BRONCHIAL PROVOCATION TESTS IN ATOPIC PATIENTS WITH ALLERGEN-SPECIFIC IgG4 ANTIBODIES

The adjuvant effect of silica and IL-1 in the development of late asthmatic responses (LARs) in guinea pigs was studied. Different doses of silica or recombinant human (rh) IL-1 (1 μg) with Ascaris suum were used for the immunization. The serum IL-1 concentration was measured after the immunization. One week after the immunization, antigen was challenged and respiratory resistance (Rrs) was measured. Rrs increased silica dose dependently in the late phase, and the increment of Rrs in the late phase was significantly correlated with the serum IL-1 concentration (p < 0.05). In addition, rhIL-1 administration showed an increase in Rrs at the late phase. Antigen-specific IgE and IgG1 were also measured and were increased in guinea pigs immunized both with silica and rhIL-1.

Section: Resultssupporting

confidence: 64%

Adjuvant Effect of lnterleukin-1 on the Development of Late Asthmatic Response in Guinea Pigs

Okada¹,

Inoue²,

Asano³

et al. 1993

“…Are IgG4 blocking antibodies? The importance of this subclass in allergic disease has been stressed by many authors [18,[26][27][28][29][30][31][32][33][34][35]. In our work, the sera of the patients were analyzed before any clinical treatment, and we cannot make a conclu sion on the role of these IgG4.…”

Section: Discussionmentioning

confidence: 87%

Characterization of Grass Pollen-Specific IgE, IgA, IgM Classes and IgG Subclasses in Allergic Patients

Desvaux

Peltre²,

David³

1989

Water-soluble grass pollen extracts (Dactylis glomerata) were separated by isoelectric focusing in a wide pH range (2–11) in agarose gel. After focusing, two successive gel prints were taken. The first one, on an ordinary nitrocellulose filter during 10 s, enabled the visualization of separated components of the pollen, after india ink staining. The second one, on a cyanogen bromide-activated nitrocellulose filter obtained after a 10-min transfer and saturation step, was incubated overnight with patient sera, and the specific antibodies bound to antigens or allergens were detected. The screening of different patient sera showed great variability in the antigen spectra. There was no obvious relationships between IgM and IgA antigen spectra as compared to IgE. Some association between IgE and IgG4 subclass was observed.

“…It was shown by Gwynn et al [4] that allergen-spe cific IgG4 antibodies are important in the production of late reaction. IgGI, IgG2 and IgG3 levels are not different between the group fo isolated immediate re sponse and the group of dual reaction [5].…”

Section: Discussionmentioning

confidence: 99%

“…However, the mechanism of late reactions is not clear as yet. The rel evance of IgG4 antibodies to late reaction has been reported [4], It has been shown also that chemical me diators probably from mast cells are increased by im mediate and late reactions [5,6].…”

Section: Introductionmentioning

confidence: 90%

IgG1 Antibodies to House Dust Mite <i>(Dermatophagoides farinae) </i>and Late Asthmatic Response

Izutsu¹,

Kudo²,

Okudaira³

et al. 1986

Thirteen asthmatic patients sensitive to mite were challenged by inhalation of an extract of mites (Dermatophagoides farinae). Seven showed dual bronchial reactions and 5 showed isolated immediate responses. No patient showed an isolated late reaction. Six of seven patients with dual reaction had higher IgG1 antibodies than the 5 patients with isolated immediate reaction when examined before the challenge. A similar result was obtained in terms of levels of immune complex. IgE, IgG4 and total IgG antibodies were not predictive for late reaction. These results suggest that there is a close correlation of the presence of high IgG1 antibodies with a propensity to develop late asthmatic responses. The meaning of this observation is discussed.