Burden and Associated Genotype Patterns of High-Risk Human Papilloma Virus Infection and Cervical Cytology Abnormalities among Women in Central India

Gupta, Shipra; Purwar, Shashank; Gupta, Priyal; Halder, Ajay; Gupta, Ayush; Pushpalatha, K; John, Julie Hansa

doi:10.1155/2022/3932110

Cited by 9 publications

(17 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Variations in the proportion of abnormal cytology among HPV cases were reported worldwide [12][13][14][15][16][17]. As compared to other studies from different parts of India as well as worldwide, 5-20% abnormal cytology cases were reported, which correlates well with our study [15][16][17][18].…”

Section: Resultssupporting

confidence: 89%

“…(No. 3B1423/3B1424, 3B Black Bio Biotech India Ltd.) was used for detection and genotyping of Human papilloma virus DNA in clinical samples [12]. This multiplex PCR commercial kit uses fluorescent reporter dye probes specific for detection and genotyping of high-risk HPV 14 genotypes (16,18,31,33,35,39,45,51,52,56,58,59, 66, and 68).…”

Section: Dna Extraction and Hpv Genotype Detectionmentioning

confidence: 99%

See 1 more Smart Citation

Prevalence and Distribution of High-risk Human Papilloma Virus in Cervical Lesions and Asymptomatic Healthy Subjects of Central India

Taraphdar¹,

Hawaldar²,

Sodani³

2023

Act Scie Micro

View full text Add to dashboard Cite

Background: Detection of human papilloma virus (HPV) infection and its genotype distribution are much-needed parameters to judge the risk of cervical cancer among females. However, due to less availability of data on HPV burden in Central part of India makes cervical cancer screening difficult.Methods: A total of 270 participants had been tested for abnormalities in cytology (Pap smear) and real-time PCR was performed for HPV DNA detection. Result:The most common cytological finding among HPV positive patients was found to be of negative for intraepithelial lesion or malignancy NILM (93.7%), followed by atypical squamous cells of undetermined significance (ASCUS) (5.2%), low-grade intraepithelial lesion (LSIL) (0.7%) and high-grade intraepithelial lesion (0.35%) cases. HPV DNA was detected in (8.1%) patients.Highest positivity was seen in the age group of 30-39 years followed by age group of 20-29 years. The most common HPV type in our study was HPV 16 (45.4%) followed by HPV 31 infection (27.2%). Other genotypes of HPV found were HPV18 (13.6%), HPV 51 (2; 9%), HPV 58 (2; 9%), and HPV 39 or 68 * (9%). Conclusion:It is evident from our study that HPV DNA infection was observed with normal cervical cytology. For early detection both Pap smear and HPV DNA genotype detection should be the choice for preventing cervical cancer in women.

show abstract

Section: Resultssupporting

confidence: 89%

Section: Dna Extraction and Hpv Genotype Detectionmentioning

confidence: 99%

Prevalence and Distribution of High-risk Human Papilloma Virus in Cervical Lesions and Asymptomatic Healthy Subjects of Central India

Taraphdar¹,

Hawaldar²,

Sodani³

2023

Act Scie Micro

View full text Add to dashboard Cite

show abstract

“…The current prevalence of abnormal cervical cytology was 21.15% and it is being higher than the reports from the neighboring country Oman (6.92% and 3.5%) [15,22], even from Mexico in 2019 (14.4%) [23] and lower than the reports from Iran (25%) [24]. The current rate was almost nearer to the reports which were from USA (19.6%) and India (19.6%) in 2022 [10,25]. The current rate was lesser than the reports which were published in 2016 by Sign (46.8%) [26] and in 2013 by Elkharashy et al from Egypt (44.44%) [27].…”

Section: Discussionmentioning

confidence: 61%

Exploring the prevalence of Human Papillomavirus (HPV) genotypes in PAP smear samples of women in northern region of United Arab Emirates (UAE): HPV Direct Flow CHIP system-based pilot study

