Bugs and drugs: a systems biology approach to characterising the effect of moxidectin on the horse’s faecal microbiome

Daniels, Simon; Swann, Jonathan R.; Proudman, C. J.

doi:10.1186/s42523-020-00056-2

Cited by 13 publications

(26 citation statements)

References 61 publications

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“…We previously highlighted that susceptible ponies had lower monocyte but higher lymphocyte counts than resistant individuals upon natural strongylid infection 34 . More susceptible individuals also exhibited differential modulation of their faecal microbiota, including enrichment for the Ruminococcus genus 34 , corroborating independent observations of alterations in the gut microbiota composition of infected horses 34 – 37 .…”

Section: Introductionsupporting

confidence: 80%

Integrative biology defines novel biomarkers of resistance to strongylid infection in horses

Sallé

Canlet

Cortet

et al. 2021

Sci Rep

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The widespread failure of anthelmintic drugs against nematodes of veterinary interest requires novel control strategies. Selective treatment of the most susceptible individuals could reduce drug selection pressure but requires appropriate biomarkers of the intrinsic susceptibility potential. To date, this has been missing in livestock species. Here, we selected Welsh ponies with divergent intrinsic susceptibility (measured by their egg excretion levels) to cyathostomin infection and found that their divergence was sustained across a 10-year time window. Using this unique set of individuals, we monitored variations in their blood cell populations, plasma metabolites and faecal microbiota over a grazing season to isolate core differences between their respective responses under worm-free or natural infection conditions. Our analyses identified the concomitant rise in plasma phenylalanine level and faecal Prevotella abundance and the reduction in circulating monocyte counts as biomarkers of the need for drug treatment (egg excretion above 200 eggs/g). This biological signal was replicated in other independent populations. We also unravelled an immunometabolic network encompassing plasma beta-hydroxybutyrate level, short-chain fatty acid producing bacteria and circulating neutrophils that forms the discriminant baseline between susceptible and resistant individuals. Altogether our observations open new perspectives on the susceptibility of equids to strongylid infection and leave scope for both new biomarkers of infection and nutritional intervention.

show abstract

Section: Introductionsupporting

confidence: 80%

Integrative biology defines novel biomarkers of resistance to strongylid infection in horses

Sallé

Canlet

Cortet

et al. 2021

Sci Rep

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show abstract

“…We previously highlighted that susceptible ponies had lower monocyte but higher lymphocyte counts than resistant individuals upon natural strongylid infection 25 . More susceptible individuals also exhibited differential modulation of their faecal microbiota, including enrichment for the Ruminococcus genera 25 , corroborating independent observations of alterations in the gut microbiota composition of infected horses [25][26][27][28] .…”

Section: Introductionsupporting

confidence: 79%

Integrative biology defines novel biomarkers of resistance to strongylid infection in horses

Sallé

Canlet

Cortet

et al. 2021

Preprint

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The widespread failure of anthelmintic drugs against nematodes of veterinary interest requires novel control strategies. Selective treatment of the most susceptible individuals could reduce drug selection pressure but requires appropriate biomarkers of the intrinsic susceptibility potential. To date, this has been missing in livestock species. Here, we selected Welsh ponies with divergent intrinsic susceptibility to cyathostomin infection and found that their potential was sustained across a 10-year time window. Using this unique set of individuals, we monitored variations in their blood cell populations, plasma metabolites and faecal microbiota over a grazing season to isolate core differences between their respective responses under worm-free or natural infection conditions. Our analyses identified the concomitant rise in plasmatic phenylalanine level and faecal Prevotella abundance and the reduction in circulating monocyte counts as biomarkers of the need for drug treatment. This biological signal was replicated in other independent populations. We also unravelled an immunometabolic network encompassing plasmatic beta-hydroxybutyrate level, short-chain fatty acid producing bacteria and circulating neutrophils that forms the discriminant baseline between susceptible and resistant individuals. Altogether our observations open new perspectives on the susceptibility of equids to cyathostomin infection and leave scope for both new biomarkers of infection and nutritional intervention.

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“…Peachey et al ( 25 ) confirmed again that anthelmintic treatment was associated with alteration of the relative abundances of the bacterial community. Daniels et al ( 26 ) observed that anthelmintic treatment increased the relative abundances of Deferribacter spp. and Spirochaetes spp.…”

Section: Introductionmentioning

confidence: 99%

Metagenomic Analysis of Fecal Archaea, Bacteria, Eukaryota, and Virus in Przewalski's Horses Following Anthelmintic Treatment

Yang²,

et al. 2021

Front. Vet. Sci.

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Intestinal microbiota is involved in immune response and metabolism of the host. The frequent use of anthelmintic compounds for parasite expulsion causes disturbance to the equine intestinal microbiota. However, most studies were on the effects of such treatment on the intestinal bacterial microbes; none is on the entire microbial community including archaea and eukaryotic and viral community in equine animals. This study is the first to explore the differences of the microbial community composition and structure in Przewalski's horses prior to and following anthelmintic treatment, and to determine the corresponding changes of their functional attributes based on metagenomic sequencing. Results showed that in archaea, the methanogen of Euryarchaeota was the dominant phylum. Under this phylum, anthelmintic treatment increased the Methanobrevibacter genus and decreased the Methanocorpusculum genus and two other dominant archaea species, Methanocorpusculum labreanum and Methanocorpusculum bavaricum. In bacteria, Firmicutes and Bacteroidetes were the dominant phyla. Anthelmintic treatment increased the genera of Clostridium and Eubacterium and decreased those of Bacteroides and Prevotella and dominant bacteria species. These altered genera were associated with immunity and digestion. In eukaryota, anthelmintic treatment also changed the genera related to digestion and substantially decreased the relative abundances of identified species. In virus, anthelmintic treatment increased the genus of unclassified_d__Viruses and decreased those of unclassified_f__Siphoviridae and unclassified_f__Myoviridae. Most of the identified viral species were classified into phage, which were more sensitive to anthelmintic treatment than other viruses. Furthermore, anthelmintic treatment was found to increase the number of pathogens related to some clinical diseases in horses. The COG and KEGG function analysis showed that the intestinal microbiota of Przewalski's horse mainly participated in the carbohydrate and amino acid metabolism. The anthelmintic treatment did not change their overall function; however, it displaced the population of the functional microbes involved in each function or pathway. These results provide a complete view on the changes caused by anthelmintic treatment in the intestinal microbiota of the Przewalski's horses.

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Bugs and drugs: a systems biology approach to characterising the effect of moxidectin on the horse’s faecal microbiome

Cited by 13 publications

References 61 publications

Integrative biology defines novel biomarkers of resistance to strongylid infection in horses

Integrative biology defines novel biomarkers of resistance to strongylid infection in horses

Integrative biology defines novel biomarkers of resistance to strongylid infection in horses

Metagenomic Analysis of Fecal Archaea, Bacteria, Eukaryota, and Virus in Przewalski's Horses Following Anthelmintic Treatment

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