Brucella abortus cytoplasmic proteins used as antigens in an ELISA potentially useful for the diagnosis of canine brucellosis

Baldi, Pablo C.; Wanke, M.M.; Loza, Maria E.; Fossati, Carlos A.

doi:10.1016/0378-1135(94)90142-2

Cited by 29 publications

(27 citation statements)

References 11 publications

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“…The indirect ELISA has detected 10% sero-prevalence rate of brucellosis in stray dogs which supports the earlier reports of Radojivic et al (2001) who reported 9.37% and 10.87% sero-prevalence of brucellosis in stray dogs of Podgoria and Belgrade, respectively. However, higher (Diker et al, 1987;Molnar et al, 2001;Khairani-Bejo and Bahaman, 2006) and lower (Myers and Varela-Diaz, 1980;Baldi et al, 1994;Taner et al, 2005) seroprevalence rates of brucellosis have been reported in unattended dogs elsewhere.…”

Section: Resultsmentioning

confidence: 99%

Comparative Evaluation of Commercial Serodiagnostic Tests for the Seroprevalence Study of Brucellosis in Stray Dogs in Bangladesh

Talukder

Samad

Rahman

2012

Bangl. J. Vet. Med.

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A prospective study of Brucella sero-prevalence was conducted in 30 stray dogs in Mymensingh Municipal Corporation area of Bangladesh during the period from January to December 2009. All the sera were screened primarily by Rose Bengal Plate Test (RBPT). Positive, doubtful and negative serum samples were further confirmed with Slow Agglutination Test (SAT), Standard Tube Agglutination Test (STAT) and indirect Enzyme Linked Immunosorbent Assay (ELISA) and all the SAT, STAT and ELISA negative samples were confirmed as negative. The sensitivity of RBPT, SAT and STAT was found to be of 100%, 66.67% and 66.67% with considering ELISA as a standard test while specificity was found to be of 96.29%, 100% and 100% in stray dogs. This indicates that the SAT and STAT are found more specific than RBPT. Evaluation of comparison of sero-prevalence rates of canine brucellosis with the four sero-tests advocated to use ELISA for diagnosis of canine brucellosis. The overall sero-prevalence of canine brucellosis was recorded as 13.33%, 6.67%, 6.67% and 10.0% with RBPT, SAT, STAT and ELISA, respectively. Sero-results have analyzed on the basis of two age groups (up to 6 months and 7 to 36 months) and sex (male and female). Significantly (p<0.01) higher sero-prevalence rate of canine brucellosis was recorded in stray dogs aged between 7 to 36 months (14.81%, 7.40%, 7.40% and 11.11%) in comparison to aged group up to 6 months ( 0%, 0%, 0% and 0% ) with RBPT, SAT, STAT and ELISA, respectively. The sero-prevalence rate of canine brucellosis was found significantly (p <0.01) higher in female dogs (15.78%, 10.52%, 10.52% and 15.78%) in comparison to male (9.09%, 0%, 0% and 0%) with RBPT, SAT, STAT and ELISA, respectively. It appears from this study that brucellosis is an endemic disease and measures are needed to reduce this high prevalence of canine brucellosis in Bangladesh.

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Section: Resultsmentioning

confidence: 99%

Comparative Evaluation of Commercial Serodiagnostic Tests for the Seroprevalence Study of Brucellosis in Stray Dogs in Bangladesh

Talukder

Samad

Rahman

2012

Bangl. J. Vet. Med.

