2010
DOI: 10.1074/jbc.m109.044495
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C-reactive Protein Exists in an NaCl Concentration-dependent Pentamer-Decamer Equilibrium in Physiological Buffer

Abstract: C-reactive protein (CRP) is an acute phase protein of the pentraxin family that binds ligands in a Ca2؉ -dependent manner, and activates complement. Knowledge of its oligomeric state in solution and at surfaces is essential for functional studies. Analytical ultracentrifugation showed that CRP in 2 mM Ca 2؉ exhibits a rapid pentamer-decamer equilibrium. The proportion of decamer decreased with an increase in NaCl concentration. The sedimentation coefficients s 20,w 0 of pentameric and decameric CRP were 6.4 S … Show more

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Cited by 38 publications
(50 citation statements)
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“…1B). This result is different from hCRP, which shows pentamer-decamer equilibrium by AUC (Okemefuna et al, 2010), and the discrepancy might be due to species differences. The high-resolution 3D structures of Dare-PTX (1.7 Å) and Dare-PTX-Ca (1.65 Å) also show that Dare-PTX forms trimers, which is in agreement with the results of analytical ultracentrifugation and gelfiltration.…”
Section: Dare-ptx Is a Trimercontrasting
confidence: 39%
“…1B). This result is different from hCRP, which shows pentamer-decamer equilibrium by AUC (Okemefuna et al, 2010), and the discrepancy might be due to species differences. The high-resolution 3D structures of Dare-PTX (1.7 Å) and Dare-PTX-Ca (1.65 Å) also show that Dare-PTX forms trimers, which is in agreement with the results of analytical ultracentrifugation and gelfiltration.…”
Section: Dare-ptx Is a Trimercontrasting
confidence: 39%
“…These basic surfaces may interact with acidic surfaces on CRP. However, experimental studies with CRP are complicated by the ease with which CRP becomes denatured, for example, by the removal of Ca 2ϩ , given that Ca 2ϩ binds to CRP with a moderate dissociation constant (K D ) of 60 M (13,19). The abnormally modified forms of CRP created by Ca 2ϩ removal have functional properties that are very different from the highly stable CRP pentamer.…”
Section: Factor H (Fh)mentioning
confidence: 99%
“…In addition, FH concentrations were close to either 1 or 0.01 M, indicating two very different FH-CRP affinities. Here, we worked under conditions at which CRP forms native stable pentamers (13). We show that FH binds to native CRP at acute phase concentration levels in both the solution and surface phases with Ca 2ϩ present and in a NaCl concentration-dependent manner.…”
Section: Factor H (Fh)mentioning
confidence: 99%
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