Ca2+ Extrusion via Na+-Ca2+ Exchangers in Rat Odontoblasts

Tsumura, Maki; Okumura, Reijiro; Tatsuyama, Shoko; Ichikawa, Hideki; Muramatsu, Takashi; Matsuda, Takehisa; Baba, Akemichi; Suzuki, Keiko; Kajiya, Hiroshi; Sahara, Yoshinori; Tokuda, Masanori; Momose, Yasunori; Tazaki, Masakazu; Shimono, Masaki; Shibukawa, Yoshiyuki

doi:10.1016/j.joen.2010.01.006

Cited by 33 publications

(33 citation statements)

References 25 publications

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“…Na + /Ca 2+ exchangers contribute to calcium transport in many tissues, including the tooth organ as discussed recently for NCX1 and NCX3 [Okumura et al, 2010; Tsumura et al, 2010]. In this study, we demonstrate that NCKX4 is expressed in late secretory-stage and maturation-stage ameloblasts, with significantly increased mRNA and protein expression during the late stages of amelogenesis.…”

Section: Discussionsupporting

confidence: 75%

“…Recently the expression of NCX1 and NCX3 was demonstrated in ameloblasts [Okumura et al, 2010] and odontoblasts [Tsumura et al, 2010]. In this study, we present evidence for mRNA expression for all the 6 members of the Slc24a gene family in the developing mouse molar organ, with the highest expression by Slc24a4/NCKX4.…”

Section: Introductionsupporting

confidence: 59%

“…The importance of this ionic movement is, nevertheless, well appreciated [Smith, 1998; Hubbard, 2000]. During dentin formation, Ca 2+ is transported from the pulpal vascular network through the odontoblast layer, by what is believed to be a transcellular pathway, to be incorporated in the mineral phase at the interface between the nonmineralized pre-dentin and the mineralized dentin [Linde, 1995; Tsumura et al, 2010]. Mature enamel is composed of densely packed Hap crystallites arranged into enamel prisms [Paine et al, 2001].…”

Section: Introductionmentioning

confidence: 99%

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Expression of the Sodium/Calcium/Potassium Exchanger, NCKX4, in Ameloblasts

Lacruz²,

Smith

et al. 2012

Cells Tissues Organs

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Transcellular calcium transport is an essential activity in mineralized tissue formation, including dental hard tissues. In many organ systems, this activity is regulated by membrane-bound sodium/calcium (Na⁺/Ca²⁺) exchangers, which include the NCX and NCKX [sodium/calcium-potassium (Na⁺/Ca²⁺-K⁺) exchanger] proteins. During enamel maturation, when crystals expand in thickness, Ca²⁺ requirements vastly increase but exactly how Ca²⁺ traffics through ameloblasts remains uncertain. Previous studies have shown that several NCX proteins are expressed in ameloblasts, although no significant shifts in expression were observed during maturation which pointed to the possible identification of other Ca²⁺ membrane transporters. NCKX proteins are encoded by members of the solute carrier gene family, Slc24a, which include 6 different proteins (NCKX1–6). NCKX are bidirectional electrogenic transporters regulating Ca²⁺ transport in and out of cells dependent on the transmembrane ion gradient. In this study we show that all NCKX mRNAs are expressed in dental tissues. Real-time PCR indicates that of all the members of the NCKX group, NCKX4 is the most highly expressed gene transcript during the late stages of amelogenesis. In situ hybridization and immunolocalization analyses clearly establish that in the enamel organ, NCKX4 is expressed primarily by ameloblasts during the maturation stage. Further, during the mid-late maturation stages of amelogenesis, the expression of NCKX4 in ameloblasts is most prominent at the apical poles and at the lateral membranes proximal to the apical ends. These data suggest that NCKX4 might be an important regulator of Ca²⁺ transport during amelogenesis.

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Section: Discussionsupporting

confidence: 75%

Section: Introductionsupporting

confidence: 59%

Section: Introductionmentioning

confidence: 99%

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Expression of the Sodium/Calcium/Potassium Exchanger, NCKX4, in Ameloblasts

Lacruz²,

Smith

et al. 2012

Cells Tissues Organs

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“…Odontoblasts localized on the rim of the primary cultured dental pulp slices were loaded with fura-2, and [Ca 2+ ] i was measured [23]–[26]. Menthol (100 µM; Figure 2A) and icilin (1 µM; Figure 2B), TRPM8 and TRPA1 channel agonists, increased [Ca 2+ ] i to peak values of 1.03±0.01 F/F 0 units ( N = 4) and 1.07±0.003 F/F 0 units ( N = 3), respectively (Figure 2E).…”

