c-Myc oncogene expression in selected odontogenic cysts and tumors: An immunohistochemical study

Abstract

Aim:To investigate the role of c-Myc oncogene in selected odontogenic cysts and tumors.Materials and Methods:Ten cases each of ameloblastoma, adenomatoid odontogenic tumor (AOT), odontogenic keratocyst (OKC), dentigerous cyst, and radicular cyst were selected and primary monoclonal mouse anti-human c-Myc antibody was used in a dilution of 1: 50. Statistical Analysis was performed using Mann Whitney U test.Results:80% positivity was observed in ameloblastoma, AOT and OKC; 50% positivity in radicular cyst and 20% positivity in dentigerous cyst. Comparison of c-Myc expression between ameloblastoma and AOT did not reveal significant results. Similarly, no statistical significance was observed when results of OKC were compared with ameloblastoma and AOT. In contrast, significant differences were seen on comparison of dentigerous cyst with ameloblastoma and AOT and radicular cyst with AOT.Conclusion:From the above data we conclude that (1) Ameloblastoma and AOT have similar proliferative potential and their biologic behavior cannot possibly be attributed to it. (2) OKC has an intrinsic growth potential which is absent in other cysts and reinforces its classification as keratocystic odontogenic tumor.

Elevated hydrostatic pressure promotes ameloblastoma cell invasion through upregulation of MMP‐2 and MMP‐9 expression via Wnt/β‐catenin signalling

“…Data are shown as the mean ± SD from three independent experiments. *P < 0.05 Jun 22 and c-Myc, 23 were detected in ameloblastoma tissues. Their expression was upregulated under elevated HP at both mRNA and protein levels.…”

“…In hTERT + ‐AM cells, HP‐induced activation of β‐catenin signalling was blocked by DKK1, suggesting that this process depends on the Wnt co‐receptors LRP5/6. Wnt/β‐catenin signalling downstream targets, including LEF‐1, cyclin D1, c‐Jun and c‐Myc, were detected in ameloblastoma tissues. Their expression was upregulated under elevated HP at both mRNA and protein levels.…”

Oncogenic signalling pathways in benign odontogenic cysts and tumours

“…While ameloblastoma is locally aggressive and can perforate the cortical bone plate and infiltrate soft tissues, AOT is encapsulated with limited growth potential and very low tendency of recurrence. Notably, both the odontogenic tumours show expression of the MAPK transcriptional targets, cyclin-D1 and c-Myc, which regulate cell proliferation [21][22][23][24][25]. In colorectal cancer, it has been shown that BRAFV600E and KRASG12V oncogenes have a different impact on morphology and invasive phenotype [26].…”

Lysine-specific demethylase 1 promotes tumorigenesis and predicts prognosis in gallbladder cancer

“…The C-myc proto-oncogene, which was first detected in Burkitt's lymphoma, is over-expressed in most tumors [ 6 , 7 ], including oral squamous cell carcinoma (OSCC) [ 8 ]. As an oncogenic transcription factor, the C-myc protein recognizes the E-box recognition site and several related, non-canonical sequences in the promoter regions of target genes [ 9 ].…”