Cadmium localization and quantification in the plant Arabidopsis thaliana using micro-PIXE

Ager, F.J.; Ynsa, M.D.; Domı́nguez-Solı́s, José R.; Gotor, Cecilia; Respaldiza, M.Á.; Romero, Luís C.

doi:10.1016/s0168-583x(01)01130-2

Cited by 58 publications

(34 citation statements)

References 8 publications

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“…Subcellular localization is in accordance with our results with striking cellular distribution in a narrow ring or thick band at the trichome base (Küpper et al, 2000;Zhao et al, 2000;Sarret et al, 2002;Fukuda et al, 2008), corresponding to the secondary cell wall above the OR. There is also evidence of cadmium hyperaccumulation in trichomes of Arabidopsis (Ager et al, 2002). Jakoby et al (2008) have shown that zinc transporters and copper chaperones are on the list of the 5% most up-regulated genes in the mature trichome.…”

Section: Role Of the Or And The Autofluorescent Secondary Cell Wall Lmentioning

confidence: 99%

Cell Wall Maturation of Arabidopsis Trichomes Is Dependent on Exocyst Subunit EXO70H4 and Involves Callose Deposition

et al. 2015

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Arabidopsis (Arabidopsis thaliana) leaf trichomes are single-cell structures with a well-studied development, but little is understood about their function. Developmental studies focused mainly on the early shaping stages, and little attention has been paid to the maturation stage. We focused on the EXO70H4 exocyst subunit, one of the most up-regulated genes in the mature trichome. We uncovered EXO70H4-dependent development of the secondary cell wall layer, highly autofluorescent and callose rich, deposited only in the upper part of the trichome. The boundary is formed between the apical and the basal parts of mature trichome by a callose ring that is also deposited in an EXO70H4-dependent manner. We call this structure the Ortmannian ring (OR). Both the secondary cell wall layer and the OR are absent in the exo70H4 mutants. Ecophysiological aspects of the trichome cell wall thickening include interference with antiherbivore defense and heavy metal accumulation. Ultraviolet B light induces EXO70H4 transcription in a CONSTITUTIVE PHOTOMORPHOGENIC1-dependent way, resulting in stimulation of trichome cell wall thickening and the OR biogenesis. EXO70H4-dependent trichome cell wall hardening is a unique phenomenon, which may be conserved among a variety of the land plants. Our analyses support a concept that Arabidopsis trichome is an excellent model to study molecular mechanisms of secondary cell wall deposition.

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Section: Role Of the Or And The Autofluorescent Secondary Cell Wall Lmentioning

confidence: 99%

Cell Wall Maturation of Arabidopsis Trichomes Is Dependent on Exocyst Subunit EXO70H4 and Involves Callose Deposition

et al. 2015

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“…The Cd in Z. mays was bound to the cell walls, which could explain increased Cd-tolerance. In plants such as Arabidopsis thaliana (Ager et al, 2002;2003) and the Cd-hyperaccumulator Biscutella laevigata (Pielichowska and Wierzbicka, 2004), some of the Cd that is translocated is ultimately sequestered and rendered non-toxic in trichomes.…”

Section: Introductionmentioning

confidence: 99%

“…Images of the distribution of Cd in plants can also be obtained by using scanning electron microscopy (SEM) or transmission electron microscopy (TEM) along with energy dispersive X-ray spectroscopy (EDS, Solís-Domínguez et al, 2007;Hu et al, 2009) or energy dispersive X-ray (EDX) microanalysis (Rauser and Ackerley, 1987;Wójcik et al, 2005;Van Belleghem et al, 2007;Vazquez et al, 2007;Cocozza et al, 2008). Other imaging techniques include secondary ion mass spectrometry (SIMS, Migeon et al, 2011), micro-particle-induced X-ray emission (µ-PIXE, Ager et al, 2002;Vogel-Mikuš et al, 2008), and micro-autoradiography . While the techniques listed above are able to detect Cd-specific signals in particular regions within a tissue, the low concentrations of Cd in the cells and the proximity of the emission spectra from Ca or K in the sample can make analysis difficult.…”

Section: Introductionmentioning

confidence: 99%

Localization and chemical speciation of cadmium in the roots of barley and lettuce

Akhter

Omelon

Gordon

et al. 2014

Environmental and Experimental Botany

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Plants have the potential to accumulate toxic amounts of cadmium (Cd), and understanding how and where Cd is stored in plants is important for ensuring food safety.Previous experiments have determined that a greater amount of Cd is translocated into the leaves of lettuce (Lactuca sativa) as compared to barley leaves (Hordeum vulgare).Preferential retention of Cd in root of barley would explain this difference. Hence, the purpose of this study was to determine the localization and coordination environment of Cd (i.e., the ligands to which Cd was bound) in the different root tissues of lettuce and barley using histochemical staining, electron microscopy and micro X-ray spectroscopy.Retention of Cd in barley roots could be explained by accumulation of Cd at the endodermis, comparatively higher amounts of Cd sequestered in the symplast of cortical cells and binding to xylem cell walls. Increased translocation of Cd to lettuce shoots seemed to be due to a less effective barrier at the endodermis and less sequestration of Cd in the cortex. Regardless of the tissue type, most of the Cd 2+ was bound to S ligands in the roots of barley, possibly reflecting accumulation of Cd-phytochelatin and Cd-S molecules in the vacuoles. In lettuce roots, Cd was more evenly distributed among ligands containing S, O and NO 3 groups, which is indicative of proportionately more Cd binding to the cell walls, relative to barley. These results will be useful in uncovering the mechanisms of differential Cd-tolerance and sequestration in lettuce and barley.

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“…Keywords: EDXMA, heavy metals, poplar, pollution, SEM tracellular Cd sequestration in poplars. There are different methods for determining ele ments distribution in plant tissues such as histochemical (Vázquez et al 1992b, Seregin & Ivanov 1997, cell fractionation (LozanoRodríguez et al 1997), x-ray microanalysis (Khan et al 1984, Vázquez et al 1992a, Vázquez et al 1992b, particle-induced x-ray emission (micro-PIXE - Ager et al 2002) or nuclear micro-probe technique (NMP -Ager et al 2003). Although not always useful at very low metal concentrations, x-ray mi croanalysis provides specific signals com pared to other methods (Wójcik et al 2005(Wójcik et al et al 2005.…”

Section: Introductionmentioning

confidence: 99%

Distribution and concentration of cadmium in root tissue of Populus alba determined by scanning electron microscopy and energy-dispersive x-ray microanalysis

Cocozza¹,

Minnocci²,

Tognetti³

et al. 2008

iForest

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Cadmium localization and quantification in the plant Arabidopsis thaliana using micro-PIXE

Cited by 58 publications

References 8 publications

Cell Wall Maturation of Arabidopsis Trichomes Is Dependent on Exocyst Subunit EXO70H4 and Involves Callose Deposition

Cell Wall Maturation of Arabidopsis Trichomes Is Dependent on Exocyst Subunit EXO70H4 and Involves Callose Deposition

Localization and chemical speciation of cadmium in the roots of barley and lettuce

Distribution and concentration of cadmium in root tissue of Populus alba determined by scanning electron microscopy and energy-dispersive x-ray microanalysis

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