Brief communication. In situ hybridization mapping of genes in Hordeum vulgare L.

Genetic induction of structural changes in barley chromosomes added to common wheat by a gametocidal chromosome derived from Aegilop Scylindrica.

“…However, cytogenetic stocks such as aneuploids and deletion lines are either very difficult or impossible to maintain in barley as it is a diploid. To date, various techniques have been used to conduct physical mapping in barley, such as PCR mediated localization of DNA sequences on microisolated translocation chromosomes (Sorokin et al 1994), pulsed field gel electrophoresis (Siedler and Graner, 1991), and in situ hybridization (Butnaru et al, 1998). However, these methods are technically cumbersome and have their limitations.…”

Genetic and physical analysis of a single Festuca pratensis chromosome segment substitution in Lolium perenne

“…The RGs F, E and I (two genotypes) were progeny of G43; the remainder were progeny of G2 somal positions of markers and genes previously assigned by genetic analysis as a means of dissecting out traits and identifying new and potentially useful germplasm. This has largely been achieved using a combination of deletion mapping of cytogenetic and RFLP markers and in situ hybridisation (Song and Gustafson 1995;Wanous and Gustafson 1995;Lin et al 1997;Butnaru et al 1998;Sarma et al 2000); the current work in the Lolium/Festuca complex shares many of the same aims and contributes similar insights. However, the facility with which different introgression lines can be isolated, analysed and recombined within this latter system, together with the increasing complexity of the available genetic maps, means that the Lolium/Festuca complex is proving to be a valuable resource for high-resolution physical mapping of plant chromosomes and the basic understanding of recombination and alien introgression.…”

Physical mapping of restriction fragment length polymorphism (RFLP) markers in homoeologous groups 1 and 3 chromosomes of wheat by in situ hybridization

“…With improved detection methodologies, ISH has also been used for mapping low-or single-copy sequences on plant chromosomes Gustafson and Dillé 1992;Leitch and Heslop-Harrison 1993;Gustafson 1995, 1997;Ren et al 1997;Butnaru et al 1998;Ohmido et al 1998;Bi et al 1999;Biagetti et al 1999). However, the frequency of hybridization site detection with unique-sequence probes was extremely low (5-10%) and the hybridization site was often detected on only one chromatid.…”