CagA status and VacA subtypes of Helicobacter pylori in relation to histopathologic findings in Iranian population

Molaei, Mahsa; Forough, Foroughi; Mashayekhi, Reza; Haghazali, Mehrdad; Zojaji, Homayoun; Jafari, Fatemeh; Dabiri, Hossein; Zali, Mohammad Reza

doi:10.4103/0377-4929.59178

Cited by 19 publications

(12 citation statements)

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“…In four studies carried out in Iran the prevalence of s1 allele as the most common allele of VacA cytotoxin subtypes was found to be 71%, 69%, 69% and 70.5%, respectively and is in accordance with our study. [30313233] But contrary to our study, s2 allele was present in all these studies. The dominant genotype in Jafari, Dabiri and Molaei studies was vacA s1/m2, whereas in Isfahan this prevalence was 51% for vacA s1/m1 and 49% for s1/m2.…”

Section: Discussioncontrasting

confidence: 99%

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Prevalence of Helicobacter pylori vacA different genotypes in Isfahan, Iran

et al. 2014

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Background:It is believed that the Helicobacter pylori (H. pylori) vacA gene, as a major virulence determinant (One of the major virulence determinant, not major), may be a risk factor for the development of gastroduodenal diseases. The frequency of vacA genotypes varies in different human populations. This study evaluated the prevalence of vacA alleles/genotypes among dyspeptic patients in Isfahan.Materials and Methods:One-hundred H. pylori-positive adult patients were examined in this study. After culture of gastric biopsies and DNA extraction from individual H. pylori isolates, the (all H. pylori strains harbor vacA alleles, please replace “presence” with “genotypes”) of the vacA s and m alleles were determined using polymerase chain reaction (PCR).Results:There were four vacA mosaicisms, including 28 for s1a/m1 (28%), 23 for s1b/m1 (23%), 26 for s1a/m2 (26%) and 23 for s1b/m2 (23%). The s2 allele was not found. The predominant vacA genotype in patients with non-ulcer dyspepsia and duodenal ulcer was s1a/m2, whereas in patients with adenocarcinoma was s1a/m1.Conclusion:The results showed there was no significant correlation between different genotypes of the vacA and the clinical outcomes and appears to vacA genotypes were not useful determinants for gastrointestinal diseases in our area.

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Section: Discussioncontrasting

confidence: 99%

“…These results were also reported in the previous studies carried out in Iran, Taiwan and Mexico. In the studies conducted by Yakoob, et al .,[16] Molaei, et al .,[33] Chiarini, et al .,[38] Bindayna and Mahmeed[36] significant association was found between vacA genotypes and clinical outcomes. Hussein et al .…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Helicobacter pylori vacA different genotypes in Isfahan, Iran

et al. 2014

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“…We found that prevalence of cagA was 77.27% (17/22), which was in conformity to various studies done where the prevalence varies from 62%-77% respectively [12,13], while in one study done by Udhayakumar G et al, prevalence of cagA positive strain was 96% [14]. The prevalence of cagA genotypes in Anand district is relatively important to reveal the circulating H. pylori strains in a given geographic region and the associated need for regionspecific diagnostics for H. pylori virulence markers.…”

Section: Resultssupporting

confidence: 70%

Prevalence of Specific Helicobacter Pylori CagA, VacA, IceA, UreC Genotypes and its Clinical Relevance in the Patients with Acid-Peptic Diseases

