Cafestol increases fat oxidation and energy expenditure in Caenorhabditis elegans via DAF-12-dependent pathway

Farias-Pereira, Renalison; Kim, Edward; Park, Yeonhwa

doi:10.1016/j.foodchem.2019.125537

Cited by 23 publications

(31 citation statements)

References 39 publications

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“…Signals from reproductive tissues regulate the lifespan of many organisms [ 32 ]. Germline signals regulate stress resistance, lipid metabolism, and lifespan through nuclear transcription factors DAF-16, DAF-12, and NHR-80 [ 33 – 35 ]. Here, to test if SDG could affect the transcriptional activity of these transcription factors, we analyzed the mRNA level of genes downstream of DAF-16, DAF-12, and NHR-80 in C. elegans , including sod-3 , fard-1 , and fat-6 [ 36 ], respectively.…”

Section: Resultsmentioning

confidence: 99%

Secoisolariciresinol Diglucoside Delays the Progression of Aging-Related Diseases and Extends the Lifespan ofCaenorhabditis elegansvia DAF-16 and HSF-1

Tan

Zhou

et al. 2020

Oxidative Medicine and Cellular Longevity

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Secoisolariciresinol diglucoside (SDG) is a phytoestrogen and rich in food flaxseed, sunflower seeds, and sesame seeds. Among the beneficial pharmacological activities of SDG on health, many are age related, such as anticancer, antidiabetes, antioxidant, and neuroprotective effects. Thus, we investigated if SDG had an effect on antiaging in Caenorhabditis elegans (C. elegans). Our results showed that SDG could extend the lifespan of C. elegans by up to 22.0%, delay age-related decline of body movement, reduce the lethality of heat and oxidative stress, alleviate dopamine neurodegeneration induced by 6-hydroxydopamine (6-OHDA), and decrease the toxicity of Aβ protein in C. elegans. SDG could increase the expression of the downstream genes of DAF-16, DAF-12, NHR-80, and HSF-1 at mRNA level. SDG could not extend the lifespan of mutants from genes daf-16, hsf-1, nhr-80, daf-12, glp-1, eat-2, and aak-2. The above results suggested that SDG might enhance the stress resistance, delay the progression of aging-related diseases, and extend the lifespan of C. elegans via DAF-16 and HSF-1.

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Section: Resultsmentioning

confidence: 99%

Secoisolariciresinol Diglucoside Delays the Progression of Aging-Related Diseases and Extends the Lifespan ofCaenorhabditis elegansvia DAF-16 and HSF-1

Tan

Zhou

et al. 2020

Oxidative Medicine and Cellular Longevity

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“…In order to quantify worms' body size by measuring width (μm) and length (μm), videos of nematodes were recorded and analyzed by WormLab tracking system and software (model MSCOP-002, software v. 3.1.0 64-bit, MBF Bioscience, MicroBrightField Inc., Willinston, VT, USA) ( Shen et al, 2018c ). Energy expenditure was estimated by the moving speed [μm/s; (forward distance + reverse distance)/time], since the locomotor behavior of C. elegans is related to energy expenditure ( Farias-Pereira et al., 2020 , Laranjeiro et al., 2017 , Shen et al., 2018b ).…”

Section: Methodsmentioning

confidence: 99%

“…Human lipid metabolism pathways are conserved in C. elegans , which help to identify the mechanisms of action of food components prior to vertebrate animals and/or humans ( Shen et al., 2018a , Shen et al., 2018b ). Previously, studies have examined the anti-obesity effects of plant extracts and food bioactive components in C. elegans , showing the relevance of this animal model for the mechanistic studies of prospective bioactives ( Bhattacharya et al., 2013 , Farias-Pereira et al., 2018 , Farias-Pereira et al., 2020 , Lin et al., 2019 , Liu et al., 2018 , Machado et al., 2018 , Shen et al., 2017 , Shen et al., 2018a , Shen et al., 2018b , Shen et al., 2018c , Sun et al., 2016 , Yue et al., 2019 ). Thus, the goal of this study was to determine the effects of isorhamnetin on lipid metabolism by using C. elegans as an animal model.…”

Section: Introductionmentioning

confidence: 99%

Fat-lowering effects of isorhamnetin are via NHR-49-dependent pathway in Caenorhabditis elegans

