C-kit receptor in the human vas deferens: distinction of mast cells, interstitial cells and interepithelial cells

Metzger, Roman; Rolle, Udo; Fiegel, Henning C.; Franke, Folker E.; Muenstedt, Karsten; Till, Holger

doi:10.1530/rep-07-0346

Cited by 13 publications

(10 citation statements)

References 36 publications

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“…Finally, our data confirm and expand the set of negative excretory duct system IHC markers to include PSA, PSMA, WT1, calretinin, OCT4, SALL4, and CD117. 5,17,18,21,22 TV is a mesothelial-derived cell layer that forms the outer surface of the testis and lines the potential space of the scrotal sac. 2 Our data indicate that TV expresses CK7, WT1, and calretinin, which is concordant with previously published data and supports its mesothelial origin.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Immunophenotypic Characterization of Adult and Fetal Testes, the Excretory Duct System, and Testicular and Epididymal Appendages

Magers¹,

Udager²,

Chinnaiyan

et al. 2016

Applied Immunohistochemistry &Amp; Molecular Morphology

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The immunophenotype of a normal testis and the excretory duct system has not been studied comprehensively in fetal and adult patients without testicular disease or hormonal manipulation so far. In addition, testicular (TA) and epididymal (EA) appendages are frequent paratesticular structures without previously reported comprehensive immunophenotypic studies. Immunohistochemistry for multiple markers, including the androgen receptor (AR), the estrogen receptor (ER), the progesterone receptor (PR), the prostate-specific antigen, the prostate-specific membrane antigen, PAX8, WT1, calretinin, CK7, CK20, OCT4, SALL4, and CD117, was performed on full sections of testicular/paratesticular tissue from a large cohort of adult and fetal autopsy patients. In contrast to adult germ cells (GC), fetal GC strongly express OCT4 and CD117, although the expression of these proteins is lost in the early postnatal period; SALL4, in contrast, is expressed in both fetal and adult GC, with only weak and focal expression in adult patients. Fetal Sertoli cells (SC) express WT1 and calretinin strongly and diffusely, in contrast to adult SC. Both fetal and adult excretory duct systems express CK7 and PAX8 with frequent AR coexpression, and all 3 main segments of the excretory duct system (ductuli efferentes, epididymis, and vas deferens) have unique immunophenotypes. The rete testis also has a unique immunohistochemical expression pattern, which includes strong expression of CK7, PAX8, WT1, calretinin, and AR. Finally, of the adult autopsy patients examined, 80% had a TA, and 60% had an EA; these paratesticular structures occurred at stereotypical locations, demonstrated reproducible morphologic features, and had a unique immunophenotype relative to other studied structures, with strong CK7, PAX8, WT1, AR, ER, and PR coexpression. The testis and the paratestis may be involved by diverse neoplastic and non-neoplastic processes, and knowledge of the immunophenotypic expression spectrum of these tissues may aid in clinical diagnosis and advance our understanding of the pathogenesis of both oncologic and nononcologic disease processes.

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Section: Discussionmentioning

confidence: 99%

Comprehensive Immunophenotypic Characterization of Adult and Fetal Testes, the Excretory Duct System, and Testicular and Epididymal Appendages

Magers¹,

Udager²,

Chinnaiyan

et al. 2016

Applied Immunohistochemistry &Amp; Molecular Morphology

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“…We initially used c‐kit immunohistochemistry similar to previous studies 9,10,12 but we subsequently adopted a double immunofluorescence technique to better distinguish mast cells from ICC. Problems with autofluorescence were overcome by reducing primary antibody concentrations and incubation periods and the preferential selection of surgical specimens; the use of Sudan black B was unhelpful 22 . No obvious differences between monoclonal and polyclonal c‐kit antibody immunolabeling emerged.…”

Section: Discussionmentioning

confidence: 99%

Interstitial cells of Cajal are present in human extrahepatic bile ducts

Ahmadi

Nicholson

Gould

et al. 2010

J of Gastro and Hepatol

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“…8 C-kit immune positive-stained cells have also been demonstrated in the urinary bladder, urethra, prostatic stroma, vas deferens, corpus cavernosum and spermatogonia. [10][11][12][13][14][15] We investigate the quantitative and ultrastructural changes of ICC UPJ obstruction using light and electron microscopy.…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical and electron microscopic examination of Cajal cells in ureteropelvic junction obstruction

Eken

Erdogan

Kuyucu

et al. 2013

CUAJ

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E311Cite as: Can Urol Assoc J 2013;7(5-6):e311-6. http://dx.doi.org/10.5489/cuaj.11293 Published online May 13, 2013 (early released September 10, 2012). AbstractObjective: We examine the ultrastructural configurations of Cajal cells by electron microscopy, as well as the quantitative changes occurring in Cajal cells by light microscopy. Methods: In total, 35 patients with ureteropelvic junction (UPJ) obstruction and 7 patients without obstruction were compared immunohistochemically with c-kit (CD117) to quantify the number of cells. On electron microscopic examination, 7 patients with UPJ obstruction and 3 patients without obstruction were compared to evaluate the changes which occurred in the ultrastructural configuration of the Cajal cells. Results: On light microscopic examination, it was determined that the Cajal cells, which demonstrate c-kit (CD117) immunoreactive character, were located near the circular muscle layer and parallel to the muscle cells. The number of Cajal cells in the control group was significantly increased compared to the number of cells in patients with UPJ obstruction (p < 0.001). On electron microscopic examination, the number of interstitial cells was also higher in the control group. A decrease in the number of the caveolae in these cells was seen in the group with UPJ obstruction compared to the control group. Conclusion: In UPJ obstruction, a decrease in the number of Cajal cells, as well as the changes in the morphologic structure of the Cajal cells, indicates that these cells have a role in the pacemaker system and are associated with ureteral peristalsis.

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C-kit receptor in the human vas deferens: distinction of mast cells, interstitial cells and interepithelial cells

Cited by 13 publications

References 36 publications

Comprehensive Immunophenotypic Characterization of Adult and Fetal Testes, the Excretory Duct System, and Testicular and Epididymal Appendages

Comprehensive Immunophenotypic Characterization of Adult and Fetal Testes, the Excretory Duct System, and Testicular and Epididymal Appendages

Interstitial cells of Cajal are present in human extrahepatic bile ducts

Immunohistochemical and electron microscopic examination of Cajal cells in ureteropelvic junction obstruction

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