Calcite precipitation induced by Bacillus cereus MRR2 cultured at different Ca2+ concentrations: Further insights into biotic and abiotic calcite

Zhuang, Dingxiang; Yan, Huaxiao; Tucker, Maurice E.; Zhao, Hui; Han, Zuozhen; Zhao, Yue; Sun, Bin; Li, Dan; Pan, Juntong; Zhao, Yanyang; Meng, Ruirui; Shan, Guanghe; Zhang, Xinkang; Tang, Rongzhen

doi:10.1016/j.chemgeo.2018.09.018

Cited by 108 publications

(123 citation statements)

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“…The adsorption peaks at 712, 875 and 1421 cm −1 are the characteristic peaks of calcite, and the characteristic peaks of vaterite are at 745, 870 and 1421 cm −1 [2,7]. In addition, the peaks at 700, 765, 872, 1064, 1401 and 1480 cm −1 record the presence of monohydrocalcite [7,58].…”

Section: Ftir Analysesmentioning

confidence: 96%

“…The cell concentration of the liquid medium in the experimental group was measured using a spectrophotometer (UNIC7200, Shanghai Sainty Hengfeng Scientific Instrument Co. Ltd., Shanghai, China); at the same time, pH was also measured with a pH meter (PHS-3E, Jiangfen Electrical Analysis Instrument Co. Ltd., Taizhou, China). An ammonia test was performed according to the published method [7]. CA activity and concentrations of carbonate and bicarbonate ions were measured with the methods described in a previous paper [7].…”

Section: Biochemical Parameters Of Liquid Medium Inoculated By C Frementioning

confidence: 99%

“…An ammonia test was performed according to the published method [7]. CA activity and concentrations of carbonate and bicarbonate ions were measured with the methods described in a previous paper [7]. ALP activity was measured using the method described in [34].…”

Section: Biochemical Parameters Of Liquid Medium Inoculated By C Frementioning

confidence: 99%

“…Microorganisms, especially bacteria, as an important part of the Earth's biosphere, can facilitate many geochemical processes. Extensive research has been undertaken on the biomineralization processes of carbonate and phosphate minerals in the past decades, and the significant roles that microorganisms play in these processes have been widely reported [1][2][3][4][5][6][7][8][9][10][11]. Many species of microorganisms have been used to induce the precipitation of carbonate and phosphate minerals, including cyanobacteria, halophiles, methanogens and sulfate-reducing bacteria [8,[11][12][13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

“…strain on the precipitation of calcite, and they found that ammonia released by the bacteria played a significant role in the observed pH increase due to the fact that the hydration of ammonia can produce hydroxyl ions, resulting in an increase in alkalinity [23]. However, Zhuang et al found that pH still increased when the concentration of ammonia had reached a plateau; they proposed that the further pH increase resulted from the combined effect of ammonia and carbonic anhydrase (CA) [7]. Secondly, many researchers have reported that the cell wall itself and EPS can act as nucleation sites [24][25][26].…”

Section: Introductionmentioning

confidence: 99%

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Bio-Precipitation of Carbonate and Phosphate Minerals Induced by the Bacterium Citrobacter freundii ZW123 in an Anaerobic Environment

Sun

Zhao

et al. 2020

Minerals

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In this study, a facultative anaerobic strain isolated from marine sediments and identified as Citrobacter freundii, was used to induce the precipitation of carbonate and phosphate minerals in the laboratory under anaerobic conditions. This is the first time that the ability of C. freundii ZW123 to precipitate carbonate and phosphate minerals has been demonstrated. During the experiments, carbonic anhydrase, alkaline phosphatase and ammonium released by the bacteria not only promoted an increase in pH, but also drove the supersaturation and precipitation of carbonate and phosphate minerals. The predominant bio-mediated minerals precipitated at various Mg/Ca molar ratios were calcite, vaterite, Mg-rich calcite, monohydrocalcite and struvite. A preferred orientation towards struvite was observed. Scanning transmission electron microscopy (STEM) and elemental mapping showed the distribution of magnesium and calcium elements within Mg-rich calcite. Many organic functional groups, including C=O, C–O–C and C–O, were detected within the biominerals, and these functional groups were also identified in the associated extracellular polymeric substances (EPS). Fifteen kinds of amino acid were detected in the biotic minerals, almost identical to those of the EPS, indicating a close relationship between EPS and biominerals. Most amino acids are negatively charged and able to adsorb cations, providing an oversaturated microenvironment to facilitate mineral nucleation. The X-ray photoelectron spectroscopy (XPS) spectrum of struvite shows the presence of organic functional groups on the mineral surface, suggesting a role of the microorganism in struvite precipitation. The ZW123 bacteria provided carbon and nitrogen for the formation of the biotic minerals through their metabolism, which further emphasizes the close relationship between biominerals and the microorganisms. Thermal studies showed the enhanced thermal stability of biotic minerals, perhaps due to the participation of the bacteria ZW123. The presence of amino acids such as Asp and Glu may explain the high magnesium content of some calcites. Molecular dynamics simulations demonstrated that the morphological change and preferred orientation were likely caused by selective adsorption of EPS onto the various struvite crystal surfaces. Thus, this study shows the significant role played by C. freundii ZW123 in the bioprecipitation of carbonate and phosphate minerals and provides some insights into the processes involved.

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