Farias-Pereira

Savarese

Yue

et al. 2020

Current Research in Food Science

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Isorhamnetin (3- O -methylquercetin), a flavonol found in dill weed, sea buckthorn berries, kale and onions, has been suggested to have anti-obesity effects, but there is limited evidence of its mechanisms of action on lipid metabolism. The goal of this study was to investigate the effects of isorhamnetin on lipid metabolism using Caenorhabditis elegans as an animal model. Isorhamnetin reduced fat accumulation without affecting food intake or energy expenditure in C. elegans . The isorhamnetin's fat-lowering effects were dependent on nhr-49 , a homolog of the human peroxisome proliferator-activated receptor alpha (PPARα). Isorhamnetin upregulated an enoyl-CoA hydratase ( ech-1.1 , involved in fatty acid β-oxidation) and adipose triglyceride lipase ( atgl-1 , involved in lipolysis) via NHR-49-dependent pathway at transcriptional levels. Isorhamnetin also upregulated the C. elegans AMP-activated protein kinase (AMPK) subunits homologs ( aak-1 and aak-2 ), involved in energy homeostasis. These results suggest that isorhamnetin reduces body fat by increasing fat oxidation in part via NHR-49/PPARα-dependent pathway.

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“…28 In C. elegans, the deletion of tub-1 (the homolog of the mammalian Tubby) causes an increased fat accumulation phenotype, which may be due to changes on lipid metabolism. 19,20,28 Our results showed that butein did not regulate tub-1 expression in wild-type C. elegans (Figure 2a). Moreover, butein reduced triglyceride content (p = .0095) compared to the control in tub-1 mutant (Figure 3c), suggesting that tub-1 is not a genetic requirement for the reduced triglyceride content by butein in C. elegans.…”

Section: The Effects Of Butein On Lipid Metabolism-related Transcrimentioning

confidence: 74%

“…12,18 2.4 | Pharynx pumping rate and moving speed Pharynx pumping rate in 30 s was measured to estimate food intake by using a stereomicroscope as previously described. 19 Videos (1 min) were recorded by using WormLab tracking system (model MSCOP-002, MBF Bioscience, MicroBrightField Inc., Willinston, VT), then videos were analyzed by WormLab software (3.1.0 64-bit, MBF Bioscience, Williston, VT) to get the nematode's moving speed, an indirect measurement of energy expenditure. 19…”

Section: Triglyceride Quantificationmentioning

confidence: 99%

Butein inhibits lipogenesis in Caenorhabditis elegans

et al. 2020

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Butein, a flavonoid found in annatto seeds and lacquer trees, may be used for many health benefits, including the prevention of obesity. However, its antiobesity effects are not completely understood; in particular, the effects of butein on the regulation of lipid metabolism have not been explained. Thus, the goal of the current study was to determine the effects of butein on lipid metabolism in Caenorhabditis elegans, which is a multi-organ nematode used as an animal model in obesity research. Butein at 70 μM reduced triglyceride content by 27% compared to the control without altering food intake and energy expenditure. The reduced triglyceride content by butein was associated with the downregulation of sbp-1, fasn-1, and fat-7, the lipogenesis-related homologs of sterol regulatory element-binding proteins, fatty acid synthase and stearoyl-CoA desaturase, respectively. Furthermore, fat-7 and skn-1, a homolog of nuclear respiratory factors, were identified as genetic requirements for butein's effects on triglyceride content in C. elegans. The effects of butein on sbp-1 and fasn-1 were dependent on skn-1, but the downregulation of fat-7 was independent of skn-1. These results suggest that the inhibitory effects of butein on lipogenesis are via SKN-1-and FAT-7-dependent pathways in C. elegans.

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Cafestol increases fat oxidation and energy expenditure in Caenorhabditis elegans via DAF-12-dependent pathway

Cited by 23 publications

References 39 publications

Secoisolariciresinol Diglucoside Delays the Progression of Aging-Related Diseases and Extends the Lifespan ofCaenorhabditis elegansvia DAF-16 and HSF-1

Secoisolariciresinol Diglucoside Delays the Progression of Aging-Related Diseases and Extends the Lifespan ofCaenorhabditis elegansvia DAF-16 and HSF-1

Fat-lowering effects of isorhamnetin are via NHR-49-dependent pathway in Caenorhabditis elegans

Butein inhibits lipogenesis in Caenorhabditis elegans

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