Btg1 and Btg2 gene expression during early chick development

Kamaid, Andrés; Giráldez, Fernando

doi:10.1002/dvdy.21616

Cited by 23 publications

(16 citation statements)

References 76 publications

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“…BTG2 belongs to the anti-proliferative gene family and has been shown to be involved in cell growth, differentiation and survival (Mo et al 2011), muscle fibre size, intramuscular fat deposition and weight loss (Kamaid and Giráldez 2008;Sasaki et al 2006). FA analyses demonstrated that x-3 and x-6 FAs increased in the liver of lambs supplemented at 10 and 20 % Spirulina levels.…”

Section: Discussionmentioning

confidence: 97%

Molecular genetics-nutrition interactions in the expression of AANAT, ADRB3, BTG2 and FASN genes in the heart, kidney and liver of Australian lambs supplemented with Spirulina (Arthrospira platensis)

Malau‐Aduli

Kashani

2015

Genes Genom

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Quantitative reverse transcriptase polymerase chain reaction was used to assess the messenger RNA (mRNA) transcription levels and expressions of Aralkylamine N-acetyltransferase, Adrenergic beta-3 receptor, B cell translocation gene 2, and Fatty acid synthase genes. In total, 432 heart, kidney and liver samples from 48 purebred and crossbred Australian prime lambs supplemented with Spirulina (Arthrospira platensis) blue-green cyanobacterial microalgae over a 9-week feeding trial were utilized for fatty acid composition and mRNA extraction. The aim was to assess the effect of dietary Spirulina supplementation on the mRNA expression profiles of genes controlling fatty acid metabolism in the heart, kidney and liver. Fourty-eight weaned lambs belonging to four breeds under the same management conditions were maintained on ryegrass pasture and randomly allocated to three treatment groups that comprised the control and two levels of Spirulina supplementation: Low (100 mL/head/day of 1 g Spirulina powder dissolved in 10 mL of water (10 % wt:vol)) and High (200 mL/head/day of 2 g of Spirulina dissolved in 10 mL of water (20 % wt:vol)). Both the low and high levels of Spirulina supplementation regimes strongly up-regulated the transcription of all the selected genes and increased x-3 and x-6 polyunsaturated fatty acid compositions in the tested organs. Breed and sex did not influence gene expression patterns; however, significant variations in response to Spirulina supplementation underpin the genetics-nutrition interactions that could be of practical importance for manipulating meat quality in the Australian dual-purpose prime lamb industry for a healthy polyunsaturated fatty acid profile. Abbreviations FAFatty acids AANAT Aralkylamine N-acetyltransferase ADRB3 Adrenergic beta-3 receptor BTG2 B-cell translocation gene 2 FASN Fatty acid synthase

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Section: Discussionmentioning

confidence: 97%

Molecular genetics-nutrition interactions in the expression of AANAT, ADRB3, BTG2 and FASN genes in the heart, kidney and liver of Australian lambs supplemented with Spirulina (Arthrospira platensis)

Malau‐Aduli

Kashani

2015

Genes Genom

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“…SIRT1, which was shown to be upregulated after irradiation, promotes transient and constituent Wnt signaling through regulation of Dishevelled proteins [ 66 ]. KRAS, HDAC1 and BTG2 (upregulated in the gene expression analysis) and p21 (upregulated using immunoblotting) are known to stimulate Wnt signaling [ 67 – 69 ]. SIAH-1 (upregulated in the gene expression analysis) is also activating Wnt signaling through activation of ß-catenin by ubiquitination [ 70 ].…”

