Bubaline Cholecyst Derived Extracellular Matrix for Reconstruction of Full Thickness Skin Wounds in Rats

Shakya, Poonam; Sharma, Ashok; Kumar, Naveen; Remya, V.; Mathew, Dayamon David; Dubey, Prasoon; Shrivastava, Sonal; Shrivastava, Sameer; Maiti, Swapan Kumar; Hasan, Anwarul; Singh, Kiranjeet

doi:10.1155/2016/2638371

Cited by 11 publications

(7 citation statements)

References 39 publications

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“…(Nanda et al, 2017;Nagar et al, 2016). Histological observation criteria for collagen and angiogenesis followed the previous studies (Shakya et al, 2016;Nasiri et al, 2015).…”

Section: Animal Experimentsmentioning

confidence: 95%

Effectiveness Of Incision Wound Healing Of Pandanus Amaryllifolius Roxb In Wistar Rats

Putri

Girsang²,

Chiuman³

et al. 2022

IJHP

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The incision is a condition of tissue discontinuity that is commonly encountered with the risk of prolonged healing time, imperfect wound healing, infection, etc. Assessment of the wound healing activity of the incision is the purpose of this research, which includes an examination of wound contraction, the thickness of collagen fibers, and the number of new blood vessels formation. In total, 25 rats will be divided into 5 test groups, each consisting of 5 rats; the control group (cream base treated), standard group (Povidone Iodine), followed by the treated group with 10%, 20%, and 30% pandan leaf extract ointment. The preliminary screening of phytochemical analysisshowed the presence of alkaloids, saponins, flavonoids, tannins, triterpenoids, and steroids and glycosides, with the total content of phenols and flavonoids from the ethanolic extract of Pandan Wangi is 20.95 GAE mg/gram extract, and 23.57 TAE mg/gram extract. Wound contraction examination result showed a significant difference between the treatment groups on day 16 and a sign the pairs of groups after being given the ointment with each concentration (p <0.05), especially in the group with 30% fragrant leaves extract and the standard group. Examination of new blood vessels showed a significant difference (p <0.05), especially with 30% of the leaves extract ointment. There was also a significant difference in collagen density in the group given pandanus ethanol ointment, especially at the 30% pandanus extract content against the group that was only given the ointment base. This research strongly suggests that the fragrant pandan leaf extract ointment is effective in healing incisional wounds and promises the potential for further studies on the pharmacological aspects.

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“…(Nanda et al, 2017;Nagar et al, 2016). Histological observation criteria for collagen and angiogenesis followed the previous studies (Shakya et al, 2016;Nasiri et al, 2015).…”

Section: Animal Experimentsmentioning

confidence: 95%

Effectiveness Of Incision Wound Healing Of Pandanus Amaryllifolius Roxb In Wistar Rats

Putri

Girsang²,

Chiuman³

et al. 2022

IJHP

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“…The specimens were colored with hematoxylin eosin. The observation criteria of histological feature are collagen : none : score = 0, low amount : score = 1, moderate : score = 2, and high amount : score = 3; the number of polymorphonuclear polymorphonuclear (PMN): none : score = 0, low amount : score = 1, moderate : score = 2, and high amount : score = 3; degree of angiogenesis: none : score = 0, less than 5 veins : score = 1, 6–10 veins : score = 2, and more than 10 veins : score = 3; and re-epithelization, none : score = 0, partial : score = 1, complete but with immature epithelium : score = 2, and complete with mature epithelium : score = 3 [17,18].…”

