Calcium Carbonate Mineralization: Involvement of Extracellular Polymeric Materials Isolated from Calcifying Bacteria

Ercole, Claudia; Bozzelli, Paola; Altieri, Fabio; Cacchio, Paola; Gallo, Maddalena Del

doi:10.1017/s1431927612000426

Cited by 60 publications

(43 citation statements)

References 53 publications

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“…Extracellular polymeric substances (proteins and/or polysaccharides) can play an important role in biomineralization, regulating and controlling nucleation, and growth of crystal structures (Ercole et al 2012;Kawaguchi and Decho 2002;Li and Gadd 2017a, b;Perri et al 2018;Tourney and Ngwenya 2014). In our study, it was found that the supernatants, or EPS obtained from supernatants, did not exert a significant influence on crystal morphology compared with chemical methods during the formation of cobalt and nickel oxalate.…”

Section: Discussionmentioning

confidence: 58%

Biorecovery of cobalt and nickel using biomass-free culture supernatants from Aspergillus niger

Yang

Song

Ferrier

et al. 2019

Appl Microbiol Biotechnol

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In this research, the capabilities of culture supernatants generated by the oxalate-producing fungus Aspergillus niger for the bioprecipitation and biorecovery of cobalt and nickel were investigated, as was the influence of extracellular polymeric substances (EPS) on these processes. The removal of cobalt from solution was >90% for all tested Co concentrations: maximal nickel recovery was >80%. Energy-dispersive X-ray analysis (EDXA) and X-ray diffraction (XRD) confirmed the formation of cobalt and nickel oxalate. In a mixture of cobalt and nickel, cobalt oxalate appeared to predominate precipitation and was dependent on the mixture ratios of the two metals. The presence of EPS together with oxalate in solution decreased the recovery of nickel but did not influence the recovery of cobalt. Concentrations of extracellular protein showed a significant decrease after precipitation while no significant difference was found for extracellular polysaccharide concentrations before and after oxalate precipitation. These results showed that extracellular protein rather than extracellular polysaccharide played a more important role in influencing the biorecovery of metal oxalates from solution. Excitation-emission matrix (EEM) fluorescence spectroscopy showed that aromatic protein-like and hydrophobic acid-like substances from the EPS complexed with cobalt but did not for nickel. The humic acid-like substances from the EPS showed a higher affinity for cobalt than for nickel.

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Section: Discussionmentioning

confidence: 58%

Biorecovery of cobalt and nickel using biomass-free culture supernatants from Aspergillus niger

Yang

Song

Ferrier

et al. 2019

Appl Microbiol Biotechnol

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“…The higher EPS may be indicative of microbial defense under stressed conditions promoting higher biofilm matrix. The formation of EPS has been found to play a significant role in Calcium carbonate precipitation in a number of previous studies (Bergdale et al, 2012; Ercole et al, 2012). …”

Section: Discussionmentioning

confidence: 91%

Bacterial Community Dynamics and Biocement Formation during Stimulation and Augmentation: Implications for Soil Consolidation

2017

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Microbially-induced CaCO3 precipitation (MICP) is a naturally occurring process wherein durable carbonates are formed as a result of microbial metabolic activities. In recent years, MICP technology has been widely harnessed for applications in civil engineering wherein synthesis of calcium carbonate crystals occurs at ambient temperature paving way for low energy biocement. MICP using pure urease (UA) and carbonic anhydrase (CA) producing bacteria has been promising in laboratory conditions. In the current study we enriched ureolytic and carbonic anhydrase communities in calcareous soil under biostimulation and bioaugmentation conditions and investigated the effect of microbial dynamics on carbonate precipitation, calcium carbonate polymorph selection and consolidation of biological sand column under nutrient limited and rich conditions. All treatments for stimulation and augmentation led to significant changes in the composition of indigenous bacterial population. Biostimulation as well as augmentation through the UA route was found to be faster and more effective compared to the CA route in terms of extracellular enzyme production and carbonate precipitation. Synergistic role of augmented cultures along with indigenous communities was recorded via both the routes of UA and CA as more effective calcification was seen in case of augmentation compared to stimulation. The survival of supplemented isolates in presence of indigenous bacterial communities was confirmed through sequencing of total diversity and it was seen that both UA and CA isolate had the potential to survive along with native communities under high nutrient conditions. Nutrient conditions played significant role in determining calcium carbonate polymorph fate as calcitic crystals dominated under high carbon supplementation. Finally, the consolidation of sand columns via stimulation and augmentation was successfully achieved through both UA and CA route under high nutrient conditions but higher consolidation in short time period was noticed in UA route. The study reports that based upon the organic carbon content in native soils, stimulation can be favored at sites with high organic carbon content while augmentation with repeated injections of nutrients can be applied on poor nutrient soils via different enrichment routes of microbial metabolism.

