2009
DOI: 10.1002/jbm.a.32638
|View full text |Cite
|
Sign up to set email alerts
|

C3, C5, and factor B bind to chitosan without complement activation

Abstract: Chitosan is a polycationic and biocompatible polysaccharide composed of glucosamine and N-acetyl glucosamine that is chemotactic for neutrophils and stimulates wound repair through mechanisms that remain unclear. It was previously shown that chitosan depletes complement proteins from plasma, suggesting that chitosan activates complement. Complement activation leads to cleavage of C5 to produce C5a, a neutrophil chemotactic factor. Here, we tested the hypothesis that chitosan generates C5a in human whole blood,… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1

Citation Types

1
13
0

Year Published

2012
2012
2021
2021

Publication Types

Select...
4
4

Relationship

0
8

Authors

Journals

citations
Cited by 26 publications
(14 citation statements)
references
References 54 publications
1
13
0
Order By: Relevance
“…They showed that increasing the molecular weight of chitosan led to decreased complement activation, reaching negligible levels. (Bertholon et al, 2006) Marchand et al also concluded that chitosan is a non-activating biomaterial; in spite of binding anionic plasma and serum proteins like C3, it does not led to complement activation (Marchand et al, 2010). As the long systemic circulation half-life of the nanoparticles is dependent on their ability to avoid the capture by macrophages of the MPS, the development of 'stealth' nanoparticles is highly desirable.…”
Section: Discussionmentioning
confidence: 99%
“…They showed that increasing the molecular weight of chitosan led to decreased complement activation, reaching negligible levels. (Bertholon et al, 2006) Marchand et al also concluded that chitosan is a non-activating biomaterial; in spite of binding anionic plasma and serum proteins like C3, it does not led to complement activation (Marchand et al, 2010). As the long systemic circulation half-life of the nanoparticles is dependent on their ability to avoid the capture by macrophages of the MPS, the development of 'stealth' nanoparticles is highly desirable.…”
Section: Discussionmentioning
confidence: 99%
“…Considering polycationic materials, such as poly-L-Lysine and chitosan can induce elevated levels of sTCC, Bb, C3a, C5a, and leukocyte CD116 in blood (Benesch and Tengvall, 2002), we used alginate to make microspheres which is a kind of negatively charged biomaterial and has low affinities to plasma proteins such as albumin and C3 (Marchand et al, 2010;Suzukia et al, 2000). The real behavior of C3A cells in the fluidized bed bioreactor was monitored for 24 h, although 6 h is corresponded to a classical session treatment.…”
Section: Discussionmentioning
confidence: 99%
“…It is however, important to note that the results obtained about the complement activation were based on binding and depletion assays. This complement depletion can equally be explained by assuming a tight binding to the chitosan surface without activation [37].…”
Section: Discussionmentioning
confidence: 99%