Bystander Effects Induced by Medium From Irradiated Cells: Similar Transcriptome Responses in Irradiated and Bystander K562 Cells

Herok, Robert; Konopacka, Maria; Polańska, Joanna; Świerniak, Andrzej; Rogoliński, Jacek; Jaksik, Roman; Hancock, Ronald; Rzeszowska-Wolny, Joanna

doi:10.1016/j.ijrobp.2009.11.033

Cited by 44 publications

(30 citation statements)

References 51 publications

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“…NF κB expression regulated PTGS2 (cyclooxygenase 2), IL8 and BCL2A1 genes, responded similarly in bystander and irradiated cells [46]. No differences in the transcrip tomes of cells grown in medium from X irradiated cells or directly irradiated were seen [47]. From tran script patterns of cells grown in culture medium from irradiated cells was concluded that X irradiated and bystander cells had similar changes in most functional pathways [48].…”

Section: Discussionmentioning

confidence: 99%

Differential regulation of MicroRNA expression in irradiated and bystander cells

Chaudhry

Omaruddin

2012

Mol Biol

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The ionizing radiation (IR) induces a variety of biological effects in irradiated cells. Additionally, the irradiated cells communicate with unirradiated cells and induce changes in them through a phenomenon termed as the bystander effect. The nature of the bystander effect signal and how it impacts unirradiated cells remains to be discovered. Examination of molecular changes in bystander cells due to signals from irradiated cells could lead to the identification of the pathways underlying the bystander effect. To gain insight into the molecular pathways affected by the transmission of signal from irradiated cells to bystander cells, we moni tored the microRNA (miRNA) transcriptional changes. miRNAs control gene expression at the posttran scriptional level. In previous studies from our laboratory the modulation of miRNA in irradiated human cells were identified. In the present work human lymphoblasts TK6 cells in a medium exchanged bystander effect model system were used to analyze miRNA expression alterations by employing the real time RT PCR tech nology. The relative expression of several miRNAs involved in RAS, c MYC and BCL2 gene regulation were examined. The let 7 family of miRNAs was upregulated in irradiated cells but most of these miRNAs remained repressed in bystander cells. The miR 17 3p, miR 19b, and miR-18a were upregulated in irradi ated cells but were repressed in the bystander cells. The miR 17 5p, miR 142 3p, rniR 142 5p, and miR 19a were induced only for a short time in bystander cells. The miR 15a, miR 16, miR 143, miR 145, miR 155, and miR21 were upregulated in irradiated TK6 cells. While the expression of miR 15a, miR 16, miR 155, and miR 21 was repressed, the miR 143 and miR 145 expression was induced in bystander cells. These results indicate the involvement of miRNA modulation in irradiated and bystander cells.

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Section: Discussionmentioning

confidence: 99%

Differential regulation of MicroRNA expression in irradiated and bystander cells

Chaudhry

Omaruddin

2012

Mol Biol

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“…46 No differences in the transcriptomes of cells grown in medium from X-irradiated cells or directly irradiated were seen. 48 The transcript profiles of cells grown in culture medium from irradiated cells and irradiated cells concluded that X-irradiation and bystander effects induce similar changes of transcript profiles in most functional pathways. 49 Cells exposed to ionizing radiation release factors that induce DNA damage, chromosomal instability, apoptosis, alterations in gene expression, and changes in the proliferation rate of neighboring unexposed cells.…”

Section: Chaudhrymentioning

confidence: 97%

Small Nucleolar RNA Host Genes and Long Non-Coding RNA Responses in Directly Irradiated and Bystander Cells

Chaudhry

2014

Cancer Biotherapy and Radiopharmaceuticals

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The irradiated cells communicate with unirradiated cells and induce changes in them through a phenomenon known as the bystander effect. The nature of the bystander signal and how it impacts unirradiated cells remains to be discovered. Examination of molecular changes could lead to the identification of pathways underlying the bystander effect. Apart from microRNAs, little is known about the regulation of other non-coding RNAs (ncRNA) in irradiated or bystander cells. In this study we monitored the transcriptional changes of several small nucleolar RNAs (snoRNAs) host genes and long non-coding RNAs (lncRNAs) that are known to participate in a variety of cellular functions, in irradiated and bystander cells to gain insight into the molecular pathways affected in these cells. We used human lymphoblasts TK6 cells in a medium exchanged bystander effect model system to examine ncRNA expression alterations. The snoRNA host genes SNHG1 and SNHG4 were upregulated in irradiated TK6 cells but were repressed in bystander cells. The SNHG5 and SNHG11 were downregulated in irradiated and bystander cells and the expression levels of these ncRNA were significantly lower in bystander cells. The lncRNA MALAT1, MATR3, SRA1, and SOX2OT were induced in irradiated TK6 cells and their expression levels were repressed in bystander cells. The lncRNA RMST was induced in both irradiated and bystander cells. Taken together, these results indicate that expression levels of ncRNA are modulated in irradiated and bystander cells and these transcriptional changes could be associated with the bystander effect.

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“…The SF can exceed unity when conditions in the exposed flasks are more favourable to colony formation than those in the control flasks, which can occur as a result of adaptive responses to the exposure [5]. Herok et al [6] describe how a stress response induced by a toxin stimulates adaptation. The toxin can be radiation, a chemical agent or a change in physical conditions, such as a change in the growth medium composition.…”

Section: Introductionmentioning

confidence: 99%

Hidden stressors in the clonogenic assay used in radiobiology experiments

Potter

Suchowerska

Rizvi

et al. 2011

Australas Phys Eng Sci Med

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While clonogenic assays are extensively used in radiobiology, there is no widely accepted procedure for choosing the composition of the cell culture media. Cell line suppliers recommend a specific culture medium for each cell line, however a researcher will frequently customize this aspect of the protocol by supplementing the recommended support medium with additives. For example, many researchers add antibiotics, in order to avoid contamination of cells and the consequent loss of data, with little discussion of the influence of the antibiotics on the clonogenic survival of the cells. It is assumed that the effect of any variables in the growth medium on cell survival is taken into consideration by comparing the survival fraction relative to that of controls grown under the same conditions. In the search for better cancer treatment, the effect of various stressors on clonogenic cell survival is under investigation. This study seeks to identify and test potential stressors commonly introduced into the cell culture medium, which may confound the response to radiation.

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Bystander Effects Induced by Medium From Irradiated Cells: Similar Transcriptome Responses in Irradiated and Bystander K562 Cells

Cited by 44 publications

References 51 publications

Differential regulation of MicroRNA expression in irradiated and bystander cells

Differential regulation of MicroRNA expression in irradiated and bystander cells

Small Nucleolar RNA Host Genes and Long Non-Coding RNA Responses in Directly Irradiated and Bystander Cells

Hidden stressors in the clonogenic assay used in radiobiology experiments

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