Calcineurin-Responsive Transcription Factor CgCrzA Is Required for Cell Wall Integrity and Infection-Related Morphogenesis in Colletotrichum gloeosporioides

Wang, Ping; Li, Bing; Pan, Yuting; Zhang, Yun-Zhao; Li, Dewei; Huang, Lin

doi:10.5423/ppj.oa.04.2020.0071

Cited by 18 publications

(27 citation statements)

References 54 publications

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“…CRZ1 and CMK1 act as regulators of the upstream signals to control the growth, conidiation, invasive structures development, and pathogenicity in fungal pathogens ( Mey et al, 2002 ; Wang et al, 2020 ). Here, heterologous expression of CgNPG1 in C. acutatum Hb obviously upregulated the transcription of CaCRZ1 and CaCMK1 , with the expression level of CaCRZ1 increased to twofold and CaCMK1 to 1.3-fold ( Figure 8 ), indicating that the effect of CgNPG1 on C. acutatum Hb might be related to the modulation of CaCRZ1 and CaCMK1.…”

Section: Resultsmentioning

confidence: 99%

“…As a major component of Ca 2+ signaling pathway, MoCRZ1 was involved in growth, conidiation, and pathogenicity ( Choi et al, 2009 ). As CRZ1 homolog in C. gloeosporioides s.s. strain SMCG1#C, CgCrzA was also involved in vegetative growth, conidiation, appressorial formation rate, and lost pathogenicity to plant hosts ( Wang et al, 2020 ). In addition, MAP kinase cascades are also an important regulatory system in cells ( Dean, 1997 ).…”

Section: Discussionmentioning

confidence: 99%

“…In C. gloeosporioides , CgCrzA as the homolog of CRZ1, was involved in regulating cell wall integrity and infection-related morphogenesis. Deletion of the CgCRZA in C. gloeosporioides showed severe cell wall integrity defects and inhibited the vegetative growth, conidiation, appressorial formation rate, and lost pathogenicity to plant hosts ( Wang et al, 2020 ). These data suggested that both CMK1 and CRZ1 in Colletotrichum were involved in fungal development and pathogenesis.…”

Section: Introductionmentioning

confidence: 99%

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CgNPG1 as a Novel Pathogenic Gene of Colletotrichum gloeosporioides From Hevea brasiliensis in Mycelial Growth, Conidiation, and the Invasive Structures Development

et al. 2021

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The rubber tree (Hevea brasiliensis) is a tropical perennial crop for the primary source of natural rubber. Colletotrichum gloeosporioides from Hevea brasiliensis (C. gloeosporioides Hb) and Colletotrichum acutatum from Hevea brasiliensis (C. acutatum Hb) are the causal agents of rubber tree anthracnose and lead to serious loss of natural rubber production. Inoculation tests showed that C. gloeosporioides Hb possessed higher pathogenicity than C. acutatum Hb to the rubber tree. Genomic analysis revealed that an unknown gene, named CgNPG1 (a Novel Pathogenic Gene 1), was presented in the genome of C. gloeosporioides Hb but not identified in C. acutatum Hb. CgNPG1 was predicted to encode a small secretory protein without any conserved domain. To investigate the functions of CgNPG1 in C. gloeosporioides Hb and in C. acutatum Hb, the gene deletion and overexpression mutants were generated. The phenotype analysis showed that deletion of CgNPG1 led to changed conidia morphology, decreased mycelial growth, conidiation, conidia germination rate, appressorium formation rate, and pathogenicity of C. gloeosporioides Hb to the rubber tree. Meanwhile, heterogeneous expression of CgNPG1 in C. acutatum Hb significantly changed the conidia morphology and improved the mycelial growth rate, conidiation, conidia germination rate, appressorium formation rate, and the pathogenicity of C. acutatum Hb to the rubber tree. Consistently, CgNPG1 increased the expression level of CaCRZ1 and CaCMK1 in C. acutatum Hb. These data suggested that CgNPG1 contributed to mycelial growth, conidiation, the development of invasive structures, and the pathogenicity of Colletotrichum to the rubber tree, which might be related to the modulation of CaCRZ1 and mitogen-activated protein kinase CMK1. Our results provided new insight into CgNPG1 in regulating growth and pathogenicity of the Colletotrichum spp.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

CgNPG1 as a Novel Pathogenic Gene of Colletotrichum gloeosporioides From Hevea brasiliensis in Mycelial Growth, Conidiation, and the Invasive Structures Development

et al. 2021

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“…Moreover, our study demonstrated the procedure for constructing a three‐fragment mutagenesis cassette with high‐GC content, which required overlap extension PCR combined with the touchdown PCR. Although overlap extension PCR is commonly used to construct three‐fragment mutagenesis cassettes (Wang et al., 2020), the amplification was hampered by the size and GC content of target DNA (Kang et al., 2011). This may be one of the reasons why there are few reports on the knockout of highly GC‐rich genes.…”

Section: Discussionmentioning

confidence: 99%

“…Generally, linear fragment‐based mutagenesis is commonly used to manipulate genes in HR (Fang et al., 2018; Murphy et al., 2000; Wang et al., 2020; Yu et al., 2004). However, the results presented here demonstrated that linear fragment‐based mutagenesis was not suitable for gene‐editing of B. pyrrocinia JK‐SH007.…”

Section: Discussionmentioning

confidence: 99%

Knockout of a highly GC‐rich gene in Burkholderia pyrrocinia by recombineering with freeze‐thawing transformation

Chen

Liu

et al. 2021

Molecular Plant Pathology

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The ability to manipulate the bacterial chromosome in molecular genetics and genetic engineering studies can provide significant insight into gene function (Fan et al., 2016;Ito et al., 2005). In vivo analysis of gene function and construction of the engineered bacteria require reliable genetic transformation (Cao et al., 2017;Matheka et al., 2019;Riglar & Silver, 2018). An optimal genetic transformation system generally includes an efficient transformation system, a reliable gene-editing system, and a simplified screening method (Niyomtham

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Gcc1 homologs regulate growth, oxidative stress, conidiation and appressorium formation in Colletotrichum siamense and Colletotrichum graminicola

Wang

Zhang

Zhou

et al. 2023

Microbial Pathogenesis

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Calcineurin-Responsive Transcription Factor CgCrzA Is Required for Cell Wall Integrity and Infection-Related Morphogenesis in Colletotrichum gloeosporioides

Cited by 18 publications

References 54 publications

CgNPG1 as a Novel Pathogenic Gene of Colletotrichum gloeosporioides From Hevea brasiliensis in Mycelial Growth, Conidiation, and the Invasive Structures Development

CgNPG1 as a Novel Pathogenic Gene of Colletotrichum gloeosporioides From Hevea brasiliensis in Mycelial Growth, Conidiation, and the Invasive Structures Development

Knockout of a highly GC‐rich gene in Burkholderia pyrrocinia by recombineering with freeze‐thawing transformation

Gcc1 homologs regulate growth, oxidative stress, conidiation and appressorium formation in Colletotrichum siamense and Colletotrichum graminicola

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