Caffeine affects CD8+ lymphocyte apoptosis and migration differently in naïve and familiar individuals following moderate intensity exercise

Abstract

The purpose of this investigation was to determine the lymphocyte subset response to 30 min of moderate treadmill exercise during caffeine supplemented (6.0 mg.kg −1 ) and placebo conditions in caffeine-naïve and -familiar individuals. Seventeen individuals participated (caffeine-familiar = 8, caffeine-naïve = 9) completing two exercise bouts (caffeine supplemented and placebo control) 48 h apart in a counterbalanced and double-blinded fashion. Individuals were classified as follows: caffeine-naive <50 mg.d −1 and caffeine-familiar >200 mg.d −1 . Whole blood samples were obtained at rest, 30 min after caffeine or placebo ingestion, immediately following exercise, and 1 h post exercise. Blood was used to analyze apoptosis (annexin V) and cellular migration (CX 3 CR1) responses in lymphocyte subsets (CD4+, CD8+, CD19+). Absolute changes from rest values were calculated and differences between conditions were determined through Chisquared analysis with significance accepted at P <0.05. With regard to CD4+ and CD19+ lymphocytes, the interaction of caffeine and exercise did not affect naïve individuals to a greater extent immediately post exercise when compared to familiar, as similar apoptotic and migratory responses were observed (P >0.05). However, CD8+ lymphocyte cell death and migration responses were observed to be significantly greater at each sampling point in caffeine-familiar individuals (P <0.05). It is possible that chronic caffeine supplementation may prime CD8+ cell receptors for responsiveness to apoptosis and migration and the consequence of this form of immunosuppression in the post-exercise period should be determined.

Immunotropic effects of so-called slag metabolites (creatinine, urea, uric acid and bilirubin) at rats

“…It is known that the immunotropic effect of adenosine is realized through its receptors (A1, A2A, A2B, A3), which express virtually all populations of immunocytes: T, NK, B lymphocytes, macrophages, neutrophils, dendritic and endothelial cells [1,22,23,42]. Methylxanthines are nonselective antagonists of adenosine receptors, mainly A2A and A1 [8,28,29,30,35]. The facts obtained in our [19,20] previous human studies on the inversely relationship of uricemia (the level of which is comparable to the concentrations of adenosine and theophylline in immune tests in vitro) with a relative blood content of CD4 + CD3 + T-helper cells in combination with the presence of directly connection with the content of CD8 + CD3 + T-cytolytic cells indicate the similarity of the effects of uric acid with those of adenosine, ie activation of A2A receptors.…”

Relationships between parameters of uric acid exchange and immunity as well as microbiota in patients with neuroendocrine-immune complex dysfunction

“…In line with the concept we have previously adopted [7] that Uric acid is structural homolog of Caffeine, which in turn is a structural homolog of Adenosine [17,28], of particular interest are the data that peripheral T-cell depletion due to inhibition of T-cell expansion by extracellular Adenosine mediated signaling through A2A receptors [11]. It is shown that Caffeine increases cell death and migration in Cytotoxic but not Helper T-Lymphocytes as well as B-Lymphocytes in familiar but not naїve individuals following moderate intensity exercise [18].…”

Relationships between changes in uric acid parameters metabolism and parameters of immunity and microbiota in patients with neuroendocrine-immune complex dysfunction

“…A non-selective Adenosine receptor antagonists, mainly A2A, are caffeine (2,6-dioxi-1,3,7trimethylpurine or 1,3,7-trimethylxantine) and other methylxanthines [20,21] which are introduced into the human body almost daily from coffee, tea and cocoa.…”