Bromoenol lactone enhances the permeabilization of rat submandibular acinar cells by P2X<sub>7</sub> agonists

Chaib, Naima; Kabré, Elie; Alzola, Eduardo; Pochet, Stéphanie; Dehaye, Jean-Paul

doi:10.1038/sj.bjp.0703124

Cited by 25 publications

(17 citation statements)

References 33 publications

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“…Previous work by us (6,10) and others (9,14,26,28) has shown that BzATP is as effective as 1 mM ATP in increasing [Ca 2ϩ ] i in salivary gland acinar and duct cells. In the present work, we found that 0.1 mM BzATP activated only a small inward current in acinar and duct cells, comparable with the current activated by 1 mM ATP, but much smaller than that activated by 5 mM ATP.…”

Section: P2rs In Parotid Glandmentioning

confidence: 99%

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Cell-specific Behavior of P2X7 Receptors in Mouse Parotid Acinar and Duct Cells

Luo

Zeng

et al. 2003

Journal of Biological Chemistry

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Rather, disassembly or solidification of the actin cytoskeleton prior to incubation with ATP prevented channel assembly. Remarkably, after completion of the slow activation, manipulation of the actin cytoskeleton no longer affected gating by ATP. Accordingly, manipulation of the actin cytoskeleton had no effect on P2X7R gating by ATP in duct cells. We concluded that P2X7Rs are not active in resting acinar cells. On exposure to ATP, P2X7Rs are assembled into functional channels with the aid of the actin cytoskeleton. Once assembled, P2X7Rs are subject to rapid gating by ATP. Duct cell P2X7Rs are preassembled and therefore continually subject to rapid gating by ATP. This cell-specific behavior may reflect the specific function of P2X7Rs in the two cell types.

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Section: P2rs In Parotid Glandmentioning

confidence: 99%

“…4, B and C). Brilliant Blue G (BBG) is a relatively specific P2X7R inhibitor (1,35) that was shown to inhibit P2X7Rs in parotid acinar cells (24,28). Fig.…”

Section: P2rs In Parotid Glandmentioning

confidence: 99%

Cell-specific Behavior of P2X7 Receptors in Mouse Parotid Acinar and Duct Cells

Luo

Zeng

et al. 2003

Journal of Biological Chemistry

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“…Although the P2X 7 itself is considered as a nonselective large pore, some groups have had difficulty in detecting it in cells expressing the heterologous P2X 7 receptor, suggesting that the pore-forming molecule might be a distinct entity (11,14,77). Similarly, some cell types present a native P2 receptor that shares most of the P2X 7 receptor pharmacological features but fail to permeabilize (43,62).…”

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confidence: 99%

Pharmacological properties of a pore induced by raising intracellular Ca²⁺

Faria¹,

Reis²,

Casabulho³

et al. 2009

American Journal of Physiology-Cell Physiology

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-Recent studies on the P2X 7 receptor in 2BH4 cells and peritoneal macrophages have demonstrated that the raise in intracellular Ca 2ϩ concentration induces a pore opening similar to P2X 7 receptor pore. Herein, we have investigated whether the pore activated by the elevation of intracellular Ca 2ϩ concentration is associated to P2X 7 receptor. Using patch clamp in cell attached, whole cell configuration, and dye uptake, we measured the pore opening in cell types that express the P2X 7 receptor (2BH4 cells and peritoneal macrophages) and in cells that do not express this receptor . In 2BH4 cells, the stimulation with ionomycin (5-10 M) increased intracellular free Ca 2ϩ concentration and induced pore formation with conductance of 421 Ϯ 14 pS, half-time (t 1 ⁄2) for ethidium bromide uptake of 118 Ϯ 17 s, and t 1 ⁄2 for Lucifer yellow of 122 Ϯ 11 s. P2X 7 receptor antagonists did not block these effects. Stimulation of HEK-293 and IT45-RI cells resulted in pore formation with properties similar to those found for 2BH4 cells. Connexin hemichannel inhibitors (carbenoxolone and heptanol) also did not inhibit the pore-induced effect following the increase in intracellular Ca 2ϩ concentration. However, 5-(N,N-hexamethylene)-amiloride, a P2X 7 receptor pore blocker, inhibited the induced pore. Moreover, intracellular signaling modulators, such as calmodulin, phospholipase C, mitogen-activated protein kinase, and cytoskeleton components were important for the pore formation. Additionally, we confirmed the results obtained for electrophysiology by using the flow cytometry, and we discarded the possibility of cellular death induced by raising intracellular Ca 2ϩ at the doses used by using lactate dehydrogenase release assay. In conclusion, increased concentration in intracellular Ca ϩ2 induces a novel membrane pore pharmacologically different from the P2X 7 associated pore and hemigap-junction pore.P2X 7 receptor; pore formation; second messenger P2X RECEPTORS are members of a family of P2 receptors with seven subtypes (P2X 1 -P2X 7 ) that induce an increase in intracellular Ca 2ϩ concentration when activated. Among the P2X receptors, P2X 7 subtype is the most divergent component of this family in terms of pharmacological properties, molecular structure, and function (54). This receptor forms a nonselective cation channel upon low ATP concentration, whereas at high ATP concentration, this receptor induces a pore formation that allows the flux of molecules of up to 900 Da and may lead to cell death. Although P2X 7 receptor is associated with expression of both functional channels and large pores, the structure of the large pore-forming subunits and whether the channel and the large pore are the same structure are still not known.From a physiological point of view, P2X 7 is mainly expressed in the immune system found in all cells of this system studied so far. The activation of P2X 7 receptor has several effects in the immune system such as release of mature interleukin-␤ and other pro-inflammatory cytokines (interleukin-18,...