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“…In Brucella spp. research, cytoplasmic proteins of the bacteria have been shown to be used as antigens [58,59]. The deficiency of the gene may contribute to inhibition of bacterial antigens which are used to recognize infection in the host cells.…”

Section: C32 -Nod-like Signaling Pathway 11mentioning

confidence: 99%

Genes Related to Intracellular Survival of Brucella abortus in THP-1 Macrophage Cells

Shim

Jung

et al. 2018

Journal of Microbiology and Biotechnology

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Brucella abortus can survive and replicate within host macrophages, and great efforts have been made to demonstrate the genes involved in pathogenicity, such as internalization, in Brucella research. Here, intracellular responses were compared between THP-1 macrophage cells stimulated with B. abortus wild-type and four mutants (C1, C10, C27, and C32) using microarray to demonstrate the role of genes related to intracellular survival and replication. These mutants were generated by deleting genes encoding BAB_RS13225 (4-hydrobenzoate 3monooxygenase, PHBH), BAB_RS00455 (heme exporter protein cytochrome C, CcmC), BAB_RS03675 (exopolyphosphatase, PPX), and BAB_RS13225 (peptidase M24). The results showed that mutants C1 and C10 induced significant suppression of survival levels and cytokine expression relative to wild-type in the THP-1 macrophage cells. These findings suggest that the BAB_RS13225 and BAB_RS00455 genes play important roles in survival within human macrophages. Conversely, mutants C27 and C32 induced significantly higher survival level than wild-type in the cells inhibiting cellular signal transduction. It is assumed that the BAB_RS03675 and BAB_RS13225 genes play a role in cellular resistance to B. abortus. Therefore, the disrupted genes are involved in B. abortus intracellular growth, and especially in its survival, and they could be effective targets for understanding the intracellular bacterium, B. abortus.

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“…While the latter had high levels of antibodies against the 18-kDa protein, the vaccinated cattle developed only a low and transient antibody response that was no longer detected at 90 days post-vaccination (a time when anti-LPS antibodies were still detected at high levels). The 18-kDa protein and the CP antigen were also useful for the diagnosis of canine brucellosis (6,8). The antibody reactivity to cytoplasmic proteins of Brucella was investigated by ELISA in sera from 30 dogs having confirmed or suspected brucellosis.…”

Section: Diagnostic Usefulness Of Brucella Cytoplasmic Proteinsmentioning

confidence: 99%

Structural, functional and immunological studies on a polymeric bacterial protein

Baldi

Velikovsky

Braden

et al. 2000

Braz J Med Biol Res

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The characterization of proteins from Brucella spp, the causative agent of brucellosis, has been the subject of intensive research. We have described an 18-kDa cytoplasmic protein of Brucella abortus and shown the potential usefulness of this protein as an antigen for the serologic diagnosis of brucellosis. The amino acid sequence of the protein showed a low but significant homology with that of lumazine synthases. Lumazine is an intermediate product in bacterial riboflavin biosynthesis. The recombinant form of the 18-kDa protein (expressed in E. coli) folds like the native Brucella protein and has lumazinesynthase enzymatic activity. Three-dimensional analysis by X-ray crystallography of the homolog Bacillus subtilis lumazine synthase has revealed that the enzyme forms an icosahedral capsid. Recombinant lumazine synthase from B. abortus was crystallized, diffracted X rays to 2.7-Å resolution at room temperature, and the structure successfully solved by molecular replacement procedures. The macromolecular assembly of the enzyme differs from that of the enzyme from B. subtilis. The Brucella enzyme remains pentameric (90 kDa) in its crystallographic form. Nonetheless, the active sites of the two enzymes are virtually identical at the structural level, indicating that inhibitors of these enzymes could be viable pharmaceuticals across a broad species range. We describe the structural reasons for the differences in their quaternary arrangement and also discuss the potential use of this protein as a target for the development of acellular vaccines. Correspondence

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Brucella abortus cytoplasmic proteins used as antigens in an ELISA potentially useful for the diagnosis of canine brucellosis

Cited by 29 publications

References 11 publications

Comparative Evaluation of Commercial Serodiagnostic Tests for the Seroprevalence Study of Brucellosis in Stray Dogs in Bangladesh

Comparative Evaluation of Commercial Serodiagnostic Tests for the Seroprevalence Study of Brucellosis in Stray Dogs in Bangladesh

Genes Related to Intracellular Survival of Brucella abortus in THP-1 Macrophage Cells

Structural, functional and immunological studies on a polymeric bacterial protein

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