Broad bean wilt virus 1 encoded VP47 and SCP are suppressors of plant post-transcriptional gene silencing

Carpino, C.; Ferriol, Inmaculada; Elvira-González, Laura; Rubio, Luís; Peri, Ezio; Davino, Salvatore; Galipienso, Luis

doi:10.1007/s10658-020-02117-3

Cited by 5 publications

(8 citation statements)

References 34 publications

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“…All plants showed some necrotic spots in the in ltrated leaves due to the mechanical injuries produced during the in ltration process. These results are identical to those previously reported that described severe symptoms in N. benthamiana plants agroin ltrated with pBBWV1-Wt or mechanically infected with BBWV-1 and slight symptoms in equivalent plants agroin ltrated with pBBWV1-G492C (Carpino et al, 2020a;Medina et al, 2022).…”

Section: Resultssupporting

confidence: 92%

“…BBWV-1 cDNA infectious clones, Agrobacterium tumefaciens transformation and plant agroin ltration BBWV-1 cDNA infectious clones pBBWV1-Wt and pBBWV1-G492C (Carpino et al, 2020a) were used in this work. pBBWV1-Wt contained the full-length RNA1 and RNA2 of BBWV-1 isolate Ben into the binary plasmid pJL89 between the cauli ower mosaic virus (CaMV) 35S promoter and the nopaline synthase terminator (t-Nos).…”

Section: Methodsmentioning

confidence: 99%

“…Recently, the effect of BBWV-1 infection in N. benthamiana was studied by Transmission Electron Microscopy (TEM), reporting many subcellular changes in chloroplasts, plasmatic membrane, and mitochondria, and the presence of virus-like particles aggregates in inclusion bodies into the vacuoles (Medina et al, 2022). Furthermore, some of these ultrastructural alterations could be associated with the expression of BBWV-1 VP37 protein, which is a host speci city and pathogenicity determinant that was previously identi ed by using 35S-driven viral full-length cDNA infectious clone (pBBWV1-G4922C) knocking out the viral VP37 protein (Carpino et al, 2020a). In these experiments, N. benthamiana and broad bean plants agroin ltrated with pBBWV1-G492C showed milder leaf mosaic symptoms compared with those agroin ltrated with the wilt type cDNA infectious clone (pBBWV1-Wt).…”

Section: Introductionmentioning

confidence: 98%

“…RNA 2 (3.4 kb) has two "in frame" successive translation initiation codons encoding for the alternative polyproteins that differ only in their N-terminal extension length (Chen & Bruening, 1992;Lekkerkerker et al, 1996;Qi et al, 2002;Xie et al, 2016). Both polyproteins are processed by proteolytic cleavage rendering the large and the small coat protein (LCP and SCP, respectively) and two putative movement proteins (MPs) of different sizes but with overlapping C-terminal regions, with 47 and 37 kDa (VP47 and short VP37, respectively) (Carpino et al, 2020a).…”

Section: Introductionmentioning

confidence: 99%

“…In these experiments, N. benthamiana and broad bean plants agroin ltrated with pBBWV1-G492C showed milder leaf mosaic symptoms compared with those agroin ltrated with the wilt type cDNA infectious clone (pBBWV1-Wt). In addition, pBBWV1-G492C were unable to infect systemically tomato and pepper whereas pBBWV1-Wt did it (Carpino et al, 2020a). Transient expression in N. benthamiana 16C plants revealed that VP37 and LCP are viral suppressors (VSR) of the post-transcriptional gene silencing pathway (PTGS) (Carpino et al, 2020b).…”

Section: Introductionmentioning

confidence: 99%

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Proteomic insights into the effect of Broad bean wilt virus-1 infection in Nicotiana benthamiana plants

