Caffeic acid phenethyl ester induces growth arrest and apoptosis of colon cancer cells via the β-catenin/T-cell factor signaling

Xiang, Dingcheng; Wang, Dong; He, Yonghong; Xie, Jing; Zhong, Zhaoyang; Li, Zengpeng; Xie, Jiang

doi:10.1097/01.cad.0000224441.01082.bb

Cited by 109 publications

(81 citation statements)

References 42 publications

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“…Western blot was carried out as described previously. 26 Briefly, equal protein aliquots (40 mg for whole cell, 10 mg for nuclear fractions) in each sample were resolved in 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis and the proteins transferred onto PVDF membranes. After blocking with 5% non-fat dried milk, the membranes were incubated with a 1:2000 dilution of primary mouse monoclonal antibodies against human APE1, b-actin and retinoblastoma (Rb).…”

Section: Western Blot Analysismentioning

confidence: 99%

Chimeric adenoviral vector Ad5/F35-mediated APE1 siRNA enhances sensitivity of human colorectal cancer cells to radiotherapy in vitro and in vivo

Wang

et al. 2008

Cancer Gene Ther

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Apurinic/apyrimidinic endonuclease (APE1), a bifunctional AP endonuclease/redox factor, is important in DNA repair and redox signaling, may be associated with radioresistance. Here we investigate whether targeted inhibition of APE1 can sensitize tumor cells to irradiation in vitro and in vivo. We first constructed chimeric adenoviral vector Ad5/F35 carrying human APE1 siRNA (Ad5/ F35-APE1 siRNA). The infectivity of chimeric Ad5/F35 to LOVO colon cancer cells was greater than that of Ad5. APE1 was strongly expressed and nuclear factor kB (NF-kB), a downstream molecule of APE1, known as a radioresistance factor, was constitutively active in LOVO cells. Infection of LOVO cells with Ad5/F35-APE1 siRNA resulted in a dose-dependent decrease of APE1 protein and AP endonuclease activity in vitro. Ad5/F35-APE1 siRNA significantly enhanced sensitivity of LOVO cells to irradiation in clonogenic survival assays, associated with increased cell apoptosis. The APE1 expression in LOVO cells was induced by irradiation in a dose-dependent manner, accompanied with the enhancement of DNA-binding activity of NF-kB and Ad5/F35-APE1 siRNA effectively inhibited constitutive and irradiation-induced APE1 expression and NF-kB activation. In a subcutaneous nude mouse colon cancer model, Ad5/F35-APE1 siRNA (5 Â 10 8 IU, intratumoral injection) inhibited the expression of APE1 protein in LOVO xenografts, and significantly enhanced inhibition of tumor growth by irradiation. In conclusion, APE1 may be involved as one of the radioresistance factors, and targeted inhibition of APE1 shows an effective means of enhancing tumor sensitivity to radiotherapy.

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Section: Western Blot Analysismentioning

confidence: 99%

Chimeric adenoviral vector Ad5/F35-mediated APE1 siRNA enhances sensitivity of human colorectal cancer cells to radiotherapy in vitro and in vivo

Wang

et al. 2008

Cancer Gene Ther

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“…Xiang et al [27] investigated the effect of CAPE on the growth, cell cycle and apoptosis of colon cancer cells and demonstrate that CAPE treatment was associated with a strong inhibition of growth in a doseand time-dependent manner, along with induction of G0/G1 arrest and apoptosis in both HCT116 and SW480 colon cancer cells. CAPE also reduced the expression of cyclin D1 and C-MYC in a dose and time dependent manner.…”

Section: Role Of Phenolic Acids On Colon Cancermentioning

confidence: 99%

Anticancer Properties of Phenolic Acids in Colon Cancer – A Review

LS¹,

NJA²

2016

J Nutr Food Sci

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Consumption of fruits and vegetables is associated with lower risk of several cancers, particularly colorectal cancer, which is mainly associated to their phytochemical content. A diverse range of phytochemicals, especially phenolic compounds, has been reported to possess important biological properties such as anticancer, antiviral, antioxidant and anti-inflammatory activities. Several factors contribute to the development of colorectal cancer. The scientific evidences support the genetic predisposition, diet, and lifestyle as some of the major contributing factors for colorectal cancer development. In this sense, this review aims to summarize the anticancer activities and the proposed mechanisms of action of phenolic acids with an emphasis in colon cancer through in vitro evidences. The evidences supports the theory of anticancer properties of phenolic acids, although the mechanisms are still not fully understood, but may include scavenging free radicals, induction of enzymes involved in the metabolism of xenobiotics, regulation of gene expression, modulation of cellular signaling pathways including those involved in DNA damage repair, cell proliferation, apoptosis and invasion.

