Calcium deficiency in hydroxyapatite and its drug delivery applications

Koppala, Sivasankar; Swamiappan, Sasikumar; Gangarajula, Yuvaraj; Xu, Lei; Sadasivuni, Kishor Kumar; Ponnamma, Deepalekshmi; Vijayaraghavan, R.

doi:10.1049/mnl.2016.0675

Cited by 7 publications

(3 citation statements)

References 15 publications

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“…The increase in Ga content in the HAp samples is at the expense of reducing the Ca/P molar ratio. Thus, non-stoichiometric or Ca-deficient HAp (CDHAp) is obtained, which is more soluble than HAp [ 50 ]. If Ga does not enter the Ca site in HAp crystallites, it accumulates in the hydrated layer on the surface of crystallites.…”

Section: Discussionmentioning

confidence: 99%

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Gallium-Doped Hydroxyapatite Shows Antibacterial Activity against Pseudomonas aeruginosa without Affecting Cell Metabolic Activity

Mosina

Siverino

Stipniece

et al. 2023

JFB

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Calcium phosphates (CaPs) have been used in bone regeneration for decades. Among the described CaPs, synthetic hydroxyapatite (HAp) has a chemical composition similar to that of natural bone. Gallium-containing compounds have been studied since the 1970s for the treatment of autoimmune diseases and have shown beneficial properties, such as antibacterial activity and inhibition of osteoclast activity. In this study, we synthesized hydroxyapatite (HAp) powder with Ga doping ratios up to 6.9 ± 0.5 wt% using the wet chemical precipitation method. The obtained products were characterized using XRD, BET, FTIR, and ICP-MS. Ga3+ ion release was determined in the cell culture media for up to 30 days. Antibacterial activity was assessed against five bacterial species: Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, and Streptococcus pyogenes. The biocompatibility of the GaHAp samples was determined in human fibroblasts (hTERT-BJ1) through direct and indirect tests. The structure of the synthesized products was characteristic of HAp, as revealed with XRD and FTIR, although the addition of Ga caused a decrease in the crystallite size. Ga3+ was released from GaHAp paste in a steady manner, with approximately 40% being released within 21 days. GaHAp with the highest gallium contents, 5.5 ± 0.1 wt% and 6.9 ± 0.5 wt%, inhibited the growth of all five bacterial species, with the greatest activity being against Pseudomonas aeruginosa. Biocompatibility assays showed maintained cell viability (~80%) after seven days of indirect exposure to GaHAp. However, when GaHAp with Ga content above 3.3 ± 0.4 wt% was directly applied on the cells, a decrease in metabolic activity was observed on the seventh day. Overall, these results show that GaHAp with Ga content below 3.3 ± 0.4 wt% has attractive antimicrobial properties, without affecting the cell metabolic activity, creating a material that could be used for bone regeneration and prevention of infection.

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Section: Discussionmentioning

confidence: 99%

“…Interestingly, the HAp nanoparticles without Ga also showed delayed bacterial growth. This could be explained by the fact that nanoparticles themselves can have a negative effect on bacterial growth [ 48 , 50 ]. However, GaHAp did not show total inhibition of P. aeruginosa growth, as observed in the case of Ga(NO 3 ) 3 ·4.2H 2 O.…”

Section: Discussionmentioning

confidence: 99%

Gallium-Doped Hydroxyapatite Shows Antibacterial Activity against Pseudomonas aeruginosa without Affecting Cell Metabolic Activity

Mosina

Siverino

Stipniece

et al. 2023

JFB

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“…In this respect, biomimetic CDHA is more like an inorganic composition of natural bone, which also shows a lower Ca/P ratio than synthetic bone. Therefore, CDHA, which has a higher specific surface area than HA or tricalcium phosphate (TCP) [ 63 ], exhibits considerable promise for industrial production of synthetic bone substitutes [ 64 ], with potential applications extending to drug delivery systems. Its presence in the biomimetically deposited layer ensures good biocompatibility and bioactivity of oxidized NiTi.…”

Section: Discussionmentioning

confidence: 99%

Influence of Low Temperature Plasma Oxidizing on the Bioactivity of NiTi Shape Memory Alloy for Medical Applications

Witkowska,

Borowski,

Sowińska

et al. 2023

Materials

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The present study elucidates the impact of glow discharge oxidation within a low-temperature plasma environment on the bioactivity characteristics of an NiTi shape memory alloy. The properties of the produced surface layers, such as structure (TEM observations), surface morphology (SEM observations), chemical and phase composition (EDS and XRD measurements), wettability (optical gonimeter), and the biological response of osteoblasts and platelets to the oxidized surface compared with the NiTi alloy without a surface layer are presented. The presented surface modification of the NiTi shape memory alloy, achieved through oxidizing in a low-temperature plasma environment, led to the creation of a continuous surface layer composed of nanocrystalline titanium oxide TiO2 (rutile). The findings obtained from this study provide evidence that the oxidized layer augments the bioactivity of the shape memory alloy. This augmentation was substantiated through the spontaneous biomimetic deposition of apatite from a simulated body fluid (SBF) solution. Furthermore, the modified surface exhibited improved osteoblast proliferation, and enhanced platelet adhesion and activation. This proposed surface modification strategy holds promise as a prospective solution to enhance the biocompatibility and bioactivity of NiTi shape memory alloy intended for prolonged use in bone implant applications.

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Clinical Applications of Artificial Intelligence in Early and Accurate Detection of Low‐ConcentrationCVDBiomarkers

Laad¹,

Sajna²,

Sadasivuni³

et al. 2022

Predicting Heart Failure

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Calcium deficiency in hydroxyapatite and its drug delivery applications

Cited by 7 publications

References 15 publications

Gallium-Doped Hydroxyapatite Shows Antibacterial Activity against Pseudomonas aeruginosa without Affecting Cell Metabolic Activity

Gallium-Doped Hydroxyapatite Shows Antibacterial Activity against Pseudomonas aeruginosa without Affecting Cell Metabolic Activity

Influence of Low Temperature Plasma Oxidizing on the Bioactivity of NiTi Shape Memory Alloy for Medical Applications

Clinical Applications of Artificial Intelligence in Early and Accurate Detection of Low‐ConcentrationCVDBiomarkers

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