BSA fragmentation specifically induced by added electrolytes: An electrospray ionization mass spectrometry investigation

Lusci, Gloria; Pivetta, Tiziana; Carucci, Cristina; Parsons, Drew F.; Salis, Andrea; Monduzzi, Maura

doi:10.1016/j.colsurfb.2022.112726

Cited by 6 publications

(3 citation statements)

References 81 publications

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“…The suppression of organic fragment ions at high salinities might be because the ionization of fragments is suppressed; alternatively, charged fragments may be neutralized by the salts, or the fragmentation process of the organics may be suppressed. NaCl has been shown to have a stabilizing effect against fragmentation in ESI mass spectrometry . Besides, we detected more fragments in cation than anion mode (Tables S1 and S2).…”

Section: Discussionmentioning

confidence: 76%

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Mass Spectrometric Fingerprints of Organic Compounds in NaCl-Rich Ice Grains from Europa and Enceladus

Napoleoni

Klenner

Khawaja

et al. 2023

ACS Earth Space Chem.

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Europa and Enceladus, respective moons of Jupiter and Saturn, are prime targets in the exploration of potentially habitable extraterrestrial ocean worlds. Organic material could be incorporated from the ocean into ice grains ejected from the surface or in potential plumes and detected via spacecraft flybys with impact ionization mass spectrometers, such as the SUrface Dust Analyzer (SUDA) onboard Europa Clipper or the Cosmic Dust Analyzer (CDA) onboard the past Cassini mission. Ice grains ejected from both Europa and Enceladus are expected to contain sodium salts, specifically sodium chloride (NaCl), in varying concentrations. Consequently, it is important to understand its effects on the mass spectrometric signatures of organic material in salt-rich ice grains. Previous studies have only focused on the detection of biosignatures, such as amino acids, in salt-rich ice grains. We here perform analogue experiments using the Laser Induced Liquid Beam Ion Desorption (LILBID) technique to study how a wide variety of abiotic and potentially biotic organic molecules could be identified by SUDA-type instruments. We investigate their mass spectral characteristics and detectability at various typical NaCl concentrations expected for salt-rich ice grains and in both cation and anion modes. Results show that organics in salt-rich ice grains can still be detected because of the formation of molecular ions and sodiated and chlorinated species. However, high salt concentrations induce compound- and concentration-dependent suppression effects, depending on the chemical properties and functional groups of the analytes. Our results emphasize the need of both ion modes to detect a wide range of organics embedded in complex matrices and to discriminate between abiotic and potentially biotic species. This work complements a spectral reference library for Europa Clipper and other ocean world missions.

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Section: Discussionmentioning

confidence: 76%

“…NaCl has been shown to have a stabilizing effect against fragmentation in ESI mass spectrometry. 103 Besides, we detected more fragments in cation than anion mode (Tables S1 and S2). This is consistent with the generally higher sensitivity to organic cations than organic anions that we observed, which then also applies to fragments.…”

Section: Discussionmentioning

confidence: 94%

Mass Spectrometric Fingerprints of Organic Compounds in NaCl-Rich Ice Grains from Europa and Enceladus

Napoleoni

Klenner

Khawaja

et al. 2023

ACS Earth Space Chem.

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“…Ion specificity was discovered by Hofmeister in 1888 while studying salt-induced egg white protein precipitation/solubilization [1]. Since then, specific ion (Hofmeister) phenomena have been found in a myriad of bio-systems [2] including enzymes [3][4][5][6], proteins [7][8][9], lipids [10], and nucleotides [11][12][13][14]. To date, specific ion effects on biological systems are not fully understood [2,[15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Cation and buffer specific effects on the DNA-lipid interaction

Mura

Humphreys

Gilbert

et al. 2023

Colloids and Surfaces B: Biointerfaces

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MBG/BSA Bone Grafts Immunomodulate Bone Regeneration by Releasing Bioactive Ions in Inflammatory Bone Defects

Yang,

Gu,

Jiang

et al. 2024

Adv Healthcare Materials

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Since the diseases that cause bone defects are mostly inflammatory diseases, the current bone grafts are unable to effectively regulate osteoimmune activity, leading to the impaired osteogenesis and unfavorable bone regeneration. In this study, inspired by bone composition, biomimetic mesoporous bioactive glass nanoparticle (MBG)/bovine serum albumin (BSA) bone grafts are designed for inflammatory bone defects. Systematically, MBG/BSA bone grafts are evaluated for characterization, bioactivity, anti‐inflammatory, antioxidant activity, and osteogenic activity. MBG/BSA bone grafts are proved to be biocompatible and can release bioactive ions including calcium and silicon in a sustained manner. Furthermore, MBG/BSA reprograms the macrophage phenotype toward anti‐inflammation that is beneficial for bone regeneration. The antioxidative activity is also validated under inflammation and the mechanism may be via the interleukin‐4 (IL‐4)/Signal transducer and activator of transcription 6 (STAT6) pathway. The osteogenic differentiation and mineralization are also facilitated due to the improved immunoregulation of MBG/BSA. Overall, this work suggests that the MBG/BSA bone grafts with improved immunomodulatory properties are an ideal material for inflammatory bone regeneration application.

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BSA fragmentation specifically induced by added electrolytes: An electrospray ionization mass spectrometry investigation

Cited by 6 publications

References 81 publications

Mass Spectrometric Fingerprints of Organic Compounds in NaCl-Rich Ice Grains from Europa and Enceladus

Mass Spectrometric Fingerprints of Organic Compounds in NaCl-Rich Ice Grains from Europa and Enceladus

Cation and buffer specific effects on the DNA-lipid interaction

MBG/BSA Bone Grafts Immunomodulate Bone Regeneration by Releasing Bioactive Ions in Inflammatory Bone Defects

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