Ca²⁺ dynamics in zebrafish hyper-dorsalization of congenital anomaly

Abstract

Intracellular calcium ion (Ca2+) signaling is deeply involved in development, as demonstrated by the use of various Ca2+ sensors. Ca2+ patterns in wildtype and mutant zebrafish have been reported to date, however, not many zebrafish embryos with congenital anomaly have been studied yet. In the present study, we monitored serial Ca2+ levels of zebrafish embryos which showed inborn hyper-dorsalization using Ca2+ sensor, yellow cameleon, YC2.12. Our results showed clearly elevated Ca2+ levels in the presumptive dorsal part during gastrula period and in the dorsal region during segmentation period. The results suggested that monitoring Ca2+ levels may help to anticipate the region which influences a fate determination prior to morphogenesis.