Ca2+ extrusion across plasma membrane and Ca2+ uptake by intracellular stores

Contribution of Ca(2+)‐induced Ca2+ release to the [Ca2+]i transients in myocytes from guinea‐pig urinary bladder.

“…10). The equilibration period of 6 s can be attributed to the period necessary to increase the non-inactivating ICa as it was evaluated with a fast solution change (Hering, Beech, Bolton & Lim, 1988 (Imaizumi, Muraki, Takeda & Watanabe, 1989 Thapsigargin was applied as an inhibitor of the SR Ca2+-ATPase (Missiaen et al 1991 (Fig. 6) support this hypothesis.…”

“…1), as it was in ryanodine-and thapsigargin-treated cells (Figs 5, 6 and 7). In addition, there is evidence that only some of the SR Ca2+ stores are sensitive to thapsigargin (Missiaen et al 1991). Vice versa, ryanodine and caffeine interact only with the ryanodine, but not with the IP3, receptors, e.g.…”

“…When caffeine was applied again (Fig. 6, third The tumour promotor thapsigargin has been recently introduced as a blocker of the Ca2+,Mg2+-ATPase of endo-and sarcoplasmic reticulum (Kwan et al 1990;Thastrup et al 1990;Bian et al 1991; for references see review by Missiaen, Wuytack, Raeymaekers, De Smedt, Droogmans, Declerck & Casteels, 1991). The inhibition of the Ca2+ pump is expected to reduce the Ca2+ load of the store and, thus, (Fig.…”

Release of Ca2+ from the sarcoplasmic reticulum increases mitochondrial [Ca2+] in rat pulmonary artery smooth muscle cells

“…In smooth muscle cells, following removal of a contractile stimulus, [Ca¥]é is restored to resting values by ATPdependent Ca¥ pumps in the SR and plasma membrane (Missiaen et al 1991), and also by the plasma membrane Na¤-Ca¥ exchanger (McCarron et al 1994). The role of the mitochondria in this process is far less certain, although recent studies utilizing pharmacological approaches have clearly implicated mitochondrial involvement Greenwood et al 1997).…”

Ca2+ clearance mechanisms in isolated rat adrenal chromaffin cells.

“…12C). In interpreting this experiment one should note that ryanodine-and caffeine-sensitive Ca2+ channels are also inhibited by Ruthenium Red and that plasma membrane Ca2+-ATPases may be as well (Missiaen et al 1993). Fig.…”