2017
DOI: 10.1126/science.aaf4706
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Bug mapping and fitness testing of chemically synthesized chromosome X

Abstract: INTRODUCTION Design and construction of an extensively modified yeast genome is a direct means to interrogate the integrity, comprehensiveness, and accuracy of the knowledge amassed by the yeast community to date. The international synthetic yeast genome project (Sc2.0) aims to build an entirely designer, synthetic Saccharomyces cerevisiae genome. The synthetic genome is designed to increase genome stability and genetic flexibility while maintaining cell fitness near that of the wild type. A major challenge fo… Show more

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Cited by 189 publications
(141 citation statements)
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“…Third, synonymous recoding within an open reading frame is generally well tolerated. We found at least one case during the process of replacing megachunk E, in which the function of MMM1 was impaired as a result of recoding to generate a PCRTag (YLL006W.1F) (9,10). Fourth, deletion of a hypothetical intron within the 5′ UTR of COQ9 led to a transcriptional block ( fig.…”
Section: Synxii Debuggingmentioning
confidence: 92%
“…Third, synonymous recoding within an open reading frame is generally well tolerated. We found at least one case during the process of replacing megachunk E, in which the function of MMM1 was impaired as a result of recoding to generate a PCRTag (YLL006W.1F) (9,10). Fourth, deletion of a hypothetical intron within the 5′ UTR of COQ9 led to a transcriptional block ( fig.…”
Section: Synxii Debuggingmentioning
confidence: 92%
“…The Saccharomyces cerevisiae 2.0 project (Sc2.0), taking a similar de novo approach, aims to modify the yeast genome with a series of densely spaced designer changes. Several synthetic chromosomes have been shown to function in yeast, including the synthetic yeast chromosome arm (synIXR), the entirely synthetic chromosome (synIII) (6,7), and four additional synthetic chromosomes described in this issue (8)(9)(10)(11). For designer genome synthesis projects, precisely matching the physical sequence to the specified design is important for the systematic evaluation of underlying design principles.…”
mentioning
confidence: 99%
“…Recently, two papers, one on the design and synthesis of a chromosome arm and the other about the first completely synthetic chromosome were published [32,34]. Excitingly, seven papers which covering five synthetic chromosomes were published in recent issue of Science, setting another milestone in the journey of eukaryotic genome synthesis [35][36][37][38][39][40][41]. Work on the synthesis of remaining yeast chromosomes is ongoing around the world.…”
mentioning
confidence: 99%
“…The BAG course served as a great teaching vehicle to educate the young students, giving them chance to experience real scientific research and synthetic biology. Up to now, through collaboration, the consortium has ChrII, ChrV, ChrVI, ChrX and ChrXII completely synthesized [36,[38][39][40][41] while several other chromosomes are in progress or near completion. In addition, several tools/technologies have been developed through the execution of Sc2.0 [26,43,44].…”
mentioning
confidence: 99%