Cadherin-1, -2 and -4 expression in the cranial ganglia and lateral line system of developing zebrafish

Cadherin‐4 plays a role in the development of zebrafish cranial ganglia and lateral line system

“…Moreover, even in those morphants in which the nP reached the tail region, the nerve was thinner than in the control embryos. The nP defect in the morphants correlates well with Cdh4/cdh4 expression in the system (Liu et al, 2003), suggesting that Cdh4 is involved in the extension of the nerve. Moreover, the nP defects in the cdh4 morphants are different from those of embryos with disrupted cadherin-2 function, in which the nP had greatly altered trajectories (curved or turned around), instead of reduced length (Kerstetter et al, 2004).…”

“…This was not surprising since cdh4 expression is detected in the gVIII of slightly younger embryos (26 hpf), and not observed in other ganglia until 32 hpf (Liu et al, 2003). In contrast, disruption of cadherin2 expression (expressed throughout the development of most of the cranial and lateral line ganglia; Liu et al, 2003) in developing zebrafish resulted in detectable abnormalities in these ganglia as early as 25 hpf (Kerstetter et al, 2004). However, by 50 -55 hpf, development of the ganglia was disrupted in the vast majority of cdh4 morphants (Table 1).…”

“…Cdh4 expression and/or function in the vertebrate nervous system has been studied in a variety of vertebrate species including zebrafish, Xenopus, chicken, and mouse (Inuzuka et al, 1991;Redies et al, 1992;Ganzler and Redies, 1995;Matsunami and Takeichi, 1995;Tashiro et al, 1995;Liu et al, 1999;Wohrn et al, 1998;Gerhardt et al, 2000;Honjo et al, 2000;Treubert-Zimmermann et al, 2002;Andrews and Mastick, 2003;Babb et al, 2005). We previously showed that Cdh4/cdh4 is expressed in the majority of the cranial and lateral line ganglia and their nerves in the developing zebrafish (Liu et al, 2003). We hypothesize that interfering with Cdh4 function disrupts formation of these structures.…”

“…They arrive in the tail by 46 -48 hpf (Metcalfe, 1985(Metcalfe, , 1989. The expression pattern of Cdh4/cdh4 in the gP and nP of developing zebrafish (Liu et al, 2003) suggests that Cdh4 participates in the formation of the ganglion (see above) and extension of the nerve. Anti-acetylated tubulin immunostaining showed that the nP reached the tail region of all control and 5-mis MO-injected embryos at 50 -55 hpf ( Fig.…”

“…Liu and A.L. Wilson, unpublished observation), while the vagal ganglion (gX) was Cdh4 immunoreactive at 45 hpf (Liu et al, 2003). Using multiple markers, we analyzed organization of these structures in cdh4 morphants, and compared it with that of control embryos and those injected with the 5-mis MO.…”