Elvira-González

Carpino

Rubio

et al. 2023

Preprint

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Broad bean wilt virus 1 (BBWV-1, genus Fabavirus, family Secoviridae) infects many plants species, including important horticultural crops. Since some tobacco plants such as Nicotiana benthamiana, are susceptible to many viruses, they are used as experimental hosts and therefore there is available abundant information about these species in databases. Here, the protein differential expression in N. benthamiana plants infected with BBWV-1 was studied using Liquid Chromatography coupled with Mass Spectrometry in tandem analysis (LC-MS/MS). Also, we studied the role of viral VP37 protein which is a BBWV-1 determinant of pathogenicity in the accumulation of the host proteins. For this purpose, we agroinfiltrated N. benthamiana plants with two BBWV-1 cDNA infectious clones: pBBWV1-Wt wilt type and the pBBWV1-G4922C mutant knocking out for the viral VP37 protein. Virus infection induced the differential expression of 44 host proteins: 22 were overexpressed and the other 22 were underexpressed. These proteins were involved in important plant processes and located in different cell organelles, mainly in chloroplasts. Finally, it was identified 24 proteins related to viral VP37 protein comparing the differential expression between N. benthamiana plants agroinfiltrated with pBBWV1-wt and pBBWV1-G4922C. Relation among host proteins that were differentially expressed, plant symptoms, and subcellular alterations are discussed.

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Section: Resultssupporting

confidence: 92%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Proteomic insights into the effect of Broad bean wilt virus-1 infection in Nicotiana benthamiana plants

Elvira-González

Carpino

Rubio

et al. 2023

Preprint

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Subcellular effects of broad bean wilt virus 1 infection in Nicotiana benthamiana and broad bean plants

Medina

Carpino²,

Elvira-González³

et al. 2022

Eur J Plant Pathol

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Broad bean wilt virus 1 (BBWV-1) from the genus Fabavirus (family Secoviridae) infects many important crops worldwide. We have studied the subcellular alterations in leaf tissues of Nicotiana benthamiana and broad bean (Vicia faba) plants infected by BBWV-1 using transmission electron microscopy (TEM). In addition, we have studied the effect at the subcellular level of the BBWV-1 VP37 protein, which is a pathogenicity and host specificity determinant. For this, we agroinfiltrated N, benthamiana, and broad bean plants with two BBWV-1 infective clones, the wild type pBBWV1-wt and pBBWV1-G492C defective for VP37. Mesophyll cells infected with BBWV1-wt showed many subcellular alterations such as starch accumulation and plastoglobuli in chloroplasts, cytoplasmic vesiculation, plasmalemmasomes, vacuolar inclusion bodies, and presence of virus-like particles (VLPs) scattered in the cytoplasm in both N. benthamiana and broad bean. As differential effects, aggregated VLPs in vacuolar inclusion bodies and mitochondrial proliferation were observed in N. benthamiana but not in broad bean. TEM observation of pBBWV1-G492C infected tissues suggested that VP37 could be involved in the starch accumulation in Revised version of the manuscriptClick here to access/download;Manuscript;Medina et al., BBWV-1 TEM (VRew Def).docx Click here to view linked References both N. benthamiana and broad bean, and with the presence of aggregated VLPs in vacuolar inclusion bodies, plasmalemmasomes, and mitochondrial proliferation in N. benthamiana. Thus, subcellular alterations induced by BBWV-1 depended on the host species and the effect of VP37 expression was higher in N. benthamiana than in broad bean.

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Proteomic insights into the effect of Broad bean wilt virus-1 infection in Nicotiana benthamiana plants

Elvira-González,

Carpino,

Rubio

et al. 2023

Eur J Plant Pathol

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Broad bean wilt virus 1 encoded VP47 and SCP are suppressors of plant post-transcriptional gene silencing

Cited by 5 publications

References 34 publications

Proteomic insights into the effect of Broad bean wilt virus-1 infection in Nicotiana benthamiana plants

Proteomic insights into the effect of Broad bean wilt virus-1 infection in Nicotiana benthamiana plants

Subcellular effects of broad bean wilt virus 1 infection in Nicotiana benthamiana and broad bean plants

Proteomic insights into the effect of Broad bean wilt virus-1 infection in Nicotiana benthamiana plants

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