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“…Among the more than 300 compounds identified in propolis samples (2), caffeic acid phenethyl ester (CAPE) inhibit the growth and induce G1-phase arrest and apoptosis in HCT116 and SW480 cells, by decreasing mRNA and protein expression of cyclin D1 and c-myc (33,34). In human HT1080 fibrosarcoma cells CAPE also decrease mRNA expression of matrix metalloproteinase (MMP) that plays a pivotal role in tumor metastasis by cleavage of extracellular matrix (35).…”

Section: Discussionmentioning

confidence: 99%

Growth inhibitory activity of ethanol extracts of Chinese and Brazilian propolis in four human colon carcinoma cell lines

Ishihara

Naoi²,

Hashita³

et al. 2009

Oncol Rep

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Abstract. More than 300 bio-active compounds have been identified from bee propolis in various regions of the world. The objective of this study was to examine whether the ethanol extracts of Chinese and Brazilian propolis may exert anticancer activities in four human colon carcinoma cell lines, namely CaCo2, HCT116, HT29 and SW480. Propolis samples were extracted with ethanol, and the crude extracts were dissolved in dimethylsulfoxide and used for the experiments. In HCT116, HT29 and SW480 cell lines, the extracts of both Chinese and Brazilian propolis caused a marked dose-dependent growth inhibition, with IC 50 values in the range of 4-41 μg/ml. In HCT116 cell line, Chinese propolis extract induced apoptosis in the cells after 72 h of treatment. In addition, Chinese propolis extract caused a dose-dependent increase in the cellular mRNA levels of p21 CIP1 and p53 in the HCT116 cell line. These findings indicate that the ethanol extracts of propolis contain components that may have anticancer activity. Thus, propolis and related products may provide a novel approach to the chemoprevention and treatment of human colon carcinoma. IntroductionThere is growing interest in the use of natural products to aid in the maintenance of human health. Natural products contain a wide variety of chemical compounds that have potent biological effects, including anticancer activity (1). Identification of the active components and their mechanism of action is important to assess their potential for clinical use and possible diverse side effects. Propolis is a natural product derived from various plant resins collected by honeybees, and has been used as a folk medicine for centuries in Europe, North and South America, China and Japan (2). Propolis has been reported to exhibit a broad spectrum of activities including antibacterial, antifungal, antiviral, anti-inflammatory, antioxidant, hepatoprotective, and anticancer properties (2,3). Propolis from Europe, North and South America, Asia and Africa differ in their composition because of the differences in the local vegetation (2,3). The medical application of propolis has led to increased interest in its chemical compositions and its botanical origins since polyphenol compounds and their derivatives have been identified in propolis (2,3).Colon carcinoma is a common malignancy ranking third in frequency on a world wide basis and causes about 500,000 deaths annually (4). This malignancy is most common in developed countries of Western world, and mortality rate of this disease is rising (4). It is thought that one-third of human carcinoma might be associated with dietary habits and lifestyle (5) and dietary factors are known to be critical modulators of the development of human colon carcinoma (6,7). Despite recent clinical advances, colon carcinoma still remains a major issue because of its incidence, morbidity, and mortality (4,8,9). One promising approach to reduce the incidence and improve the prognosis of this malignancy may be chemoprevention (1,10).Although the above-described fi...

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Caffeic acid phenethyl ester induces growth arrest and apoptosis of colon cancer cells via the β-catenin/T-cell factor signaling

Cited by 109 publications

References 42 publications

Chimeric adenoviral vector Ad5/F35-mediated APE1 siRNA enhances sensitivity of human colorectal cancer cells to radiotherapy in vitro and in vivo

Chimeric adenoviral vector Ad5/F35-mediated APE1 siRNA enhances sensitivity of human colorectal cancer cells to radiotherapy in vitro and in vivo

Anticancer Properties of Phenolic Acids in Colon Cancer – A Review

Growth inhibitory activity of ethanol extracts of Chinese and Brazilian propolis in four human colon carcinoma cell lines

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