Bridging the Gap: Plant‐Endophyte Interactions as a Roadmap to Understanding Small‐Molecule Communication in Marine Microbiomes

Samples, Robert M.; Balunas, Marcy J.

doi:10.1002/cbic.202000064

Cited by 2 publications

(2 citation statements)

References 132 publications

(187 reference statements)

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“…This inferred that maintenance of a highly reducing cytoplasm could be an important protective strategy of actinobacterial strains against the hostile environment of mangrove plants. Of note, some plants might also uptake extracellular ergothioneine as an additional antioxidant through symbiotic relationship with beneficial microbes (Samples and Balunas 2020). In support of the genomic findings, strain VCCM 22513 was notably more resistant to H 2 O 2 -induced oxidative stress as compared to endophytic S. cavourensis YBQ59.…”

Section: Discussionmentioning

confidence: 53%

Genomic and physiological traits provide insights into ecological niche adaptations of mangrove endophytic Streptomyces parvulus VCCM 22513

Quach

Bui

et al. 2022

Ann Microbiol

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Purpose Endophytic Streptomyces parvulus VCCM 22513 isolated from Bruguiera gymnorrhiza in Quang Ninh mangrove forest, northern Vietnam showed abiotic stress tolerance consisting of antioxidant, salt-tolerant, and aromatic-compound degrading activities. The goal of this study was to shed light on genomic bases rendering mangrove endophytic S. parvulus more resilient to environmental stressors. Methods Phenotypic analysis including antioxidant activities, hydrogen peroxide and sodium chloride resistance, and aromatic compound utilization were evaluated. The genome of strain VCCM 22513 was sequenced using Illumina Miseq sequencing platform and assembled using SPAdes. Results Out of 15 endophytic actinomycetes associated with B. gymnorrhiza in Quang Ninh mangrove, northern Vietnam, VCCM 22513 extract showed remarkable antioxidant activities through (1,1-diphenyl-2-picrylhydrazyl) DPPH and superoxide radical scavenging assays of 72.1 ± 0.04% and 38.3 ± 0.16% at 1.6 mg/ml, respectively. The genome consists of a 7,688,855 bp linear chromosome, 6782 protein-coding sequences, and 68 tRNAs. Genomic analysis identified strain VCCM 22513 as Streptomyces parvulus and confirmed a highly conserved core genome and stability of S. parvulus under natural selection. Genome mining revealed the presence of genetic determinants involved in mycothiol and ergothioneine biosynthesis (26 genes), oxidative stress resistance (43 genes), osmoadaptation (87 genes), heat and cold stress (34 genes), aromatic compound degradation (55 genes). Further genome-wide comparison between S. parvulus VCCM 22513 and 11 Streptomyces genomes showed that VCCM 22513 possesses significantly higher copies of genes involved in mycothiol and ergothioneine biosynthesis. In support of this finding, the strain exhibited much resistance to 0.6–1.0 M H2O2 and 6% (w/v) NaCl as compared to Streptomyces cavourensis YBQ59 isolated from Cinnamomum cassia Prels. In addition, the complete pathways for degradation of aromatic compounds including protocatechuate, gentisate, 4-hydroxyphenylpyruvate, cinnamate, 3-phenylpropionate, and styrene were only identified in the genome of VCCM 22513. Conclusions The present study revealed for the first time adaptive responses of mangrove endophytic S. parvulus VCCM 22513 to survive in hostile environment. The information shown here provided better understanding of underlying mechanisms related to adaptation and partially plant-microbe interaction of Streptomyces associated with mangrove plants.

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Section: Discussionmentioning

confidence: 53%

Genomic and physiological traits provide insights into ecological niche adaptations of mangrove endophytic Streptomyces parvulus VCCM 22513

Quach

Bui

et al. 2022

Ann Microbiol

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“…In addition, chemical ecology studies of eukaryotic–prokaryotic or prokaryotic–prokaryotic interactions could provide insights into possible novel molecular targets of ecologically active compounds relevant to treating human ailments such as microbial infectious. Furthermore, chemical ecology investigations of host–symbiont symbioses, particularly in marine holobionts, can provide an understanding of the roles of microflora/microbiome in human health [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Impact of Marine Chemical Ecology Research on the Discovery and Development of New Pharmaceuticals

Tan

2023

Marine Drugs

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Diverse ecologically important metabolites, such as allelochemicals, infochemicals and volatile organic chemicals, are involved in marine organismal interactions. Chemically mediated interactions between intra- and interspecific organisms can have a significant impact on community organization, population structure and ecosystem functioning. Advances in analytical techniques, microscopy and genomics are providing insights on the chemistry and functional roles of the metabolites involved in such interactions. This review highlights the targeted translational value of several marine chemical ecology-driven research studies and their impact on the sustainable discovery of novel therapeutic agents. These chemical ecology-based approaches include activated defense, allelochemicals arising from organismal interactions, spatio-temporal variations of allelochemicals and phylogeny-based approaches. In addition, innovative analytical techniques used in the mapping of surface metabolites as well as in metabolite translocation within marine holobionts are summarized. Chemical information related to the maintenance of the marine symbioses and biosyntheses of specialized compounds can be harnessed for biomedical applications, particularly in microbial fermentation and compound production. Furthermore, the impact of climate change on the chemical ecology of marine organisms—especially on the production, functionality and perception of allelochemicals—and its implications on drug discovery efforts will be presented.

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Bridging the Gap: Plant‐Endophyte Interactions as a Roadmap to Understanding Small‐Molecule Communication in Marine Microbiomes

Abstract: This article is part of a Special Collection on Microbial Biosynthesis and Interactions. To view the complete collection, visit our homepage

Cited by 2 publications

References 132 publications

Genomic and physiological traits provide insights into ecological niche adaptations of mangrove endophytic Streptomyces parvulus VCCM 22513

Genomic and physiological traits provide insights into ecological niche adaptations of mangrove endophytic Streptomyces parvulus VCCM 22513

Impact of Marine Chemical Ecology Research on the Discovery and Development of New Pharmaceuticals

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