Section: Resultsmentioning

confidence: 99%

Functional Expression of TRPM8 and TRPA1 Channels in Rat Odontoblasts

et al. 2013

Self Cite

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Odontoblasts produce dentin during development, throughout life, and in response to pathological conditions by sensing stimulation of exposed dentin. The functional properties and localization patterns of transient receptor potential (TRP) melastatin subfamily member 8 (TRPM8) and ankyrin subfamily member 1 (TRPA1) channels in odontoblasts remain to be clarified. We investigated the localization and the pharmacological, biophysical, and mechano-sensitive properties of TRPM8 and TRPA1 channels in rat odontoblasts. Menthol and icilin increased the intracellular free Ca2+ concentration ([Ca2+]i). Icilin-, WS3-, or WS12-induced [Ca2+]i increases were inhibited by capsazepine or 5-benzyloxytriptamine. The increase in [Ca2+]i elicited by allyl isothiocyanate (AITC) was inhibited by HC030031. WS12 and AITC exerted a desensitizing effect on [Ca2+]i increase. Low-temperature stimuli elicited [Ca2+]i increases that are sensitive to both 5-benzyloxytriptamine and HC030031. Hypotonic stimulation-induced membrane stretch increased [Ca2+]i; HC030031 but not 5-benzyloxytriptamine inhibited the effect. The results suggest that TRPM8 channels in rat odontoblasts play a role in detecting low-temperature stimulation of the dentin surface and that TRPA1 channels are involved in sensing membrane stretching and low-temperature stimulation. The results also indicate that odontoblasts act as mechanical and thermal receptor cells, detecting the stimulation of exposed dentin to drive multiple cellular functions, such as sensory transduction.

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“…Overall, the Ca 2+ extrusion mechanism that operates by way of NCX in the distal membrane of odontoblasts plays a specific role in driving cellular activities such as dentinogenesis via a directional Ca 2+ transport pathway from the circulation to the dentin‐mineralizing front (Tsumura et al, ). However, current research as for the expression and function of NCX in tooth‐derived cells and intracellular organelles is rarely reported and lacks data on human DPCs and odontoblasts.…”

Section: Ca2+ Sensors–transporters and Intracellular Ca2+ Signalsmentioning

confidence: 99%

The emerging role of extracellular Ca²⁺ in osteo/odontogenic differentiation and the involvement of intracellular Ca²⁺ signaling: From osteoblastic cells to dental pulp cells and odontoblasts

2018

Journal Cellular Physiology

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Calcium ions (Ca ) is the main element of dental pulp capping materials. Ca signaling plays a crucial role in a myriad of cell activities. An overwhelming array of studies have already reported the experimental and clinical benefits of Ca -enriched materials in the treatment of teeth with accidental vital pulp exposure and incomplete root formation. Thus, Ca signaling has always been an excellent target for the design of various novel biomaterials for use in revitalizing or regenerative endodontic procedures. However, the molecular mechanisms that enable dental pulp cells (DPCs) to detect and respond to extracellular Ca have not been characterized in detail before. In this review, we mainly outline the pathways by which the cell detects and responds to extracellular Ca , as well as the relevant regulatory paths in DPCs and odontoblasts, and discuss the potential role of Ca as a therapeutic tool. Moreover, because DPCs share many of the same functional properties that are found in osteoblasts, some comparisons with bone cells were additionally incorporated into this text.

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Ca2+ Extrusion via Na+-Ca2+ Exchangers in Rat Odontoblasts

Cited by 33 publications

References 25 publications

Expression of the Sodium/Calcium/Potassium Exchanger, NCKX4, in Ameloblasts

Expression of the Sodium/Calcium/Potassium Exchanger, NCKX4, in Ameloblasts

Functional Expression of TRPM8 and TRPA1 Channels in Rat Odontoblasts

The emerging role of extracellular Ca²⁺ in osteo/odontogenic differentiation and the involvement of intracellular Ca²⁺ signaling: From osteoblastic cells to dental pulp cells and odontoblasts

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Ca2+ Extrusion via Na+-Ca2+ Exchangers in Rat Odontoblasts

Cited by 33 publications

References 25 publications

Expression of the Sodium/Calcium/Potassium Exchanger, NCKX4, in Ameloblasts

Expression of the Sodium/Calcium/Potassium Exchanger, NCKX4, in Ameloblasts

Functional Expression of TRPM8 and TRPA1 Channels in Rat Odontoblasts

The emerging role of extracellular Ca2+ in osteo/odontogenic differentiation and the involvement of intracellular Ca2+ signaling: From osteoblastic cells to dental pulp cells and odontoblasts

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Product

Resources

About

The emerging role of extracellular Ca²⁺ in osteo/odontogenic differentiation and the involvement of intracellular Ca²⁺ signaling: From osteoblastic cells to dental pulp cells and odontoblasts