Brief Reports: TRPM7 Senses Mechanical Stimulation Inducing Osteogenesis in Human Bone Marrow Mesenchymal Stem Cells

Xiao, E.; Yang, Hua-Qian; Gan, Ye‐Hua; Duan, Deng-Hui; He, Linxin; Guo, Yun-Bo; Wang, Shi Qiang; Zhang, Yi

doi:10.1002/stem.1858

Cited by 70 publications

(62 citation statements)

References 31 publications

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“…Mechanical stress is known to stimulate MSC differentiation [163][164][165]. In general, high tensile stress is associated with tenogenesis and low tensile stress with osteogenesis, while compressive loading is associated with chondrogenesis [166].…”

Section: Mechanical Stimulationmentioning

confidence: 99%

Strategies to engineer tendon/ligament-to-bone interface: Biomaterials, cells and growth factors

Tellado

Balmayor

Griensven

2015

Advanced Drug Delivery Reviews

221

216

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Section: Mechanical Stimulationmentioning

confidence: 99%

Strategies to engineer tendon/ligament-to-bone interface: Biomaterials, cells and growth factors

Tellado

Balmayor

Griensven

2015

Advanced Drug Delivery Reviews

221

216

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“…Both Drosophila Trpm and its mouse ortholog TRPM7 are reported to be constitutively active and permeable to a wide range of divalent cations (21,29). Mouse TRPM7 is known to respond to mechanical pressure (30,31), but further study will be needed to determine whether Drosophila Trpm is similarly responsive to mechanical triggers, such as those that occur during ovulation.…”

Section: The Drosophila Trpm Channel Plays Important Reproductive Rolesmentioning

confidence: 99%

DrosophilaTrpm mediates calcium influx during egg activation

Wolfner

2019

Preprint

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Egg activation is the process in which mature oocytes are released from developmental arrest and gain competency for embryonic development. In Drosophila and other arthropods, eggs are activated by mechanical pressure in the female reproductive tract, whereas in most other species, eggs are activated by fertilization. Despite the difference in the trigger, Drosophila shares many conserved features with higher vertebrates in egg activation, including a rise of intracellular calcium in response to the trigger. In Drosophila, this calcium rise is initiated by entry of extracellular calcium due to opening of mechanosensitive ion channels and initiates a wave that passes across the egg prior to initiation of downstream activation events. Here, we combined inhibitor tests, germline specific RNAi knockdown, and germline specific CRISPR/Cas9 knockout to identify the Transient receptor potential (TRP) channel subfamily M (Trpm) as a critical channel that mediates the calcium influx and initiates the calcium wave during Drosophila egg activation. We observed reduced egg hatchability in trpm germline knockout mutant females, although eggs were able to complete some egg activation events including cell cycle resumption. Since the mouse Trpm ortholog was recently reported also to be involved in calcium influx during egg activation and in further embryonic development our results suggest that calcium uptake from the environment via TRPM channels is a deeply conserved aspect of egg activation. SignificanceA rise in intracellular free calcium is a conserved feature of the egg-to-embryo transition in almost all animals. In Drosophila, as in vertebrates, the rise starts at one end of the egg, and then travels across the egg in a wave. The Drosophila calcium rise is mediated by an influx of calcium, due to the action of mechanically-gated ion channels. Here we identify the ion channel that is critical for the calcium entry as TRPM. TRPM is the ortholog of the channel recently

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“…Human bone mesenchymal stem cells (BMSC) and periodontal ligament (PDL) stem cells were cultured as previously described [53,54]; human dental pulp stem cells (DPSC) and adipose-derived stem cells (ASC) were incubated in Dulbecco’s modified Eagle’s medium (GIBCO) with 10% fetal bovine serum at 37 °C under 5% CO 2 . Human umbilical vein endothelial cells (HUVECs) were cultured at 37 °C with 5% CO 2 in medium 200 with low serum growth supplement (LSGS) kit (GIBCO).…”

Section: Methodsmentioning

confidence: 99%

Inhibiting HDAC1 Enhances the Anti-Cancer Effects of Statins through Downregulation of GGTase-Iβ Expression

Gan

2017

IJMS

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Hydroxy-methyl-glutaryl-coenzyme A (HMG-CoA) reductase inhibitors, namely statins, are potential anti-tumor agents. Previously, we showed that a pan-histone deacetylase (HDAC) inhibitor enhances the anti-tumor effects of the HMG-CoA inhibitor. However, the underlying mechanisms were not fully understood. Cancer cell lines (CAL-27 and SACC-83) were exposed to pan-HDAC inhibitor, or HDAC1 inhibitor, or geranylgeranyl transferase type I (GGTase-I) inhibitor alone or in combination with statin. Cell viability, apoptosis, migration, and invasion were assessed by Cell Count Kit-8, 4′,6-diamidino-2-phenylindole staining, and transwell assay, respectively. A xenograft model was used for assessing tumor growth in vivo. Western blot and real-time PCR were used to assess the expression of genes. We observed that inhibiting HDAC1 could enhance the anti-tumor effects of statins both in vitro and in vivo. Inhibiting HDAC1 blocked the statin-induced upregulation of geranylgeranyl transferase type Iβ subunit (GGTase-Iβ), resulting in an enhancement of the anti-cancer effects of statin. Overexpression of GGTase-Iβ or constitutively active RhoA abolished the enhancement by inhibiting HDAC1 on anti-tumor effects of statins. The HDAC1 inhibitor failed to enhance cytotoxicity in non-tumor primary cells treated with statin. Inhibiting HDAC1 enhanced the anti-cancer effects of statins through downregulation of GGTase-Iβ expression, and thus further inactivation of RhoA. A combination of statin with HDAC1 or GGTase-I inhibitor would be a new strategy for cancer chemotherapy.

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Brief Reports: TRPM7 Senses Mechanical Stimulation Inducing Osteogenesis in Human Bone Marrow Mesenchymal Stem Cells

Cited by 70 publications

References 31 publications

Strategies to engineer tendon/ligament-to-bone interface: Biomaterials, cells and growth factors

Strategies to engineer tendon/ligament-to-bone interface: Biomaterials, cells and growth factors

DrosophilaTrpm mediates calcium influx during egg activation

Inhibiting HDAC1 Enhances the Anti-Cancer Effects of Statins through Downregulation of GGTase-Iβ Expression

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