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“…Interestingly, the precipitation rate was also slightly but meaningfully enhanced by the negative-control cells, even though they did not have a detectable CO 2 hydration activity. This result is not surprising, because the E. coli cell surface carries a net negative charge (46), and this can accelerate the rate of CaCO 3 crystal growth, presenting another advantage of using a whole-cell system for CO 2 mineralization (26,47). These results collectively demonstrate that our design concept is useful in developing an efficient catalyst for accelerating CO 2 sequestration in CaCO 3 .…”

Section: Resultsmentioning

confidence: 59%

Engineered Escherichia coli with Periplasmic Carbonic Anhydrase as a Biocatalyst for CO₂Sequestration

Kim

Seo

et al. 2013

Appl Environ Microbiol

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Carbonic anhydrase is an enzyme that reversibly catalyzes the hydration of carbon dioxide (CO 2 ). It has been suggested recently that this remarkably fast enzyme can be used for sequestration of CO 2 , a major greenhouse gas, making this a promising alternative for chemical CO 2 mitigation. To promote the economical use of enzymes, we engineered the carbonic anhydrase from Neisseria gonorrhoeae (ngCA) in the periplasm of Escherichia coli, thereby creating a bacterial whole-cell catalyst. We then investigated the application of this system to CO 2 sequestration by mineral carbonation, a process with the potential to store large quantities of CO 2 . ngCA was highly expressed in the periplasm of E. coli in a soluble form, and the recombinant bacterial cell displayed the distinct ability to hydrate CO 2 compared with its cytoplasmic ngCA counterpart and previously reported wholecell CA systems. The expression of ngCA in the periplasm of E. coli greatly accelerated the rate of calcium carbonate (CaCO 3 ) formation and exerted a striking impact on the maximal amount of CaCO 3 produced under conditions of relatively low pH. It was also shown that the thermal stability of the periplasmic enzyme was significantly improved. These results demonstrate that the engineered bacterial cell with periplasmic ngCA can successfully serve as an efficient biocatalyst for CO 2 sequestration.

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Calcium Carbonate Mineralization: Involvement of Extracellular Polymeric Materials Isolated from Calcifying Bacteria

Cited by 60 publications

References 53 publications

Biorecovery of cobalt and nickel using biomass-free culture supernatants from Aspergillus niger

Biorecovery of cobalt and nickel using biomass-free culture supernatants from Aspergillus niger

Bacterial Community Dynamics and Biocement Formation during Stimulation and Augmentation: Implications for Soil Consolidation

Engineered Escherichia coli with Periplasmic Carbonic Anhydrase as a Biocatalyst for CO₂Sequestration

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Calcium Carbonate Mineralization: Involvement of Extracellular Polymeric Materials Isolated from Calcifying Bacteria

Cited by 60 publications

References 53 publications

Biorecovery of cobalt and nickel using biomass-free culture supernatants from Aspergillus niger

Biorecovery of cobalt and nickel using biomass-free culture supernatants from Aspergillus niger

Bacterial Community Dynamics and Biocement Formation during Stimulation and Augmentation: Implications for Soil Consolidation

Engineered Escherichia coli with Periplasmic Carbonic Anhydrase as a Biocatalyst for CO2Sequestration

Contact Info

Product

Resources

About

Engineered Escherichia coli with Periplasmic Carbonic Anhydrase as a Biocatalyst for CO₂Sequestration