Caipira sweet orange + Rangpur lime: a somatic hybrid with potential for use as rootstock in the Brazilian citrus industry

Mendes-da-Glória, Fernanda Januzzi; Filho, Francisco de Assis Alves Mourão; Camargo, Luís Eduardo Aranha; Mendes, Beatriz Madalena Januzzi

doi:10.1590/s1415-47572000000300026

Cited by 25 publications

(10 citation statements)

References 23 publications

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“…Biotechnological techniques such as cell and tissue cultures and molecular biology have helped breeders to overcome these difficulties. In addition, the hybridization through protoplast fusion and genetic transformation may contribute significantly to avoid these limitations (Mendes-da-Glória et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium-mediated transformation of Citrus sinensis and Citrus limonia epicotyl segments

Almeida

Filho

Mendes

et al. 2003

Sci. agric. (Piracicaba, Braz.)

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Genetic transformation allows the release of improved cultivars with desirable characteristics in a shorter period of time and therefore may be useful in citrus breeding programs. The objective of this research was to establish a protocol for genetic transformation of Valencia and Natal sweet oranges (Citrus sinensis L. Osbeck) and Rangpur lime (Citrus limonia L. Osbeck). Epicotyl segments of germinated in vitro plantlets (three weeks in darkness and two weeks in a 16-h photoperiod) were used as explants. These were cocultivated with Agrobacterium tumefaciens strain EHA-105 and different experiments were done to evaluate the transformation efficiency: explants were co-cultivated with Agrobacterium for one, three or five days; explants were incubated with Agrobacterium suspension for 5, 10, 20 or 40 minutes; co-cultivation medium was supplemented with acetosyringone at 0, 100 or 200 µmol L -1; Explants ends had a longitudinal terminal incision (2-3 mm); co-cultivation temperatures of 19, 23 or 27°C were imposed. The experimental design was completely randomized in all experiments with five replications, each consisted of a Petri dish (100 x 15 mm) with 30 explants and resulted in a total of 150 explants per treatment. Longitudinal terminal incision in the explant ends did not improve shoot regeneration. However, transgenic plants of all three cultivars were confirmed from explants that had been subjected to inoculation time of 20 minutes, co-culture of three days at 23-27°C, in the absence of acetosyringone. Key words: genetic transformation, organogenesis, micrografting, improvement TRANSFORMAÇÃO GENÉTICA EM Citrus sinensis E Citrus limonia MEDIADA POR Agrobacterium tumefaciens A PARTIR DE SEGMENTOS DE EPICÓTILORESUMO: A transformação genética permite produzir cultivares com características específicas e pode, dessa forma ser associada a programas de melhoramento de citros. O objetivo deste trabalho foi estabelecer protocolos de transformação genética para as laranjas doce 'Valência' e 'Natal' (Citrus sinensis L. Osbeck), bem como para o limão 'Cravo'(Citrus limonia L. Osbeck). Segmentos de epicótilo de plântulas germinadas in vitro (três semanas no escuro e duas semanas sob fotoperíodo de 16h) foram utilizados como explantes. Estes foram co-cultivados com Agrobacterium tumefaciens (EHA-105), realizando-se vários experimentos para avaliar a eficiência do processo de transformação genética: explantes co-cultivados por um, três e cinco dias; tempo de inoculação com a bactéria de 5, 10, 20 e 40 minutos; co-cultivo em meio de cultura contendo 0, 100 e 200 µmol L -1 de acetoseringona; Incisão longitudinal (2-3 mm) nas extremidades do explante; temperatura de co-cultivo 19, 23 e 27°C. Todos os experimentos consistiram de cinco repetições por tratamento, sendo cada repetição representada por uma placa de Petri contendo 30 explantes, perfazendo um total de 150 explantes por tratamento. Plântulas transgênicas dos três cultivares foram obtidas utilizando-se tempo de inoculação de 20 minutos, co-cultivo com Agrob...

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Section: Introductionmentioning

confidence: 99%

Agrobacterium-mediated transformation of Citrus sinensis and Citrus limonia epicotyl segments

Almeida

Filho

Mendes

et al. 2003

Sci. agric. (Piracicaba, Braz.)

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“…In Brazil, citrus protoplast fusion approaches have been developed since 1995, leading to the production of somatic hybrids for rootstock improvement aiming at the control of important diseases, such as blight and citrus tristeza virus (CTV) (Mendes-da-Glória et al, 2000;Mendes et al, 2001;Latado et al, 2002;Costa et al, 2003).…”

Section: Hibridação Somática Entre Citrus Sinensis (L) Osbeck E C Gmentioning

confidence: 99%

Somatic hybridization between Citrus sinensis (L.) Osbeck and C. grandis (L.) Osbeck

Calixto

Filho

Mendes

et al. 2004

Pesq. agropec. bras.

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This work had as objective to produce citrus somatic hybrids between sweet oranges and pummelos. After chemical fusion of sweet orange embryogenic protoplasts with pummelo mesophyll-derived protoplasts, plants were regenerated by somatic embryogenesis and acclimatized in a greenhouse. The hybrids of 'Hamlin' sweet orange + 'Indian Red' pummelo and 'Hamlin' sweet orange + 'Singapura' pummelo were confirmed by leaf morphology, chromosome counting and molecular analysis. These hybrids have potential to be used directly as rootstocks aiming blight, citrus tristeza virus, and Phytophthora-induced disease tolerance, as well as for rootstocks improvement programs.

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“…Desde então, outros trabalhos foram realizados, com o objetivo de produzir híbridos somáticos interespecíficos Grosser et al, 1998a;Mendes-da-Glória et al, 2000;Mendes et al, 2001), híbridos somáticos intergenéricos com parentais sexualmente compatíveis (Ohgawara, 1985, Grosser et al, 1988aGrosser et al 1996;Louzada et al, 1992) e os híbridos intergenéricos de Citrus com parentais sexualmente incompatíveis (Grosser et al 1988b;1996;Motomura et al, 1995), de variedades copa e porta-enxerto com o intuito de aproveitar características relacionadas a resistência ao frio, tolerância a solo salino e resistência a doenças. Kobayashi et al (1991) relataram que híbridos somáticos em condições de campo apresentam bom desenvolvimento com fertilidade de óvulo e pólen.…”

Section: Hibridação Somáticaunclassified

Estudo de fatores que influenciam o processo de transformação genética em citros via Agrobacterium tumefaciens.

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Caipira sweet orange + Rangpur lime: a somatic hybrid with potential for use as rootstock in the Brazilian citrus industry

Cited by 25 publications

References 23 publications

Agrobacterium-mediated transformation of Citrus sinensis and Citrus limonia epicotyl segments

Agrobacterium-mediated transformation of Citrus sinensis and Citrus limonia epicotyl segments

Somatic hybridization between Citrus sinensis (L.) Osbeck and C. grandis (L.) Osbeck

Estudo de fatores que influenciam o processo de transformação genética em citros via Agrobacterium tumefaciens.

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