C3AR1 mRNA as a Potential Therapeutic Target Associates With Clinical Outcomes and Tumor Microenvironment in Osteosarcoma

Zou, Tiannan; Liu, Weibing; Wang, Zeyu; Chen, Jiayu; Lü, Sheng; Huang, Kun; Li, Weichao

doi:10.3389/fmed.2021.642615

Cited by 13 publications

(11 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The overexpression of C3AR1 mRNA was confirmed to inhibit the proliferation, migration, and invasion of OS cells and induce apoptosis. It may be a promising therapeutic target for OS, and it may be related to prognosis and the tumor microenvironment ( 29 ). M6A regulators (i.e., FTO and IGF2BP2) may play an important role in the metastasis of OS, which is of great value to the prognosis and treatment strategy for OS patients ( 30 ).…”

Section: Discussionmentioning

confidence: 99%

Bioinformatics analysis of lncRNAs in the occurrence and development of osteosarcoma

Liu¹,

Zong²,

Sun³

et al. 2022

Transl Pediatr

View full text Add to dashboard Cite

Background: Osteosarcoma (OS) is a disease with high mortality in children and adolescents, and metastasis is one of its important clinical features. However, the molecular mechanism of OS occurrence is not completely clear. Thus, we screened potential biomarkers of OS and analyze their prognostic value. Methods:The Cancer Genome Atlas (TCGA) datasets were used to analyze the differential lncRNAs in patients with OS of different immune score and the lncRNAs expressed by immune cells. Cox regression was used to develop the prognosis prediction model and specify the prognosis outcomes. Risk-proportional regression model was constructed, and the samples were divided into high and low groups based on the risk scores for the survival analysis. The areas under the receiver operating characteristic (ROC) curve were calculated and the risk-score model was verified. Finally, using 4 gene sets (comprising chemokines, immune checkpoint blockades, immune activity-related genes, and immune cells), and 4 analysis tools (CIBERSORT, TIMER, XCELL and MCP) to evaluated tumor immune infiltration.Results: Twenty-nine long non-coding ribonucleic acids (lncRNAs) were obtained from the intersection of the screened lncRNAs. Caspase recruitment domain-containing protein 8-antisense RNA 1 (CARD8-AS1), lncRNA five prime to Xist (FTX), KAT8 regulatory NSL complex unit 1-antisense RNA 1 (KANSL1-AS1), Neuroplastin Intronic Transcript 1 (NPTN-IT1), oligodendrocyte maturation-associated long intervening non-coding RNA (OLMALINC) and RPARP Antisense RNA 1 (RPARP-AS1) were found to be correlated with survival. Univariate and multivariate regression analysis showed risk score [HR (hazard ratio) 3.5, P value 0.0043; HR 3.7, P value 0.0033] and metastasis (HR 4.7, P value 6.60E-05; HR 4.8, P value 8.36E-05) were the key factors of patients with OS. The areas under curves (AUCs) of the 1-, 3-, and 5-year ROC curves of the prognostic model were 0.715, 0.729, and 0.771. The low-risk patients tended to have a high abundance of immune cells.Conclusions: This study showed that a risk score based on 6 lncRNAs has potential value in the prognosis of OS, and patients with low-risk scores have high immune cell infiltration and good prognosis. This study may enrich understandings of underlying mechanisms related to the occurrence and development of OS.

show abstract

Section: Discussionmentioning

confidence: 99%

Bioinformatics analysis of lncRNAs in the occurrence and development of osteosarcoma

Liu¹,

Zong²,

Sun³

et al. 2022

Transl Pediatr

View full text Add to dashboard Cite

show abstract

“…C3AR1 mRNA expression was positively correlated to the infiltration levels of various immune cells such as macrophages [ 26 ]. EBI2 expression directs the date of Tfh cells by promoting their interaction with IL-2-quenching dendritic cells.…”

Section: Discussionmentioning

confidence: 99%

Single-Cell RNA-Sequencing Reveals the Active Involvement of Macrophage Polarizations in Pulmonary Hypertension

Mao

Yang

et al. 2022

Disease Markers

View full text Add to dashboard Cite

Background. Sustained hypoxia can trigger a progressive rise in pulmonary artery pressure and cause serious pulmonary diseases. Macrophages play important roles along the progression of pulmonary hypertension. However, the state of macrophage polarization during the early stage of pulmonary hypertension is unclear. Methods. Unlike traditional sequencing method, single-cell sequencing can accurately distinguish among cell types and better understand cell-to-cell relationships. In this study, we investigated the polarization of macrophages in pulmonary hypertension via single-cell RNA-sequencing in a mice hypoxia model, which was then validated in patients with pulmonary hypertension. Results. We identified that the intermittent exposure to hypoxic conditions could lead to the production of more M2-type macrophages than M1-type macrophages in a mouse model. Further validation analysis was performed by analyzing lung tissue of patients with pulmonary hypertension, revealing that the number of disease-associated M2 macrophages was substantially increased. Conclusions. In this study, the active anti-inflammatory response of macrophage involved in pulmonary hypertension has been identified, suggesting that intervention against the polarization of macrophages to the M2 type may be a potential way to reduce chronic pulmonary inflammation, pulmonary vascular remodeling, and artery pressure. Thus, investigation of macrophage polarization associated with hypoxia could help us better understand disease mechanism and craft effective prevention strategies and approaches.

show abstract

“…For instance, Zou et al. found that C3AR1 promoted the apoptosis of osteosarcoma cells and was closely related to the infiltration of several immune cells such as macrophages M1, macrophages M2, CD8 + T cells and so on [32] . In our research, the GSEA results revealed that the apoptosis-associated risk related to several immune pathways such as B cell receptor signaling pathway and intestinal immune network for IgA production.…”

Section: Discussionmentioning

confidence: 99%

Construction and validation of a novel apoptosis-associated prognostic signature related to osteosarcoma metastasis and immune infiltration

Jin

Shen

et al. 2022

Translational Oncology

View full text Add to dashboard Cite

C3AR1 mRNA as a Potential Therapeutic Target Associates With Clinical Outcomes and Tumor Microenvironment in Osteosarcoma

Cited by 13 publications

References 35 publications

Bioinformatics analysis of lncRNAs in the occurrence and development of osteosarcoma

Bioinformatics analysis of lncRNAs in the occurrence and development of osteosarcoma

Single-Cell RNA-Sequencing Reveals the Active Involvement of Macrophage Polarizations in Pulmonary Hypertension

Construction and validation of a novel apoptosis-associated prognostic signature related to osteosarcoma metastasis and immune infiltration

Contact Info

Product

Resources

About