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“…These results suggested that P2X7R could activate both calciumdependent and calcium-independent PLA2 in submandibular gland cells and epithelial cells. The iPLA2 was also associated with P2X7R permeabilization in submandibular gland acinar cells, and this permeabilization was inhibited by BEL [60]. In submandibular gland ductal cells, P2X7R induced kallikrein secretion in an iPLA2-dependent manner [28].…”

Section: P2x7r and The Lipid Metabolism Signaling Pathwaymentioning

confidence: 95%

“…In submandibular gland ductal cells, P2X7R induced kallikrein secretion in an iPLA2-dependent manner [28]. However, the data related to BEL in P2X7R-iPLA inhibition must be evaluated with caution because Chaid et al have described how BEL can enhance the permeabilization to ethidium bromide in submandibular acinar cells by acting directly on P2X7R [60].…”

Section: P2x7r and The Lipid Metabolism Signaling Pathwaymentioning

confidence: 99%

Lipid metabolism modulation by the P2X7 receptor in the immune system and during the course of infection: new insights into the old view

Costa-Junior

Marques-da-Silva

Vieira

et al. 2011

Purinergic Signalling

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For decades, scientists have described numerous protein pathways and functions. Much of a protein's function depends on its interactions with different partners, and those partners can change depending on the cell type or system. The P2X7 receptor (P2X7R) is one such multifunctional protein that is related to multiple partners and signaling pathways. The relationship between P2X7R and different enzymes involved in lipid metabolism represents a relatively new field in P2X7R research. This field of research began in epithelial cells and currently includes immune and nervous cells. The P2X7R-lipid metabolism pathway is related to many biological functions of P2X7R, such as cell death and pathogen clearance, and this signaling pathway may be involved in many functions that are dependent on bioactive lipids. In the present review, we will attempt to summarize data related to the P2X7R-lipid metabolism pathway, focusing on signaling pathways and their biological relevance to the immune system and infection.

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Bromoenol lactone enhances the permeabilization of rat submandibular acinar cells by P2X₇ agonists

Cited by 25 publications

References 33 publications

Cell-specific Behavior of P2X7 Receptors in Mouse Parotid Acinar and Duct Cells

Cell-specific Behavior of P2X7 Receptors in Mouse Parotid Acinar and Duct Cells

Pharmacological properties of a pore induced by raising intracellular Ca²⁺

Lipid metabolism modulation by the P2X7 receptor in the immune system and during the course of infection: new insights into the old view

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Bromoenol lactone enhances the permeabilization of rat submandibular acinar cells by P2X7 agonists

Cited by 25 publications

References 33 publications

Cell-specific Behavior of P2X7 Receptors in Mouse Parotid Acinar and Duct Cells

Cell-specific Behavior of P2X7 Receptors in Mouse Parotid Acinar and Duct Cells

Pharmacological properties of a pore induced by raising intracellular Ca2+

Lipid metabolism modulation by the P2X7 receptor in the immune system and during the course of infection: new insights into the old view

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Bromoenol lactone enhances the permeabilization of rat submandibular acinar cells by P2X₇ agonists

Pharmacological properties of a pore induced by raising intracellular Ca²⁺