Calcium-binding proteins expression in the septum and cingulate cortex of the adult guinea pig

Hermanowicz-Sobieraj, Beata; Bogus‐Nowakowska, Krystyna; Robak, Anna

doi:10.1016/j.aanat.2017.09.009

Cited by 12 publications

(4 citation statements)

References 83 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Real‐time PCR analysis was carried out on a 7500 Fast real‐time PCR system (Applied Biosystems, USA) as described previously (Hermanowicz‐Sobieraj et al ., ). The real‐time PCR reaction included 1 μl cDNA (100 ng), 0.2 μl reference dye, and 1μl (5 micro molar concentration) of each primer (reverse and forward, Sigma‐Aldrich), 10 μl SYBR Green JumpStart TaqReadyMix, and RNase free water to a total volume of 20 μl.…”

Section: Methodsmentioning

confidence: 97%

“…The specificity of the primary antisera used in the present study has been confirmed by the manufacturers and by numerous researchers in various previous papers (Zimmermann and Schwaller, ; Drexel et al ., ; Mészár et al ., ; Berg et al ., ) including our team (Najdzion et al ., ; Zakowski et al ., ; Hermanowicz‐Sobieraj et al ., ; Równiak et al ., ). The rabbit anti‐CB (CB‐38), mouse anti‐CB (300), and mouse anti‐CR (6B3) antisera include immunoblots of the guinea pig brain homogenates, which were specifically stained by these antibodies, exhibiting bands at 28, 28, and 29 kDa, respectively (manufacturer's technical information).…”

Section: Methodsmentioning

confidence: 99%

“…Moreover, these proteins may have an impact on growth cone motility, neurite sprouting, elongation and branching as well as on neuronal cell proliferation, programmed cell death and gene expression (Mattson et al ., ; Mattson, ; Stuart and Lephart, ; Fields et al ., ). Even though the exact physiological function of CB, CR, and PV is still not completely understood, they have been demonstrated to be important markers of separate neuronal populations in the adult brain (Arai et al ., ; Baimbridge et al ., ; Andressen et al ., ; Najdzion et al ., ; Równiak et al ., ; Wasilewska et al ., ; Hermanowicz‐Sobieraj et al ., ) and during brain ontogeny (Solbach and Celio, ; Zakowski and Robak, 2013; Hermanowicz‐Sobieraj and Robak, ). In the guinea pig, these proteins mark the vast majority of GABAergic neurons in amygdala, septum, and subicular complex (Równiak et al ., ; Wasilewska et al ., ; Hermanowicz‐Sobieraj and Robak, ; Równiak, ).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Ontogeny of Neurons Containing Calcium‐Binding Proteins in the Preoptic Area of the Guinea Pig: Sexually Dimorphic Development of Calbindin

Bogus‐Nowakowska

2019

Developmental Neurobiology

View full text Add to dashboard Cite

This study characterizes for the first time the distribution and coexistence patterns of calbindin (CB), calretinin (CR), and parvalbumin (PV) in the female and male guinea pig preoptic area (POA) during brain development, using immunohistochemistry and quantitative real‐time PCR techniques. The results show that the prenatal development of the guinea pig POA takes place in elevated levels of CB and CR immunoreactivity with the peak at embryonic day 50 (E50) and generally in newborns both these proteins reach an adult‐like pattern of immunoreactivity, contrary to PV which appears later, peaks at postnatal day (PND) 10 (P10), and stabilizes at P20. CB and CR have also overlapping distributions which differed from that of PV, and much higher expressions at mRNA and protein levels. However, CB‐positive (+), CR+ and PV+ neurons create in the guinea pig POA separate populations as CB and CR coexisted only in a small number of neurons and CB+ cells never coexpressed PV. Moreover, the density of CB+ neurons, contrary to CR+ and PV+ cells, is sexually dimorphic favoring males at all the examined stages. In conclusion, elevated levels of CR and CB at the time of intense cell migration, differentiation, myelination, and synaptogenesis in the guinea pig brain suggest that these proteins may be engaged in similar processes in the POA, while late onset of PV may be rather linked with POA maturation. As the population of CB+ cells in the POA is very large, its dimorphic development may have huge impact on the sexual differentiation of this brain region.

show abstract

Section: Methodsmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ontogeny of Neurons Containing Calcium‐Binding Proteins in the Preoptic Area of the Guinea Pig: Sexually Dimorphic Development of Calbindin

