Broad-specific immunochromatography for simultaneous detection of various sulfonylureas in adulterated multi-herbal tea

Xie, Haihuan; Li, Yingying; Jin, Wang; Lei, Yi; Koidis, Anastasios; Li, Xiangmei; Shen, Xing; Xu, Zhen-Lin; Lei, Hongtao

doi:10.1016/j.foodchem.2021.131055

Cited by 20 publications

(16 citation statements)

References 34 publications

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“…Structural similarity is often considered as the principle of immune hapten selection to induce broad-specific antibodies [ 28 ]. According to the previous reports on immunoassays for neonicotinoids, antibodies derived from imidacloprid hapten could be the most broad-selective, with diverse cross-reactivity to acetamiprid, thiacloprid, nitenpyram, imidaclothiz or clothianidin [ 14 , 17 , 18 ].…”

Section: Resultsmentioning

confidence: 99%

Trace Immunosensing of Multiple Neonicotinoid Insecticides by a Novel Broad-Specific Antibody Obtained from a Rational Screening Strategy

et al. 2022

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Residues of neonicotinoid pesticides have potential risks to food, environmental and biological safety. In this study, the hapten toward imidacloprid was adopted to gain antibodies. After molecular modeling, the electrostatic potentials of eight commonly-used neonicotinoid pesticides were individually calculated to analyze the structural similarity. Two representative compounds (imidacloprid and acetamiprid) with moderate similarity were rationally selected for hybridoma screening. Using this strategy, four clones of broad-specific monoclonal antibodies (mAbs) against multiple neonicotinoids were obtained, and the clone 6F11 exhibited the broadest spectrum to six neonicotinoid pesticides and two metabolites, with half-maximal inhibitory concentrations (IC50) ranging from 0.20 to 5.92 ng/mL. Then, the novel antibody gene was sequenced and successfully expressed in full-length IgG form using mammalian cells. Based on the sensitive recombinant antibody, a gold lateral-flow immunosensing strip assay was developed and it was qualified for rapid detection of imidacloprid, clothianidin or imidaclothiz residues in food samples.

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Section: Resultsmentioning

confidence: 99%

Trace Immunosensing of Multiple Neonicotinoid Insecticides by a Novel Broad-Specific Antibody Obtained from a Rational Screening Strategy

et al. 2022

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“…The similarity of the electrostatic isosurface between the hapten and the drugs can improve the possibility of obtaining broad-specificity Abs. 14 The alcohol hydroxyl group on the benzene ring in S-(+)-SAL had a positive charge. The ester group formed after the introduction of the spacer arm was lower than that of the alcoholic hydroxyl in the S-(+)-SAL.…”

Section: Resultsmentioning

confidence: 99%

“…13 The other is the preparation of a broad-specificity Ab that cross-reacts with a class of structural analogue. 14 The latter is more adopted for the broad-spectrum immunoassay of β-agonists (Table S1). For example, Degand et al 15 obtained a monoclonal antibody (mAb) by using succinic anhydride (SA) as a spacer arm, connecting it to the alcohol hydroxyl group at position 3 of the benzene ring of Racemic-SAL (Rac-SAL), which can recognize 5 β-agonists.…”

Section: Introductionmentioning

confidence: 99%

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Ultrasensitive Magnetic Assisted Lateral Flow Immunoassay Based on Chiral Monoclonal Antibody against R-(−)-Salbutamol of Broad-Specificity for 38 β-Agonists Detection in Swine Urine and Pork

Liang

Liu

Xie³

et al. 2022

J. Agric. Food Chem.

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Screening for ″zero tolerance″ β-agonists requires broad-specificity and sensitivity methods. Herein, R-(−)-salbutamol (SAL) is chirally separated and designed as a hapten, and a monoclonal antibody (mAb) was first prepared with an IC50 of 0.27 ng/mL, which can recognize 38 β-agonists simultaneously. The broad-specificity of chiral mAb was explored by molecular simulation technology. Magnetic nanoparticles (MNPs) were then synthesized and applied as a signal tracer to develop a lateral flow immunoassay (LFIA). The limits of detection of MNPs-LFIA for SAL in swine urine and pork were 0.05 and 0.09 μg/kg, which was (2–125)-fold lower than that of the reported LFIAs. The recoveries were between 95.8 and 116.7%, with the coefficient of variation from 2.7 to 15.4%. Parallel analysis of 44 samples by commercial ELISA kits confirmed the reliability. Therefore, our work not only provides a broad-specificity and ultrasensitive method for β-agonists but also suggests that chirality is the new general theory that guided the rational hapten design.

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“…The standard tolbutamide, glyburide, glipizide, glimepiride, gliquidone, and gliclazide were dissolved in MeOH with a concentration from 1.0~1000.0 ng/mL, respectively. As mentioned in a previous article [ 17 ], LC-MS/MS analysis was performed on AB QTRAP4500 triple quadrupole mass spectrometer.

…”

Section: Methodsmentioning

confidence: 99%

Complementary Strategy Enhancing Broad-Specificity for Multiplexed Immunoassay of Adulterant Sulfonylureas in Functional Food

Xie²,

et al. 2022

Biosensors

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Sulfonylureas, a family of anti-diabetic drugs widely used in the clinical treatment of type 2 diabetes, have recently emerged as an illegal adulterant in functional foods, to enhance the claimed anti-diabetic activity. To establish a screening assay method against their adulteration, with the aid of molecular simulation of hapten, two antibodies were raised and complementarily used to enhance the broad-specificity of an enzyme-linked immunosorbent assay (ELISA), which demonstrated simultaneous detection capability to 6 sulfonylureas; the detection limits ranged from 0.02 to 1.0 ng/mL, and recoveries were between 78.3% to 104.5%. Liquid chromatography with tandem mass spectrometry (LC-MS/MS) confirmed the reliability of the proposed ELISA, based on real samples. These results suggest that the proposed ELISA could be an ideal method for screening to monitor for illicit adulteration of sulfonylureas in functional pill products.

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Broad-specific immunochromatography for simultaneous detection of various sulfonylureas in adulterated multi-herbal tea

Cited by 20 publications

References 34 publications

Trace Immunosensing of Multiple Neonicotinoid Insecticides by a Novel Broad-Specific Antibody Obtained from a Rational Screening Strategy

Trace Immunosensing of Multiple Neonicotinoid Insecticides by a Novel Broad-Specific Antibody Obtained from a Rational Screening Strategy

Ultrasensitive Magnetic Assisted Lateral Flow Immunoassay Based on Chiral Monoclonal Antibody against R-(−)-Salbutamol of Broad-Specificity for 38 β-Agonists Detection in Swine Urine and Pork

Complementary Strategy Enhancing Broad-Specificity for Multiplexed Immunoassay of Adulterant Sulfonylureas in Functional Food

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