Pandya¹,

Agravat

Patel

2017

JCDR

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Introduction: Virulent markers of H. pylori, the vacuolating cytotoxin (vacA), cytotoxin-associated gene A (cagA), induced by contact with epithelium factor antigen (iceA gene) and the urease C gene (ureC) may plays a major role in determining the clinical outcome of Helicobacter infections. Aim:To detect the prevalence of the cagA, vacA, ureC and iceA genotypes of H. pylori from antral biopsy specimens of patients and to associate its role in specific disease. Materials and Methods:The study was conducted at Department of Microbiology of Shree P.M. Patel College of Paramedical Sciences, Anand, Gujarat, India. Seventy one antral biopsies of symptomatic patients referred for endoscopy from October 2012 to September 2013 were subjected to Multiplex PCR. DNA isolation from 71 biopsy samples was done by using "QIAamp DNA mini kit" from QIAGEN (GmbH, Hilden, Germany). Data was analysed using Chi square (χ 2 ) test and p-value<0.05 was considered significant. Results:Out of the 71 biopsies screened, 22(31%) samples were positive for H. pylori by PCR, with high proportion of cagA positive (17/22 specimen; 77.27%), followed by ureC positive (4/22 specimen; 18.18%) and vacA positive (1/22 specimen; 4.54%) strains. Significant association was found between cagA and female gender (p-value=0.042). Out of 17 cagA positive strains, 9(52.94%) were found in patients with gastritis, 5(29.41%) in reflux oesophagitis and 3(17.64%) in patients with diodenal ulcer. We found 0% prevalence of iceA gene; conversely we had three peptic ulcer patients with only cagA positivity. Conclusion:The cagA positive strain mainly affects the patients with gastritis specifically of female gender and iceA genotype is not a useful marker associated with peptic ulcer disease. Patients should be screened for cagA genotype when reported to be a case of gastritis for early treatment to prevent further complications such as cancer.

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“…While a great number of studies in Iran indicated no influence of cagA status or its 3' variable region on the progression of disease (26)(27)(28)(29)(30)(31)(32)(33)(34), correlation between positive cagA status and severe clinical outcomes was reported in some other studies (35)(36)(37). Consistent with the cases of Iranian adult populations (27,28,(38)(39)(40)(41), vacA s1 was the most prevalent in the present investigation (Table 3). This dominance was also consistent with the cases of other countries in the northern part of the Middle East (42,43).…”

Section: Discussionmentioning

confidence: 99%

Analysis of vacA/cagA genotypes/status in Helicobacter pylori isolates from Iranian children and their association with clinical outcome

Falsafi¹,

Khani²,

Mahjoub³

et al. 2015

Turk J Med Sci

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Background/aim: More than 50% of Iranian children are infected with Helicobacter pylori; however, no data exist about the association of vacA/cagA genotype/status with disease outcomes in them. We analyzed association of vacA/cagA genotypes/status of children's isolates with gastric inflammation status as the first step in H. pylori pathogenesis. Materials and methods:Antral biopsies for culture and histopathology were taken from 328 children in 1997-2009. vacA (s, m) alleles and cagA statuses of the isolates were determined by PCR. Histopathology was performed according to the Sydney system; gastritis was scored as normal, mild, moderate, severe, and follicular.Results: A total of 159 culture-positive cases, with no mixed infections, were enrolled in the study. Of them, 60% were cagA-positive; 21.4%, 37.1%, 16.3%, and 25.2% cases were s1m1, s1m2, s2m1, and s2m2, respectively. Histopathology showed normal (4.4%), mildchronic (31.4%), moderate-chronic (38.4%), severe-chronic (10.7%), and follicular gastritis (15.1%) cases. Thirty-four (21.4%) of the children had ulcers. Correlation (P < 0.05) was observed between more severe (moderate, severe, follicular) status and both vacAs1 allele and cagA-positive status. No significant relation was observed between genotype/status of vacA/cagA and ulcers (P > 0.05). Conclusion: vacAs1and cagA are associated with more severe gastric inflammation in Iranian children. Association of vacAs1 and cagA with more severe pathology in Iran may be similar to that of other parts of the world.

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CagA status and VacA subtypes of Helicobacter pylori in relation to histopathologic findings in Iranian population

Abstract: H. pylori strains showing vacA s1 genotype were associated with more severe gastritis. These findings show that vacA genotyping may have clinical relevance in Iran.

Cited by 19 publications

References 4 publications

Prevalence of Helicobacter pylori vacA different genotypes in Isfahan, Iran

Prevalence of Helicobacter pylori vacA different genotypes in Isfahan, Iran

Prevalence of Specific Helicobacter Pylori CagA, VacA, IceA, UreC Genotypes and its Clinical Relevance in the Patients with Acid-Peptic Diseases

Analysis of vacA/cagA genotypes/status in Helicobacter pylori isolates from Iranian children and their association with clinical outcome

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