Bogus‐Nowakowska

2019

Developmental Neurobiology

View full text Add to dashboard Cite

show abstract

“…The specificity of the primary antibodies directed against CB, CR and PV has been shown by various researchers using these products in numerous previous studies [108][109][110][111] including our team [4,15,72,112,113]. Moreover, the mouse anti-CB (300) and mouse anti-CR (6B3) antisera included immunoblots of the guinea pig brain homogenates, which were specifically stained by these antibodies, exhibiting bands at 28 and 29 kDa, respectively (manufacturer's technical information).…”

Section: Controlsmentioning

confidence: 94%

GABAergic and Glutamatergic Phenotypes of Neurons Expressing Calcium-Binding Proteins in the Preoptic Area of the Guinea Pig

Bogus‐Nowakowska

Robak

Kalinowski

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

The mammalian preoptic area (POA) has large populations of calbindin (CB), calretinin (CR) and parvalbumin (PV) neurons, but phenotypes of these cells are unknown. Therefore, the question is whether neurons expressing CB, CR, and/or PV are GABAergic or glutamatergic. Double-immunofluorescence staining followed by epifluorescence and confocal microscopy was used to determine the coexpression patterns of CB, CR and PV expressing neurons with vesicular GABA transporters (VGAT) as specific markers of GABAergic neurons and vesicular glutamate transporters (VGLUT 2) as specific markers of glutamatergic neurons. The guinea pig was adopted as, like humans, it has a reproductive cycle with a true luteal phase and a long gestation period. The results demonstrated that in the guinea pig POA of both sexes, ~80% of CB+ and ~90% of CR+ neurons coexpress VGAT; however, one-fifth of CB+ neurons and one-third of CR+ cells coexpress VGLUT. About two-thirds of PV+ neurons express VGAT, and similar proportion of them coexpress VGLUT. Thus, many CB+, CR+ and PV+ neurons may be exclusively GABAergic (VGAT-expressing cells) or glutamatergic (VGLUT-expressing cells); however, at least a small fraction of CR+ cells and at least one-third of PV+ cells are likely neurons with a dual GABA/glutamate phenotype that may coexpress both transporters.

show abstract

Immunohistological study of the unexplored vomeronasal organ of an endangered mammal, the dama gazelle (Nanger dama)

Torres

Ortiz‐Leal

Ferreiro³

et al. 2023

Microscopy Res & Technique

View full text Add to dashboard Cite

Dama gazelle is a threatened and rarely studied species found primarily in northern Africa. Human pressure has depleted the dama gazelle population from tens of thousands to a few hundred individuals. Since 1970, a founder population consisting of the last 17 surviving individuals in Western Sahara has been maintained in captivity, reproducing naturally. In preparation for the future implementation of assisted reproductive technology, certain aspects of dama gazelle reproductive biology have been established. However, the role played by semiochemical‐mediated communications in the sexual behavior of dama gazelle remains unknown due partially to a lack of a neuroanatomical or morphofunctional characterization of the dama gazelle vomeronasal organ (VNO), which is the sensory organ responsible for pheromone processing. The present study characterized the dama gazelle VNO, which appears fully equipped to perform neurosensory functions, contributing to current understanding of interspecies VNO variability among ruminants. By employing histological, lectin‐histochemical, and immunohistochemical techniques, we conducted a detailed morphofunctional evaluation of the dama gazelle VNO along its entire longitudinal axis. Our findings of significant structural and neurochemical transformation along the entire VNO suggest that future studies of the VNO should take a similar approach. The present study contributes to current understanding of dama gazelle VNO, providing a basis for future studies of semiochemical‐mediated communications and reproductive management in this species.Research Highlights This exhaustive immunohistological study of the vomeronasal organ (VNO) of the dama gazelle provides the first evidence of notable differences in the expression of neuronal markers along the rostrocaudal axis of the VNO. This provides a morphological basis for the implementation of pheromones in captive populations of dama gazelle.

show abstract

Calcium-binding proteins expression in the septum and cingulate cortex of the adult guinea pig

Cited by 12 publications

References 83 publications

Ontogeny of Neurons Containing Calcium‐Binding Proteins in the Preoptic Area of the Guinea Pig: Sexually Dimorphic Development of Calbindin

Ontogeny of Neurons Containing Calcium‐Binding Proteins in the Preoptic Area of the Guinea Pig: Sexually Dimorphic Development of Calbindin

GABAergic and Glutamatergic Phenotypes of Neurons Expressing Calcium-Binding Proteins in the Preoptic Area of the Guinea Pig

Immunohistological study of the unexplored vomeronasal organ of an endangered mammal, the dama gazelle (Nanger dama)

Contact Info

Product

Resources

About