Burn Patients wounds Infected With Metallo-Beta-Lactamase-Producing Pseudomonas aeruginosa: Multidrug Resistant Strains

Anvarinejad, Mojtaba; Japoni, Aziz; Rafaatpour, Noroddin; Mardaneh, Jalal; Abbasi, Parvin; Shahidi, Maneli Amin; Dehyadegari, Mohammad Ali; Alipour, Ebrahim

doi:10.5812/atr.18182

Cited by 34 publications

(37 citation statements)

References 22 publications

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“…MBL-producing P. aeruginosa strains have now emerged in our burn patients. MBLs have also been reported in other parts of Asia, Europe, North America, South America, Australia, Japan, and Iran (13-22). The prevalence of bla IMP genes in MBL-producing P. aeruginosa isolates from burn wounds in Tehran has been reported at 56.25% (23).…”

Section: Discussionmentioning

confidence: 90%

Emerging Carbapenem-Resistant Pseudomonas aeruginosa Isolates Carrying blaIMP Among Burn Patients in Isfahan, Iran

et al. 2016

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BackgroundMetallo-β-lactamase (MBL)-producing Pseudomonas aeruginosa is a significant pathogen in burn patients.ObjectivesThe aim of this study was to determine the prevalence of carbapenem-resistant P. aeruginosa isolates, including those resistant to imipenemase (IMP), in a burn unit in Isfahan, Iran.Patients and MethodsOne hundred and fifty P. aeruginosa isolates from burn patients were tested for antibiotic susceptibility by the disc diffusion method in accordance with CLSI guidelines. Production of MBL was identified with the EDTA disk method. DNA was purified from the MBL-positive isolates, and detection of the blaIMP gene was performed with PCR.ResultsFifty-seven out of 150 (38%) isolates were multi-drug resistant (MDR), and 93 (62%) were extensively-drug resistant (XDR). Among all isolates, the resistance rate to ciprofloxacin, tobramycin, imipenem, meropenem, amikacin, ceftazidime, and cefepime was higher than 90%, while the resistance rates to piperacillin/tazobactam and aztreonam were 70.7% and 86%, respectively. Colistin and polymyxin B remained the most effective studied antibiotics. All of the imipenem-resistant P. aeruginosa isolates were MBL-positive, and 107 out of 144 (74.3%) of the MBL isolates were positive for the blaIMP gene.ConclusionsThe results of this study show that the rate of P. aeruginosa-caused burn wound infections was very high, and many of the isolates were resistant to three or more classes of antimicrobials. Such extensive resistance to antimicrobial classes is important because few treatment options remain for patients with burn wound infections. blaIMP-producing P. aeruginosa isolates are a rising threat in burn-care units, and should be controlled by conducting infection-control assessments.

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Section: Discussionmentioning

confidence: 90%

Emerging Carbapenem-Resistant Pseudomonas aeruginosa Isolates Carrying blaIMP Among Burn Patients in Isfahan, Iran

et al. 2016

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“…In patients with serious infections, the antibiotic therapy may have to be initiated empirically to prevent systemic invasion by infecting bacteria in a formerly debilitated patient while awaiting microbiology laboratory results (17).…”

Section: Discussionmentioning

confidence: 99%

Diabetic Foot Infections: Antibiotic Susceptibility Patterns and Determination of Antibiotic Cross-Resistance in Clinical Isolates of Enterococcus Species During 2012 - 2014 in Shiraz, Iran

Anvarinejad

Pouladfar

Japoni

et al. 2016

Arch Pediatr Infect Dis

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“…Simple and suitable tests are needed to identify MBLproducing isolates that is a crucial step to monitor these emerging resistance. Suppression of enzyme via EDTA is an efficient method used to differentiate MBL mechanisms from other β-lactamases (9,17,26) and also PCR method was valuable to determine MBL-producing isolates (13). In the current study, from 278 imipenem-resistant P. aeruginosa and 187 imipenem-resistant A. baumannii isolates, 178 and 85 isolates were MBL-producers, respectively.…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and Molecular Detection of Metallo-Beta-Lactamase Genes Among Imipenem Resistant Pseudomonas aeruginosa and Acinetobacter baumannii Strains Isolated From Patients with Burn Injuries

Tarashi

Goudarzi

Erfanimanesh

et al. 2016

Arch Clin Infect Dis

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Background: Pseudomonas aeruginosa and Acinetobacter baumannii are the common causes of nosocomial infections especially among patients with burn injuries. Objectives: The current study aimed to determine the frequency of blaIMP, blaVIM, blaDIM, blaAIM, blaGIM and blaNDM genes among P. aeruginosa and A. baumannii strains isolated from patients with burn injuries hospitalized in Shahid Motahari hospital, Tehran, Iran. Methods:The current cross-sectional study evaluated a total of 309 nonduplicate isolates of P. aeruginosa and 189 isolates of A. baumannii collected from different clinical samples of patients with burn injuries in Shahid Motahari hospital in Tehran, Iran, from 2012 to 2015. Antibiotic susceptibility tests were conducted by Kirby-Bauer disc diffusion and broth microdilution methods according to the clinical and laboratory standards institute (CLSI) guidelines. The frequency of metallo-beta-lactamase (MBL) producers was evaluated by the combination disk diffusion test (CDDT). The blaIMP, blaVIM, blaDIM, blaAIM, blaGIM and blaNDM genes were detected by polymerase chain reaction (PCR) and sequencing techniques. Results: The most effective agent against the studied isolates was colistin. By CDDT, it was found that among 278 imipenem resistant P. aeruginosa strains, 178(64.02%) were MBL producers. The blaIMP-1 and blaVIM-1 genes were detected in 30(16.8%) and 52(29.2%) of P. aeruginosa isolates, respectively. Result of 187 imipenem resistance A. baumannii strains showed that 85(45.4%) were MBL producers. The blaOXA-51, blaIMP-1 and blaVIM-1 genes were detected in 187(100%), 10(5.3%) and 34(18.18%) of A. baumannii isolates, respectively. Conclusions: The high prevalence of MBLs-producing P. aeruginosa and A. baumannii strains in the study were one of the major concerns.

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Burn Patients wounds Infected With Metallo-Beta-Lactamase-Producing Pseudomonas aeruginosa: Multidrug Resistant Strains

Cited by 34 publications

References 22 publications

Emerging Carbapenem-Resistant Pseudomonas aeruginosa Isolates Carrying blaIMP Among Burn Patients in Isfahan, Iran

Emerging Carbapenem-Resistant Pseudomonas aeruginosa Isolates Carrying blaIMP Among Burn Patients in Isfahan, Iran

Diabetic Foot Infections: Antibiotic Susceptibility Patterns and Determination of Antibiotic Cross-Resistance in Clinical Isolates of Enterococcus Species During 2012 - 2014 in Shiraz, Iran

Phenotypic and Molecular Detection of Metallo-Beta-Lactamase Genes Among Imipenem Resistant Pseudomonas aeruginosa and Acinetobacter baumannii Strains Isolated From Patients with Burn Injuries

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