Cadmium biosorption by yeast, Candida tropicalis CBL-1, isolated from industrial wastewater

Rehman, Abdul; Anjum, Muhammad Sohail; Hasnain, Shahida

doi:10.2323/jgam.56.359

Cited by 27 publications

(25 citation statements)

References 45 publications

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“…Red cell GSH and GSSG levels were measured using Ellman’s reagent by the reduction of 5, 5ˊ- dithiobis-2-nitrobenzoic acid (DNTB) as described previously [ 20 ]. The reaction buffer consisted of 0.1 M PO 4 buffer (pH 7), 0.5 mM EDTA and low concentration of DNTB (3 mM) in absolute ethanol.…”

Section: Methodsmentioning

confidence: 99%

The SNP rs10911021 is associated with oxidative stress in coronary heart disease patients from Pakistan

2018

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Backgroundrs10911021 (a single nucleotide polymorphism present upstream of the GLUL gene) affects glutamic acid metabolism, and was shown to be associated with coronary heart disease (CHD) in patients with T2DM but a definite mechanism is unknown. It may affect glutathione cycle, an important effector in the antioxidant defense mechanism, in the cells. We checked the association of this SNP with CHD and oxidative stress biomarkers, malondialdeheyde (MDA), GSH and GSSG in Pakistani patients.MethodsA total of 650 subjects (425 CHD cases and 225 controls) were genotyped by TaqMan allelic discrimination technique. The levels of MDA, GSH and GSSG were measured by standard protocols.ResultsThe risk allele frequency was higher in cases than controls, but the difference was insignificant (p = 0.55). The SNP was not associated with CHD (p = 0.053) but when the analysis was limited to CHD patients having DM, a significant association (p = 0.03) was observed. The blood levels of MDA and GSSG were higher while that of GSH was significantly lower in the cases than the controls (p < 0.05). Each risk allele increased MDA and GSSG by 0.29 (0.036) mmol/l and 0.4 (0.04) mmol/l, respectively, while decreased GSH by −0.36 (0.03) mmol/l. The SNP was not associated with any of the tested blood lipids.ConclusionThe SNP rs10911021 was associated with CHD only in patients having diabetes, but the SNP was associated with total oxidative stress biomarkers MDA and GSH and GSSG levels. As the SNP rs10911021 showed significant association with oxidative stress parameters and these parameters should an increased oxidative stress in the CHD subjects, it can be concluded that the SNP may have contributed to increase the risk of heart diseases in the diabetic subjects by increasing the oxidative stress.

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Section: Methodsmentioning

confidence: 99%

The SNP rs10911021 is associated with oxidative stress in coronary heart disease patients from Pakistan

2018

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“…For the estimation of reduced (GSH), oxidized (GSSG) and total glutathione contents; methods described by Rehman et al . and Israr et al . were followed with modifications.…”

Section: Methodsmentioning

confidence: 90%

“…For the estimation of reduced (GSH), oxidized (GSSG) and total glutathione contents; methods described by Rehman et al [18] and Israr et al [19] were followed with modifications. The bacterial cultures were grown in MOPS Milieu medium (pH 7.0) supplemented with 4 g L À1 sodium succinate and 1 mM phosphate.…”

Section: Estimation Of Glutathione and Other Non-protein Thiolsmentioning

confidence: 99%

Antioxidative enzyme profiling and biosorption ability of Cupriavidus metallidurans CH34 and Pseudomonas putida mt2 under cadmium stress

Shamim

Rehman

2013

J. Basic Microbiol.

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Cupriavidus metallidurans CH34 and Pseudomonas putida mt2 were used as cadmium (Cd)-resistant and -sensitive bacteria, respectively, to study their biosorption ability and their antioxidative enzymes. The minimal inhibitory concentration of C. metallidurans CH34 for Cd was found to be 30 mM, and for P. putida mt2 it was 1.25 mM. The tube dilution method revealed the heavy-metal resistance pattern of C. metallidurans CH34 as Ni(2+) (10 mM)>Zn(2+) (4 mM)>Cu(2+) (2 mM)>Hg(2+) (1 mM)>Cr(2+) (1 mM)>Pb(2+) (0 mM), whereas P. putida mt2 was only resistant to Zn(2+) (1 mM). Under Cd stress, the induction of GSH was higher in C. metallidurans CH34 (0.359 ± 0.010 mM g(-1) FW) than in P. putida mt2 (0.286 ± 0.005 mM g(-1) FW). Glutathione reductase was more highly expressed in the mt2 strain, in contrast to non-protein thiols and peroxidase. Unlike dead bacterial cells, live cells of both bacteria showed significant Cd biosorption, i.e. more than 80% at 48 h. C. metallidurans CH34 used only catalase, whereas P. putida mt2 used superoxide dismutase and ascorbate peroxidase to combat Cd stress. This study investigated the Cd biosorption ability and enzymes involved in the Cd detoxification mechanisms of C. metallidurans CH34 and P. putida mt2.

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“…Previous studies suggested that Candida spp. were the most frequently cultured fungi recovered from anoxic-activated sludge samples [36] and industrial wastewater [37]. Members of the genus Candida have the ability to adsorb and/or accumulate metals from their surroundings, also in addition to their ability to produce extracellular polymeric substances for biofilm formation, flocculation, and adhesion processes [20].…”

Section: Glomeromycotamentioning

confidence: 99%

Industrial Effluents Harbor a Unique Diversity of Fungal Community Structures as Revealed by High-throughput Sequencing Analysis

Selvarajan¹,

Sibanda²,

Sekar³

et al. 2019

Pol. J. Environ. Stud.

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The actual extent of fungal diversity in different environmental media is still a subject of ongoing research. Little is currently known about the diversity of fungal populations in industrial streams. This study characterized the fungal diversity of different industrial effluents using a high-throughput sequencing approach. A total of 234617 quality filtered reads were obtained from the collected wastewater samples. Phylogenetic taxonomy revealed that resident fungal communities were classified as 6 phyla, 31 classes, 79 orders, 144 families, and 192 genera. Ascomycota and Basidiomycota were the most dominant phyla whose relative abundance ranged from 23.29% to 38.31%, and 17.34% to 33.51%, respectively. Recovered operational taxonomic units (OTUs) ranged from 292 (Dixon) to 427 (Capegate). The existence of some fungal genera identified in the industrial wastewaters correlated to physicochemical variables and had the potential to play important roles in organic decomposition, pollutant degradation, and xenobiotic transformation. Meanwhile, the occurrence of unclassified fungal sequences (22.5% to 33.09%) suggests that these effluents are a potential reservoir of as-yet uncharacterized fungal species.

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Cadmium biosorption by yeast, Candida tropicalis CBL-1, isolated from industrial wastewater

Cited by 27 publications

References 45 publications

The SNP rs10911021 is associated with oxidative stress in coronary heart disease patients from Pakistan

The SNP rs10911021 is associated with oxidative stress in coronary heart disease patients from Pakistan

Antioxidative enzyme profiling and biosorption ability of Cupriavidus metallidurans CH34 and Pseudomonas putida mt2 under cadmium stress

Industrial Effluents Harbor a Unique Diversity of Fungal Community Structures as Revealed by High-throughput Sequencing Analysis

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