BSA-coated magnetic nanoparticles for improved therapeutic properties

Aires, Antonio; Ocampo, Sandra M.; Cabrera, David; Cueva, Leonor de la; Salas, Gorka; Terán, Francisco J.; Cortajarena, Aitziber L.

doi:10.1039/c5tb00833f

Cited by 50 publications

(51 citation statements)

References 49 publications

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“…Results show that coating with BSA decreases the degradation of the nanoparticles and reduces the toxicity in the animal model [14]. Another study showed that BSA coating of an iron oxide core can improve the in vivo and in vitro therapeutic outcome for efficient cancer treatment [15,16].…”

Section: Introductionmentioning

confidence: 99%

Structured Magnetic Core/Silica Internal Shell Layer and Protein Out Layer Shell (BSA@SiO2@SME): Preparation and Characterization

et al. 2019

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Purpose Magnetic nanoparticles are an interesting approach in the biomedical and biotechnological field. They can be used as a drug delivery system or in magnetic resonance imaging. However, these particles have the disadvantage of being colloidally instable, easily oxidized, and suffer from partial toxicity. To overcome these problems, magnetic nanoparticles were coated by different types of coats such as silica, polymer, etc. the purpose of this study is to develop a coated iron oxide nanoparticle system. Methods Seed magnetic emulsion particles (SME) were first prepared and characterized before inducing silica layer using sol-gel process. The obtained SiO 2 @SME particles are then encapsulated using bovine serum albumin (BSA) layer. This proteins layer was performed via nanoprecipitation of BSA molecules on the SME. Particles were characterized by electron microscopy, FTIR, TGA, and zeta potential measurement. Results Characterization studies confirm the successful coating of BSA on the surface of amino-functionalized silica shell and magnetic core. Conclusion The used process leads to the preparation of highly magnetic particles encapsulated with silica layer ad then coated with proteins shell. The presence of silica shell will enhance the chemical stability of the magnetic core, whereas, the presence of proteins shell will improve low cytotoxicity and good biocompatibility in the contact with biological samples.

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Section: Introductionmentioning

confidence: 99%

Structured Magnetic Core/Silica Internal Shell Layer and Protein Out Layer Shell (BSA@SiO2@SME): Preparation and Characterization

et al. 2019

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“…Strategies to covalently anchor BSA require the modification of the initial SPIONs with tedious reactions with organic solvents (toxic for biological applications), the exchange of solvents and they restrict and fix the conformation of the anchored protein. 16,21 Non-covalent strategies require hydrophilic iron oxide surfaces, the common method of synthesis cannot offer it; and it adds an extra step. 16,17 In our case, we synthesized SPIONs using microwave-assisted thermal decomposition and capped them with citrate, rendering hydrophilic SPIONs in a single step.…”

Section: Introductionmentioning

confidence: 99%

Albumin-coated SPIONs: an experimental and theoretical evaluation of protein conformation, binding affinity and competition with serum proteins

Perálvarez-Marín

Minelli

et al. 2016

Nanoscale

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The variety of nanoparticles (NPs) used in biological applications is increasing and the study of their interaction with biological media is becoming more important. Proteins are commonly the first biomolecules that NPs encounter when they interact with biological systems either in vitro or in vivo. Among NPs, super-paramagnetic iron oxide nanoparticles (SPIONs) show great promise for medicine. In this work, we study in detail the formation, composition, and structure of a monolayer of bovine serum albumin (BSA) on SPIONs. We determine, both by molecular simulations and experimentally, that ten molecules of BSA form a monolayer around the outside of the SPIONs and their binding strength to the SPIONs is about 3.5 × 10(-4) M, ten times higher than the adsorption of fetal bovine serum (FBS) on the same SPIONs. We elucidate a strong electrostatic interaction between BSA and the SPIONs, although the secondary structure of the protein is not affected. We present data that supports the strong binding of the BSA monolayer on SPIONs and the properties of the BSA layer as a protein-resistant coating. We believe that a complete understanding of the behavior and morphology of BSA-SPIONs and how the protein interacts with SPIONs is crucial for improving NP surface design and expanding the potential applications of SPIONs in nanomedicine.

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“…Surface Modifi cation of ZnS:Mn/MnS Core-Shell NRs : To prepare the contrast agent solutions according to previous reports, [ 57,58 ] 10.0 mg of ZnS:Mn/MnS core-shell NRs powder was weighed and transferred into a glass vial. Ethylenediamine (Xiya Reagent) (2 mL) was added into the vial followed by the addition of 0.05 mL of ethanedithiol (J&K).…”

Section: Methodsmentioning

confidence: 99%

“…This demonstrates that the migration of Mn ions into the ZnS NRs for doping occurred prior to the coating of the MnS layer during the synthesis. [ 57,58 ] BSA has been one of the most extensively studied proteins because of its sequence and structural homology to human serum albumin, and it provides NCs with a better hemocompatibility, longer blood circulation times, as well as hindering the undesired adsorption of other proteins. However, the TEM image also shows lots of particles in the product, which were MnS nanoparticles generate by the excess Mn and S sources.…”