Section: Discussionmentioning

confidence: 99%

Ionizing radiation induces immediate protein acetylation changes in human cardiac microvascular endothelial cells

et al. 2015

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Reversible lysine acetylation is a highly regulated post-translational protein modification that is known to regulate several signaling pathways. However, little is known about the radiation-induced changes in the acetylome. In this study, we analyzed the acute post-translational acetylation changes in primary human cardiac microvascular endothelial cells 4 h after a gamma radiation dose of 2 Gy. The acetylated peptides were enriched using anti-acetyl conjugated agarose beads. A total of 54 proteins were found to be altered in their acetylation status, 23 of which were deacetylated and 31 acetylated. Pathway analyses showed three protein categories particularly affected by radiation-induced changes in the acetylation status: the proteins involved in the translation process, the proteins of stress response, and mitochondrial proteins. The activation of the canonical and non-canonical Wnt signaling pathways affecting actin cytoskeleton signaling and cell cycle progression was predicted. The protein expression levels of two nicotinamide adenine dinucleotide (NAD+)-dependent deacetylases, sirtuin 1 and sirtuin 3, were significantly but transiently upregulated 4 but not 24 h after irradiation. The status of the p53 protein, a target of sirtuin 1, was found to be rapidly stabilized by acetylation after radiation exposure. These findings indicate that post-translational modification of proteins by acetylation and deacetylation is essentially affecting the radiation response of the endothelium.

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“…Moreover, Btg1 induces avian myoblast differentiation (Marchal et al, 1995; Rodier et al, 1999) and the development of endothelial cells (Iwai et al, 2004) and is also likely involved in the differentiation of sperm cells (Raburn et al, 1995). Btg1 is expressed in the developing and adult brain (Su et al, 2004; Kamaid and Giráldez, 2008), but no information on its function in neural tissues is available. Thus, we generated Btg1 knock out mice and analyzed the functional contribution by Btg1 to the adult neurogenic niches of the hippocampus and SVZ (Zhao et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Btg1 is Required to Maintain the Pool of Stem and Progenitor Cells of the Dentate Gyrus and Subventricular Zone

Farioli-Vecchioli¹,

Micheli²,

Saraulli³

et al. 2012

Front. Neurosci.

123

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Btg1 belongs to a family of cell cycle inhibitory genes. We observed that Btg1 is highly expressed in adult neurogenic niches, i.e., the dentate gyrus and subventricular zone (SVZ). Thus, we generated Btg1 knockout mice to analyze the role of Btg1 in the process of generation of adult new neurons. Ablation of Btg1 causes a transient increase of the proliferating dentate gyrus stem and progenitor cells at post-natal day 7; however, at 2 months of age the number of these proliferating cells, as well as of mature neurons, greatly decreases compared to wild-type controls. Remarkably, adult dentate gyrus stem and progenitor cells of Btg1-null mice exit the cell cycle after completing the S phase, express p53 and p21 at high levels and undergo apoptosis within 5 days. In the SVZ of adult (two-month-old) Btg1-null mice we observed an equivalent decrease, associated to apoptosis, of stem cells, neuroblasts, and neurons; furthermore, neurospheres derived from SVZ stem cells showed an age-dependent decrease of the self-renewal and expansion capacity. We conclude that ablation of Btg1 reduces the pool of dividing adult stem and progenitor cells in the dentate gyrus and SVZ by decreasing their proliferative capacity and inducing apoptosis, probably reflecting impairment of the control of the cell cycle transition from G1 to S phase. As a result, the ability of Btg1-null mice to discriminate among overlapping contextual memories was affected. Btg1 appears, therefore, to be required for maintaining adult stem and progenitor cells quiescence and self-renewal.

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Btg1 and Btg2 gene expression during early chick development

Cited by 23 publications

References 76 publications

Molecular genetics-nutrition interactions in the expression of AANAT, ADRB3, BTG2 and FASN genes in the heart, kidney and liver of Australian lambs supplemented with Spirulina (Arthrospira platensis)

Molecular genetics-nutrition interactions in the expression of AANAT, ADRB3, BTG2 and FASN genes in the heart, kidney and liver of Australian lambs supplemented with Spirulina (Arthrospira platensis)

Ionizing radiation induces immediate protein acetylation changes in human cardiac microvascular endothelial cells

Btg1 is Required to Maintain the Pool of Stem and Progenitor Cells of the Dentate Gyrus and Subventricular Zone

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