III transcription complex [58], was shown to negatively regulates the thermotolerance in Arabidopsis [59]. The up-regulation of these genes in ros1-4 during germination could be part of the answer for the decrease in ros1-4 seed germination upon heat stress.…”

Section: Resultsmentioning

confidence: 99%

Regulation of DNA (de)methylation positively impacts seed germination during seed development under heat stress

Malabarba

Windels

et al. 2021

Preprint

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Seed development needs the coordination of multiple molecular mechanisms to promote correct tissue development, seed filling and the acquisition of germination capacity, desiccation tolerance, longevity and dormancy. Heat stress can negatively impact these processes and upon the increasing of global mean temperatures, global food security is threatened. Here, we explored the impact of heat stress on seed physiology, morphology, gene expression and methylation on three stages of seed development. Notably, Arabidopsis Col-0 plants under heat stress presented a decrease in germination capacity and also a decrease in longevity. We observed that upon mild stress, gene expression and DNA methylation were moderately affected. Nevertheless, upon severe heat stress during seed development, gene expression was intensively modified, promoting heat stress response mechanisms, including the activation of ABA pathway. By analyzing candidate epigenetic marks using mutants physiological assays, we observed that the lack of DNA demethylation by ROS1 gene impaired seed germination by affecting germination-related genes expression. On the other hand, we also observed that upon severe stress, a large proportion of differentially methylated regions (DMRs) were located in promoters and gene sequences of germination-related genes. To conclude, our results indicate that DNA (de)methylation could be a key regulatory process to ensure proper seed germination of seeds produced under heat stress.

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Section: Resultsmentioning

confidence: 99%

Regulation of DNA (de)methylation positively impacts seed germination during seed development under heat stress

Malabarba

Windels

et al. 2021

Preprint

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“…In Arabidopsis, three Fes1s were identified and attention has been paid to AtFes1A . Loss of AtFes1A resulted in thermotolerance defect, while the single knockout of a TFIIIB-related factor gene or a Bcl2-associated athanogene domain gene could suppress this defect ( Zhang et al., 2010 ; Fu et al., 2015 ; Fu et al., 2019 ; Fu et al., 2020 ). The roles of Fes1C in ER and salt stress were also characterized in rice ( Qian et al., 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Distribution, expression of hexaploid wheat Fes1s and functional characterization of two TaFes1As in Arabidopsis

et al. 2022

Front. Plant Sci.

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Hexaploid wheat is a major food crop and is sensitive to heat stress. It is necessary to discover genes related to thermotolerance in wheat. Fes1s is a class of nucleotide exchange factor of heat shock protein 70s, proven to be participated in heat response in human, yeast, and Arabidopsis. However, little is known about Fes1s in hexaploid wheat. In this study, we identified nine Fes1s in hexaploid wheat (TaFes1s) and found that they present as three triads. A phylogenetic relationship analysis revealed that these Fes1s grouped into Fes1A, Fes1B and Fes1C subclades, and Fes1As and Fes1Bs were divergent in monocots, but possibly not in dicots. The sequences, gene structures and protein motifs of TaFes1s homoeologues within a triad were highly conserved. Through cis-elements analysis including heat shock elements, and miRNA targets prediction, we found that regulation of three TaFes1s homoeologues may be different, while the expression patterns of three homoeologues were similar. The expression levels of TaFes1As were higher than those of TaFes1Bs and TaFes1Cs, and based on these expressions, TaFes1As were chosen for functional characterization. Intriguingly, neither TaFes1A-5A nor TaFes1A-5D could not rescue the thermotolerance defect of Arabidopsis fes1a mutants at seedling stage, but in the transgenic plants seed germination was accelerated under normal and heat stress condition. The functional characterization indicated that roles of Fes1As would be different in Arabidopsis and hexaploid wheat, and function retention of TaFes1As may occur during wheat evolution. In conclusion, our study comprehensively characterized the distribution and expression of Fes1s in hexaploid wheat and found that two TaFes1As could accelerate seed germination under normal and heat stress condition.

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“…As cell elongation is the first step in the germination stage, before cell division [ 59 , 60 ], DEL2 might repress radicle emergence. Moreover, the BRF3 gene, which is part of the core unit of the RNA Pol III transcription complex [ 61 ], was shown to negatively regulate the thermotolerance in Arabidopsis [ 62 ]. The upregulation of these genes in ros1-4 during germination could be part of the answer for the decrease in ros1-4 seed germination upon heat stress.…”

Section: Whole-genome Dna Methylation Of Seeds Under Heat Stressmentioning

confidence: 99%

Regulation of DNA (de)Methylation Positively Impacts Seed Germination during Seed Development under Heat Stress

Malabarba

Windels

et al. 2021

Genes

View full text Add to dashboard Cite

Seed development needs the coordination of multiple molecular mechanisms to promote correct tissue development, seed filling, and the acquisition of germination capacity, desiccation tolerance, longevity, and dormancy. Heat stress can negatively impact these processes and upon the increase of global mean temperatures, global food security is threatened. Here, we explored the impact of heat stress on seed physiology, morphology, gene expression, and methylation on three stages of seed development. Notably, Arabidopsis Col-0 plants under heat stress presented a decrease in germination capacity as well as a decrease in longevity. We observed that upon mild stress, gene expression and DNA methylation were moderately affected. Nevertheless, upon severe heat stress during seed development, gene expression was intensively modified, promoting heat stress response mechanisms including the activation of the ABA pathway. By analyzing candidate epigenetic markers using the mutants’ physiological assays, we observed that the lack of DNA demethylation by the ROS1 gene impaired seed germination by affecting germination-related gene expression. On the other hand, we also observed that upon severe stress, a large proportion of differentially methylated regions (DMRs) were located in the promoters and gene sequences of germination-related genes. To conclude, our results indicate that DNA (de)methylation could be a key regulatory process to ensure proper seed germination of seeds produced under heat stress.

show abstract

BRF Negatively Regulates Thermotolerance Defect of fes1a in Arabidopsis

Cited by 5 publications

References 32 publications

Regulation of DNA (de)methylation positively impacts seed germination during seed development under heat stress

Regulation of DNA (de)methylation positively impacts seed germination during seed development under heat stress

Distribution, expression of hexaploid wheat Fes1s and functional characterization of two TaFes1As in Arabidopsis

Regulation of DNA (de)Methylation Positively Impacts Seed Germination during Seed Development under Heat Stress

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