“Bugbead”: an artificial microorganism model used as a harmless simulant for pathogenic microorganisms

Kradtap, Supaporn; Wijayawardhana, Charles; Schlueter, Kevin T.; Halsall, H. Brian; Heineman, William R.

doi:10.1016/s0003-2670(01)01152-7

Cited by 16 publications

(12 citation statements)

References 8 publications

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“…The development of magnetoimmunosensors for the detection of pathogen bacteria was firstly described by Kradtap et al [23] and Che et al [24]. Kradtap et al used an artificial microorganism called "Bugbead" as stimulant for the development of microbial sensors without introducing potential experimental hazards or other variables coming in when using real microorganisms [23].…”

Section: Detection Of Viral Bacterial and Antibacterial Agentsmentioning

confidence: 99%

“…Kradtap et al used an artificial microorganism called "Bugbead" as stimulant for the development of microbial sensors without introducing potential experimental hazards or other variables coming in when using real microorganisms [23]. The applicability of these bugbeads in the development of immunosensors was demonstrated by the analysis of extracts containing serotype antigens of Escherichia coli O157 : H7 (E. coli O157 : H7).…”

Section: Detection Of Viral Bacterial and Antibacterial Agentsmentioning

confidence: 99%

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Magnetic Beads‐Based Electrochemical Sensors Applied to the Detection and Quantification of Bioterrorism/Biohazard Agents

2011

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Nowadays, detecting the presence of bioterrorism and biohazard agents in environmental and food samples is of great concern, due to their toxicity, and because many of them are prone to be used in terrorism attacks. The use of functionalized magnetic beads (MBs) in the development of electrochemical immuno-and genosensors has resulted in innovative and powerful detection strategies that may be applied to environmental, food and clinical analysis. This review describes current research on the combination of functionalized MBs with electrochemical detection for the development of magnetobiosensors applied to rapid, sensitive and specific detection of bioterrorism and biohazard agents.

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Section: Detection Of Viral Bacterial and Antibacterial Agentsmentioning

confidence: 99%

Section: Detection Of Viral Bacterial and Antibacterial Agentsmentioning

confidence: 99%

Magnetic Beads‐Based Electrochemical Sensors Applied to the Detection and Quantification of Bioterrorism/Biohazard Agents

2011

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“…[18][19][20][21] Therefore, our group also tried to construct an electrochemical immunoassay system using magnetic microbeads. 22,23 In the previous work based on the chemiluminescent detection, magnetic microbeads coated with agarose gel were used.…”

Section: ·3 Electrochemical Determinationmentioning

confidence: 99%

Sequential Injection Immunoassay for Environmental Measurements

et al. 2011

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“…18 We have applied hydrodynamic voltammetry using a rotating disk electrode (RDE) to the magnetic microbead-based enzyme immunoassay with amperometric detection in a microlitre droplet. [19][20][21][22][23][24] The RDE has a dual purpose in these assays. It can be used effectively to mix the sample solution and at the same time acts as an electrochemical detection device.…”

Section: Introductionmentioning

confidence: 99%

Magnetic microbead-based enzyme immunoassay for ovalbumin using hydrodynamic voltammetry and fluorometric detection

2012

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A paramagnetic microbead-based enzyme immunoassay was demonstrated for detecting ovalbumin (OVA). The immunoassay sandwich was made by attaching a biotinylated antibody to the streptavidin coated beads as a mobile solid phase, capturing antigen, and then exposing the antigen to an antibody conjugated with b-galactosidase. b-Galactosidase converts p-aminophenyl galactopyranoside (PAPG) and fluorescein di-b-D-galactopyranoside (FDG) to p-aminophenol (PAP) and fluorescein, respectively. The current response of PAP generated by the enzymatic reaction of b-galactosidase was detected with hydrodynamic voltammetry in a droplet using a rotating disk electrode (RDE) system. The performance of this electrochemical assay was compared with fluorometric detection of fluorescein produced by the same assay system. The limits of detection for OVA determined by hydrodynamic amperometry and fluorometry were 2.1 nM (43 fmol) and 2.9 nM (58 fmol), respectively. Furthermore, the effects of the conditions commonly found in drinking water supply systems on the OVA assay were also evaluated.

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“Bugbead”: an artificial microorganism model used as a harmless simulant for pathogenic microorganisms

Cited by 16 publications

References 8 publications

Magnetic Beads‐Based Electrochemical Sensors Applied to the Detection and Quantification of Bioterrorism/Biohazard Agents

Magnetic Beads‐Based Electrochemical Sensors Applied to the Detection and Quantification of Bioterrorism/Biohazard Agents

Sequential Injection Immunoassay for Environmental Measurements

Magnetic microbead-based enzyme immunoassay for ovalbumin using hydrodynamic voltammetry and fluorometric detection

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