Section: Methodsmentioning

confidence: 99%

Evaluation of wound healing potential of pomegranate (Punica granatum) whole fruit extract on skin burn wound in rats (Rattus norvegicus)

et al. 2019

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Objective: This research was conducted to study the wound healing process of whole fruit pomegranate extract ( punica granatum ) standardized with 40% ellagic acid ointment for deep second-degree burn wound of skin in the rat ( Rattus norvegicus ). Materials and Methods: Powder of standardized pomegranate extract (SPE) with 40% ellagic acid was processed to become ointments. Twenty-five male rats, weighed 150–180 gm at 3 months of age, were randomly divided into five groups. After anesthetized, stainless circle plate with 1 cm of diameter in 85°C was contacted firmly toward right gluteal of rat skins for 5 sec in order to create deep second-degree burn wound. Control groups consist of (T0) cream base and (T1) 1% silver sulfadiazine. Treatment groups consist of (T2) 2.5% SPE, (T3) 5% SPE, and (T4) 10% SPE. Histopathological preparation used hematoxylin-eosin stained skin samples. Histological observations were performed using the optics microscope against collagen, the number of polymorphonuclear cell (PMN) infiltration, the degree of angiogenesis, and re-epithelization. The results were statistically compared between groups. Results: Microscopic observation on the wound healing process on the collagen, PMN infiltration, angiogenesis, and re-epithelization showed that topical administration of 10% SPE in burns gives the best result. This is characterized by a high density of collagen with a good arrangement, which is accompanied by a complete and mature epithelium, low number of inflammatory cells, and angiogenesis. This may be caused by the compounds in the pomegranate extract, which have the antioxidant, anti-inflammatory, and anti-bacterial effects. Conclusion: This study reveals that 10% SPE accelerates the healing of deep second-degree burn wound. Thus, pomegranate standardized with 40% ellagic acid is a promising herb for the healing of burn wound of skin.

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“…Specimens were stained using hematoxylin eosin. Collagen scoring, number of polymorphonuclear cells (PMN), angiogenesis, and re-epithelialization were evaluated using microscope CX41 Olympus Japan with 400x magnification (Table 4) (Shakya et al, 2016;Nasiri et al, 2015).…”

Section: Routine Stainingmentioning

confidence: 99%

Efficacy of Transdermal Delivery Nano Ethosomal Gel from Ashitaba Leaves on In-vivo Burn Wound Healing in Albino Rats

Al-Anshori,

Putri,

Ismi

et al. 2023

J. Med. Vet.

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This study aimed to evaluate the in-vivo burn wound healing process in albino rats treated using transdermal delivery nano ethosomal gel from ashitaba leaves. Ethosomal vesicles were formulated using soy lecithin, cholesterol, ethanol, water, and ashitaba leaf nanoparticles using the cold method. A total of 25 male rats were randomly divided into 5 groups, i.e., (C-) treated without nanoparticle extract, (C+) treated using 1% Silver Sulfadiazine®, treatment group treated nano ethosomal gel from ashitaba leaves with the respective doses were (T1) 1%, (T2) 2,5%, and (T3) 5%. Therapy was initiated on day 1 or after being induced by a burn wound for 14 days, twice a day. On the 15th day, a termination was carried out to take skin tissue from burn scars, then histopathology preparations were made with routine staining. Microscopic observations with an optical microscope on collagen, polymorphonuclear cell infiltration, angiogenesis, and re-epithelialization. The T3 group that was treated using 5% nano ethosomal gel showed the best burn wound healing, this may be caused by compounds in ashitaba leaf nanoparticles which have antioxidant, anti-inflammatory, and antibacterial effects, thus the use of transdermal delivery therapy of 5% ashitaba leaf nano ethosomal gel was effective for wound treatment burn on rat skin.

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Bubaline Cholecyst Derived Extracellular Matrix for Reconstruction of Full Thickness Skin Wounds in Rats

Cited by 11 publications

References 39 publications

Effectiveness Of Incision Wound Healing Of Pandanus Amaryllifolius Roxb In Wistar Rats

Effectiveness Of Incision Wound Healing Of Pandanus Amaryllifolius Roxb In Wistar Rats

Evaluation of wound healing potential of pomegranate (Punica granatum) whole fruit extract on skin burn wound in rats (Rattus norvegicus)

Efficacy of Transdermal Delivery Nano Ethosomal Gel from Ashitaba Leaves on In-vivo Burn Wound Healing in Albino Rats

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