Section: Full Paper Full Paper Full Papermentioning

confidence: 99%

“…Interestingly, when the MnCl 2 ·4H 2 O amount was increased from 0.05 to 0.075 mmol, NRs with a diameter of 8-10 nm were observed in the TEM image ( Figure 5 c), indicating that the ZnS NRs were successfully coated with a MnS shell. [ 59,60 ] After being transferred, the obtained colloidal aqueous solution was stable with very few precipitation for 3 days ( Figure S1, Supporting Information). Moreover, the fl uorescent spectrum shown in Figure 5 b (black curve) did not demonstrate the characteristic emission of ZnS:Mn around 580 nm for this sample, which was likely caused by the fl uorescence quenching due to the excess Mn 2+ ions doped into the ZnS core.…”

Section: Full Paper Full Paper Full Papermentioning

confidence: 99%

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Synthesis of Ultrathin MnS Shell on ZnS: Mn Nanorods by One‐Step Coating and Doping for MRI and Fluorescent Imaging

Zhao

Meng

Sheng

et al. 2016

Advanced Optical Materials

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advantages of fl uorescence and MRI imaging, have attracted a great deal of attention in particular. [15][16][17] Semiconductor nanocrystals (or quantum dots) have also been of quite some interest over recent decades because of their unique optical properties and extensive applications. Compared to other inorganic fl uorescent materials, such as carbon dots, rare-earth upconversion nanocrystals, and noble-metal clusters, semiconductor nanocrystals have unique fl uorescent advantages, such as high quantum yields (QYs), long lifetime, and minimal self-absorption. [ 18,19 ] However, these semiconductor nanocrystals are not suited to widespread applications in bioimaging and cell labeling in vivo due to their high toxicity related to the intrinsic Cd (such as CdS and CdSe).Mn 2+ -doped semiconductor nanocrystal (NC) emitters have been extensively researched owing to their low toxicity and yellow emission at 570-580 nm, originating from the 4 T 1 -6 A 1 transition of the Mn 2+ on the Zn 2+ sites. [20][21][22][23][24] These properties refl ect the potential use of Mn-doped NCs as fl uorescence imaging agents. Schneider et al., for example, successfully prepared thioglycerol-capped Mn-doped ZnS quantum dots as a two-photon fl uorescent nanoprobe for fl uorescence imaging applications. [ 25 ] Wu et al. synthesized probes based on Mn-doped ZnS quantum dots to distinguish between oxidoreductase isozyme NQO1 expressing and non-expressing cancer cell lines. [ 26 ] Mn-doped ZnS quantum dots synthesized by Wu's team, when used as a new imaging moiety for eccrine latent fi ngerprints, revealed the material's potential applications in forensic science. [ 27 ] Fluorescent quantum dots of Mndoped ZnS, synthesized by Manzoor et al., also showed a high selectivity in targeted cancer imaging. [ 28 ] Because of its fi ve unpaired electrons, Mn 2+ can also produce an effi cient positive contrast enhancement as an alternative MRI contrast agent. [ 29,30 ] Compared to the commonly used Gd 3+ , Mn 2+ not only has a lower toxicity, but is also present as a trace element in ionic form in the human body. [ 31,32 ] The intrinsic properties of Mn include a high spin number, long electronic relaxation time, and good exchange with water, thus increasing the signal intensity of MRI T 1 images. At the biochemical level, Mn is involved in the mitochondrial functionthe greater the mitochondrial density in the cell, the higher the Multifunctional nanoprobes for bioimaging have attracted considerable interest in terms of their potential applications in clinical medicine and diagnosis. This article reports the fabrication of a new bifunctional nanoprobe, consisting of an ultrathin MnS shell coating on ZnS:Mn nanorods (NRs), for bioimaging in combination with fl uorescence and magnetic resonance imaging (MRI) techniques. The prepared ZnS:Mn/MnS core-shell NRs show an orange emission at 587 nm in wavelength, suggesting they are well suited for fl uorescent bio-imaging due to their large Stokes shift.After surface modifi cation, the obtained core-shell NR...

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BSA-coated magnetic nanoparticles for improved therapeutic properties

Cited by 50 publications

References 49 publications

Structured Magnetic Core/Silica Internal Shell Layer and Protein Out Layer Shell (BSA@SiO2@SME): Preparation and Characterization

Structured Magnetic Core/Silica Internal Shell Layer and Protein Out Layer Shell (BSA@SiO2@SME): Preparation and Characterization

Albumin-coated SPIONs: an experimental and theoretical evaluation of protein conformation, binding affinity and competition with serum proteins

Synthesis of Ultrathin MnS Shell on ZnS: Mn Nanorods by One‐Step Coating and Doping for MRI and Fluorescent Imaging

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