c-Jun regulates adipocyte differentiation via the KLF15-mediated mode

Lee, Da Som; Choi, Hong-Seok; Han, Baek Soo; Kim, Won Kon; Lee, Sang Chul; Oh, Kyoung-Jin; Bae, Kwang‐Hee

doi:10.1016/j.bbrc.2015.12.035

Cited by 32 publications

(19 citation statements)

References 32 publications

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“…Furthermore, different gene silencing techniques such as siRNA and shRNA, together with different transfection procedures (adenovirus, lentivirus transfection, and plasmid electroporation) have been applied to study the function of different genes associated with adipogenesis in 3T3-L1 cells. In particular, inflammatory pathways, adipokine synthesis, and secretion of study enzyme’s function have been investigated through gene silencing in adipocytes [ 43 , 44 , 45 , 46 , 47 ]. These cells have also been useful for deciphering the biological role of several miRNAs, such as miRNA-195a, which plays an essential role in various cellular processes including proliferation and differentiation [ 48 ].…”

Section: Animal Cell Modelsmentioning

confidence: 99%

Cell Models and Their Application for Studying Adipogenic Differentiation in Relation to Obesity: A Review

Ruiz‐Ojeda

Rupérez

Gómez-Llorente

et al. 2016

IJMS

302

221

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Over the last several years, the increasing prevalence of obesity has favored an intense study of adipose tissue biology and the precise mechanisms involved in adipocyte differentiation and adipogenesis. Adipocyte commitment and differentiation are complex processes, which can be investigated thanks to the development of diverse in vitro cell models and molecular biology techniques that allow for a better understanding of adipogenesis and adipocyte dysfunction associated with obesity. The aim of the present work was to update the different animal and human cell culture models available for studying the in vitro adipogenic differentiation process related to obesity and its co-morbidities. The main characteristics, new protocols, and applications of the cell models used to study the adipogenesis in the last five years have been extensively revised. Moreover, we depict co-cultures and three-dimensional cultures, given their utility to understand the connections between adipocytes and their surrounding cells in adipose tissue.

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Section: Animal Cell Modelsmentioning

confidence: 99%

Cell Models and Their Application for Studying Adipogenic Differentiation in Relation to Obesity: A Review

Ruiz‐Ojeda

Rupérez

Gómez-Llorente

et al. 2016

IJMS

302

221

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“…KLF15 is directly induced by the glucocorticoid receptor, and glucocorticoid response element sites have been located within the first intron of the Klf15 gene (100) . c-Jun interrupts the glucocorticoid-mediated induction of KLF15 by occupying an intronic region near the glucocorticoid response element site of the Klf15 gene (102) . Thus, the browning of WAT is promoted by KLF11 and KLF15.…”

Section: Klf Biology In Organs Of Metabolic Interestmentioning

confidence: 99%

Krüppel-Like Factors

Pollak

Hoffman

Goldberg

et al. 2018

JACC: Basic to Translational Science

102

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SummaryKrüppel-like factors (KLFs) are deoxyribonucleic acid–binding transcriptional factors that regulate various pathways that control metabolism and other cellular mechanisms. Various KLF isoforms have been associated with cellular, organ, or systemic metabolism. Altered expression or activation of KLFs has been linked to metabolic abnormalities, such as obesity and diabetes, as well as with heart failure. This review article summarizes the metabolic functions of KLFs, as well as the networks of different KLF isoforms that jointly regulate metabolism in health and disease.

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“…The glucocorticoid receptor activated KLF15 expression during adipogenesis of 3T3‐L1 (Sasse et al, ). The transcription factor c‐JUN was able to bind to Klf15 promoter near the glucocorticoid response element inhibiting the glucocorticoid stimulated adipogenesis (Lee et al, ), suggesting that a new branch of the transcriptional circuit might be targeting the expression of Pparg2 .…”

Section: Adipogenic Factorsmentioning

confidence: 99%

A cellular perspective of adipogenesis transcriptional regulation

Kuri‐Harcuch

Velez-delValle

Vazquez-Sandoval

et al. 2018

Journal Cellular Physiology

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Adipose cells store lipids in the cytoplasm and signal systemically through secretion of adipokines and other molecules that regulate body energy metabolism. Differentiation of fat cells and its regulation has been the focus of extensive research since the early 1970s. In this review, we had attempted to examine the research bearing on the control of adipose cell differentiation, some of it dating back to the early days when Howard Green and his group described the preadipocyte cell lines 3T3-L1 and 3T3-F442A during 1974-1975. We also concentrated our attention on research published during the last few years, emphasizing data described on transcription factors that regulate adipose differentiation, outside of those that were reported earlier as part of the canonical adipogenic transcriptional cascade, which has been the subject of ample reviews by several groups of researchers. We focused on the studies carried out with the two preadipocyte cell culture models, the 3T3-L1 and 3T3-F442A cells that have provided essential data on adipose biology.

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c-Jun regulates adipocyte differentiation via the KLF15-mediated mode

Cited by 32 publications

References 32 publications

Cell Models and Their Application for Studying Adipogenic Differentiation in Relation to Obesity: A Review

Cell Models and Their Application for Studying Adipogenic Differentiation in Relation to Obesity: A Review

Krüppel-Like Factors

A cellular perspective of adipogenesis transcriptional regulation

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