Odeh,

Al-badi,

Karima

et al. 2023

PLoS ONE

View full text Add to dashboard Cite

Objective The aim of this study was to explore the prevalence of low and high-risk HPV genotypes in PAP smear samples of women in northern region of the UAE using HPV direct flow CHIP method. Methods A cross-sectional retrospective study was conducted between September 2021 to April 2022. A total of 104 liquid-based cervical cytology samples were obtained from women aged 20–59 years attending the Gynaecology out-patient department of Thumbay University Hospital and other hospitals of Northern Emirates of UAE, processed for the routine cytological examination to identify and differentiate morphological changes of the PAP smear samples. HPV genotyping was performed using HPV direct flow CHIP method. Results In total, 112 HPV genotypes were detected in 63 women (60.57%) included 18 abnormal cytological and 45 normal epithelial samples. 63 LR and 49 HR HPV genotypes were identified in all the 63 positive samples. Highest rate of infection with multiple LR and HR HPV genotypes were detected in women aged 40–49 years (25.9%) and 20–29 years (23.5%). Infection by HPV6 (13.46%), HPV11 (9.61%), HPV16 (9.61%), HPV62/81 (7.69%) and HPV45 (7.69%) were the most common genotypes. A moderate increase than expected incidence of HPV45 and 62/81 (7.69%) were detected. Co-infection with multiple low and high-risk genotypes is present in 20.2% cases; in that, HPV6 (15.9%) was the most common followed by HPV62/81 (12.7%) and HPV16 (11.11%). The prevalence of HPV18 was found to be 1.6%. Conclusion The genotypes 6, 45, 16, 11, 67, 62/81 were the most common HPV infections in the women between the age group of 21 and 59-years-old. A moderate increase of HPV45, 62/81 and much less prevalence of HPV18 were detected in the study population. 43.27% of the normal epithelia were positive to different low and high-risk HPV genotypes. This finding highlights the importance of molecular genotyping of HPV to emphasize the cervical screening triage.

show abstract

Section: Introductionmentioning

confidence: 99%

“…Anogenital infections are associated with around 40 genotypes of HPV [6]. These strains are categorized into the following three groups based on their oncogenic potency: high-risk, low-risk, and intermediate-risk types [6]. High-risk types include HPV16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 68, 73, and 82, as they are predominantly found in high-grade squamous intraepithelial lesions.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Genotype Patterns Associated With High-Risk Human Papillomavirus in Cervical Lesions

Kothari,

Khullar,

2024

Cureus

View full text Add to dashboard Cite

BackgroundCervical lesions, often linked to high-risk human papillomavirus (HR-HPV) infection, pose a significant public health concern globally. Human HPV is considered the principal etiological factor in the development and transformation of pre-malignant lesions of the cervix leading to cervical carcinoma. The global distribution of HPV genotypes exhibits significant variability, liable to be influenced by the intricate interplay of geographical and biological factors among different HPV types and host immunogenetic elements. Owing to limited research addressing the genotypic distribution of HR-HPV in females with cervical lesions in the western zone of India, this study aims to bridge this gap by providing the prevalence of HR-HPV genotypes in women diagnosed with cervical lesions in this zone. MethodologyThis observational, cross-sectional study was performed in the Laboratory for Genotype Detection in the western zone of India. The study population comprised a total of 215 females in the age range of 18 to 60 years. A thorough examination of clinical specimens was conducted employing molecular techniques for HPV genotyping using TRUPCR® HPV-HR with a 16/18 Genotyping Kit. DNA Extraction was done using 3B Blackbio Biotech India Ltd. as per the standard protocol. The statistical analysis was performed using SPSS version 28 (IBM Corp., Armonk, NY, USA), and continuous variables were compared using Student's t-test. ResultsThe overall prevalence of HR-HPV in women with cervical lesions in the western zone of India was 62.32% (134/215). The most prevalent genotype was HPV16 at 92/134 (68.65%), followed by HPV18 at 33/134 (24.62%) and HPV52 at 9/134 (6.7%). The overall prevalence of single type was 56.71% (76/134). The most prevalent genotype combination after HPV18 + 59 (29.85%) at 40/134 was HPV52 + 39 + 51 (13.43%) at 18/134. HR-HPV infection was found to be significantly (p < 0.05) associated with factors such as having three or more children, having a lower socioeconomic status, residing in rural areas, and being in a premenopausal stage. ConclusionsThis study focused on assessing the prevalence of the genotypes associated with HR-HPV infection, providing valuable insights into the epidemiology of cervical lesions in the western zone of India. The findings revealed high-risk genotype HPV16 to be the most prevalent type, followed by HPV18 and HPV52. The most prevalent genotype combinations were HPV18 + 59 and HPV52 + 39 + 51. The results of the study would potentiate the wealth of epidemiological data related to HPV infection in cervical lesions and could be employed for guiding future interventions and preventive strategies through orientation programs.

show abstract

Burden and Associated Genotype Patterns of High-Risk Human Papilloma Virus Infection and Cervical Cytology Abnormalities among Women in Central India

Cited by 9 publications

References 16 publications

Prevalence and Distribution of High-risk Human Papilloma Virus in Cervical Lesions and Asymptomatic Healthy Subjects of Central India

Prevalence and Distribution of High-risk Human Papilloma Virus in Cervical Lesions and Asymptomatic Healthy Subjects of Central India

Exploring the prevalence of Human Papillomavirus (HPV) genotypes in PAP smear samples of women in northern region of United Arab Emirates (UAE): HPV Direct Flow CHIP system-based pilot study

Prevalence of Genotype Patterns Associated With High-Risk Human Papillomavirus in Cervical Lesions

Contact Info

Product

Resources

About