2020
DOI: 10.3389/fcell.2020.00774
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BRG1 Activates PR65A Transcription to Regulate NO Bioavailability in Vascular Endothelial Cells

Abstract: Vascular endothelial cells contribute to the pathogenesis of cardiovascular diseases by producing and disseminating angiocrine factors. Nitric oxide (NO), catalyzed by endothelial NO synthase (eNOS), is one of the prototypical angiocrine factors. eNOS activity is modulated by site-specific phosphorylation. We have previously shown that endothelial-specific knockdown of BRG1 in Apoe –/– mice attenuates the development of atherosclerosis, in which eNOS-dependent NO catalysis p… Show more

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Cited by 24 publications
(18 citation statements)
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“…Transient transfections were performed with Lipofectamine 2000 (Invitrogen). Luciferase activities were assayed 24–48 h after transfection using a luciferase reporter assay system (Promega) as previously described ( Chen et al, 2020a , c ; Li et al, 2020a ).…”
Section: Methodsmentioning
confidence: 99%
“…Transient transfections were performed with Lipofectamine 2000 (Invitrogen). Luciferase activities were assayed 24–48 h after transfection using a luciferase reporter assay system (Promega) as previously described ( Chen et al, 2020a , c ; Li et al, 2020a ).…”
Section: Methodsmentioning
confidence: 99%
“…Primary hepatocytes were isolated and cultured as previously described ( Fan et al, 2020 ). Mouse Npnt promoter-luciferase constructs ( Lanthier et al, 2011 ) and BRG1 expression constructs have been previously described ( Chen et al, 2020c ; Li et al, 2020a,b , c ). Small interfering RNAs were purchased from Dharmacon.…”
Section: Methodsmentioning
confidence: 99%
“…Chromatin immunoprecipitation (ChIP) assays were performed essentially as described before ( Chen et al, 2020a , b , c ; Dong et al, 2020 ; Fan et al, 2020 ; Li et al, 2020a , b , c ; Lv et al, 2020 ; Mao et al, 2020 ; Sun et al, 2020 ; Wu et al, 2020 ; Yang et al, 2020 ). In brief, chromatin in control and treated cells were cross-linked with 1% formaldehyde.…”
Section: Methodsmentioning
confidence: 99%