BrFLC5: a weak regulator of flowering time in Brassica rapa

Xi, Xi; Wei, Keyun; Gao, Baozhen; Liu, Jiahe; Liang, Jiansheng; Cheng, Feng; Wang, Xiaowu; Wu, Jian

doi:10.1007/s00122-018-3139-x

Cited by 32 publications

(38 citation statements)

References 29 publications

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“…The common AP1 down-regulation in floral meristems is commonly associated with late flowering [27]. Moreover, FLC a central player in the vernalization pathway consists of four copies in the B. rapa genome [28]. Unlike, FLC1, FLC3, and FLC2 which are known to delay flowering in B. rapa, FLC5 is a relatively weak regulator in B. rapa.…”

Section: Degs Changes In Multiple Signal Transduction Pathway Affect mentioning

confidence: 99%

Comparative transcriptome analysis revealed differential gene expression in multiple signaling pathways at flowering in polyploid Brassica rapa

et al. 2021

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Background Polyploidy is widespread in angiosperms and has a significant impact on plant evolution, diversity, and breeding program. However, the changes in the flower development regulatory mechanism in autotetraploid plants remains relatively limited. In this study, RNA-seq analysis was used to investigate changes in signaling pathways at flowering in autotetraploid Brassica rapa. Results The study findings showed that the key genes such as CO, CRY2, and FT which promotes floral formation were down-regulated, whereas floral transition genes FPF1 and FD were up-regulated in autotetraploid B. rapa. The data also demonstrated that the positive regulators GA1 and ELA1 in the gibberellin’s biosynthesis pathway were negatively regulated by polyploidy in B. rapa. Furthermore, transcriptional factors (TFs) associated with flower development were significantly differentially expressed including the up-regulated CIB1 and AGL18, and the down-regulated AGL15 genes, and by working together such genes affected the expression of the down-stream flowering regulator FLOWERING LOCUS T in polyploid B. rapa. Compared with that in diploids autotetrapoid plants consist of differential expression within the signaling transduction pathway, with 13 TIFY gens up-regulated and 17 genes related to auxin pathway down-regulated. Conclusion Therefore, polyploidy is more likely to integrate multiple signaling pathways to influence flowering in B. rapa after polyploidization. In general, the present results shed new light on our global understanding of flowering regulation in polyploid plants during breeding program.

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Section: Degs Changes In Multiple Signal Transduction Pathway Affect mentioning

confidence: 99%

Comparative transcriptome analysis revealed differential gene expression in multiple signaling pathways at flowering in polyploid Brassica rapa

et al. 2021

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“…The primer mix and PCRs were programmed as recommended by LGC. The KASP thermal cycling conditions were those described in Xi et al 47 . Fluorescence was detected with a QuantStudio 6 instrument (Applied Biosystems, Foster City, CA, USA).…”

Section: Snp Genotype By Kasp (Kompetitive Allele-specific Pcr) Assaymentioning

confidence: 99%

Defect in Brnym1, a magnesium-dechelatase protein, causes a stay-green phenotype in an EMS-mutagenized Chinese cabbage (Brassica campestris L. ssp. pekinensis) line

et al. 2020

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Leaf color is an important target trait in Chinese cabbage breeding programs. Leaf yellowing may reduce crop commercial and nutritional values. Some plants with the "stay-green" trait maintain leaf greenness during senescence and even after death. Stay-green Chinese cabbage may be a focal point of future breeding projects because it could improve crop quality and yield and prolong shelf life. A new stay-green mutant, non-yellowing mutant 1 (nym1), was identified in Chinese cabbage derived from an ethyl methane sulfonate (EMS)-mutagenized population. The mutant had stay-green characteristics and a higher chlorophyll content than the wild-type during leaf senescence. The staygreen trait in the mutant Chinese cabbage was controlled by the recessive gene Brnym1. MutMap and KASP analyses showed that Brnym1 (BraA03g050600.3C) encodes an mg-dechelatase (SGR protein), which might be the causal gene of the mutation in Chinese cabbage. A nonsynonymous single nucleotide base substitution (G to A) in the third exon of Brnym1 caused an amino acid substitution from L to F in the highly conserved domain of the magnesiumdechelatase. Ectopic overexpression showed that the BrNYM1 gene of wild-type Chinese cabbage complemented the SGR-defective stay-green mutant nye1-1 of Arabidopsis. The magnesium-dechelatase activity in the nym1 mutant was significantly downregulated compared to that in the wild type. Brnym1 was relatively upregulated in the mutant during late senescence, and BrNYM1 was localized to the chloroplasts. These results indicate that Brnym1 (BraA03g050600.3C) is the causal gene of the stay-green mutation and could be of particular significance in the genetic improvement of Chinese cabbage.

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“…A recent study showed that the reference genome sequence indeed contains a truncated BrFLC5 sequence, while other accessions contain functional genes with different splicing patterns resulting from a single nucleotide mutation. Genetic variation within the BrFLC5 locus indicates that BrFLC5 is not a major regulator of flowering time [96]. BrFLC2 acts as a repressor of flowering when overexpressed in A. thaliana and shows early flowering when silenced in B. rapa ssp.…”

Section: Conserved and Divergent Flowering Time Genes In Brassicacmentioning

confidence: 99%

Translating Flowering Time from Arabidopsis thaliana to Brassicaceae and Asteraceae Crop Species

Leijten

Koes

Roobeek

et al. 2018

Plants

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Flowering and seed set are essential for plant species to survive, hence plants need to adapt to highly variable environments to flower in the most favorable conditions. Endogenous cues such as plant age and hormones coordinate with the environmental cues like temperature and day length to determine optimal time for the transition from vegetative to reproductive growth. In a breeding context, controlling flowering time would help to speed up the production of new hybrids and produce high yield throughout the year. The flowering time genetic network is extensively studied in the plant model species Arabidopsis thaliana, however this knowledge is still limited in most crops. This article reviews evidence of conservation and divergence of flowering time regulation in A. thaliana with its related crop species in the Brassicaceae and with more distant vegetable crops within the Asteraceae family. Despite the overall conservation of most flowering time pathways in these families, many genes controlling this trait remain elusive, and the function of most Arabidopsis homologs in these crops are yet to be determined. However, the knowledge gathered so far in both model and crop species can be already exploited in vegetable crop breeding for flowering time control.

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BrFLC5: a weak regulator of flowering time in Brassica rapa

Cited by 32 publications

References 29 publications

Comparative transcriptome analysis revealed differential gene expression in multiple signaling pathways at flowering in polyploid Brassica rapa

Comparative transcriptome analysis revealed differential gene expression in multiple signaling pathways at flowering in polyploid Brassica rapa

Defect in Brnym1, a magnesium-dechelatase protein, causes a stay-green phenotype in an EMS-mutagenized Chinese cabbage (Brassica campestris L. ssp. pekinensis) line

Translating Flowering Time from Arabidopsis thaliana to Brassicaceae and Asteraceae Crop Species

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