Ca²⁺dynamics in zebrafish morphogenesis

Abstract

Intracellular calcium ion (Ca2+) signaling is heavily involved in development, as illustrated by the use of a number of Ca2+ indicators. However, continuous Ca2+ patterns during morphogenesis have not yet been studied using fluorescence resonance energy transfer to track the Ca2+ sensor. In the present study, we monitored Ca2+ levels during zebrafish morphogenesis and differentiation with yellow cameleon, YC2.12. Our results show not only clear changes in Ca2+ levels but also continuous Ca2+ patterns at 24 hpf and later periods for the first time. Serial Ca2+dynamics during early pharyngula period (Prim-5-20; 24–33 hpf) was successfully observed with cameleon, which have not reported anywhere yet. In fact, high Ca2+ level occurred concurrently with hindbrain development in segmentation and pharyngula periods. Ca2+ patterns in the late gastrula through segmentation periods which were obtained with cameleon, were similar to those obtained previously with other Ca2+sensor. Our results suggested that the use of various Ca2+ sensors may lead to novel findings in studies of Ca2+ dynamics. We hope that these results will prove valuable for further research in Ca2+ signaling.

Modeling Human Muscular Dystrophies in Zebrafish: Mutant Lines, Transgenic Fluorescent Biosensors, and Phenotyping Assays

“…These indicators are based on modifications of calmodulin (CaM), in which the calciumbinding domain is fused to GFP. GCaMP indicators with different calcium sensitivities have been developed (GCaMP1, GcaMP3, GcaMP6, GcaMP6f, GcaMP6s, and GcaMP7) to check the cytoplasmic levels of calcium [147,149,150]. Modified GCaMP6f, which harbors a mitochondrial-specific leader peptide at the N-terminus, was developed to check calcium levels in mitochondria [151].…”

Ca2+ dynamics in zebrafish hyper-dorsalization of congenital anomaly

“…Intracellular calcium ions (Ca 2+ ) is very important in morphogenesis (Niki et al, 1996;Berridge, Lipp & Bootman, 2000;Slusarski & Pelegri, 2007). To date, consecutive Ca 2+ dynamics of zebrafish normal development was reported with fluorescent yellow cameleon YC2.12 (Tsuruwaka et al, 2007(Tsuruwaka et al, & 2016. We also reported that morphological changes brought about dramatic transition in Ca 2+ signaling (Tsuruwaka, Konishi & Shimada, 2015).…”

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“…Intracellular calcium ions (Ca 2+ ) is very important in morphogenesis (Niki et al, 1996; Berridge, (Tsuruwaka et al, 2007(Tsuruwaka et al, & 2016. We also reported that morphological changes brought about 35 dramatic transition in Ca 2+ signaling (Tsuruwaka, Konishi & Shimada, 2015 …”