Calcium Delivery System Assembled by a Nanostructured Peptide Derived from the Sea Cucumber Ovum

Cui, Pengbo; Lin, Songyi; Han, Wei; Jiang, Pengfei; Zhu, Beiwei; Sun, Na

doi:10.1021/acs.jafc.9b04522

Cited by 43 publications

(38 citation statements)

References 45 publications

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“…The dissociation constant ( K d ) between DTDSEEEIR and iron was estimated to be 3.58 ± 1.26 × 10 –4 M –1 , and the association constant ( K a ) was 2.79 × 10 3 M at 25 °C. The similar value of K a was also observed for food protein-derived peptides like DHTKE (1.00 × 10 3 M), , NDEELNK (3.72 × 10 3 M), and EDLAALEK (8.59 × 10 3 M), which can serve as ligands for metal ions. This intermediate binding constant implied that iron might be released easily from the DTDSEEEIR–iron complex .…”

Section: Resultssupporting

confidence: 72%

Antarctic Krill Derived Nonapeptide as an Effective Iron-Binding Ligand for Facilitating Iron Absorption via the Small Intestine

Sun

Wang

et al. 2020

J. Agric. Food Chem.

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A novel nonapeptide DTDSEEEIR identified from Antarctic krill (Euphausia superba) iron-binding peptides was used in this study to analyze its iron-binding sites and structural changes after iron coordination. The enzymatic resistance and transport of DTDSEEEIR–iron during gastrointestinal digestion and absorption as well as the relationship between the DTDSEEEIR stability and the enhancement of iron absorption were further explored. Results revealed that iron ions spontaneously bound to the carboxyl, hydroxyl, and amino groups of the DTDSEEEIR peptide, which induced the folding of DTDSEEEIR to form a more orderly structure. The DTDSEEEIR peptide remained stable to a certain extent (79.60 ± 0.19%) after gastrointestinal digestion and the coordination of iron improved the digestive stability of the DTDSEEEIR peptide (93.89 ± 1.37%). Moreover, the stability of DTDSEEEIR across intestinal epithelium had a positive effect on iron absorption, which implied that DTDSEEEIR might carry iron ions through intestinal epithelial cells.

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Section: Resultssupporting

confidence: 72%

Antarctic Krill Derived Nonapeptide as an Effective Iron-Binding Ligand for Facilitating Iron Absorption via the Small Intestine

Sun

Wang

et al. 2020

J. Agric. Food Chem.

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“…The peptides with low molecular weight could favor calcium-binding peptides [26]. In addition, several peptides rich in acidic amino acids have been demonstrated to exert positive contributions for calcium chelation [49,50]. Values are given as the mean ± SD.…”

Section: Calcium-binding Capacity Analysismentioning

confidence: 99%

Elucidating the Calcium-Binding Site, Absorption Activities, and Thermal Stability of Egg White Peptide–Calcium Chelate

Bao

Zhang

Sun

et al. 2021

Foods

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With the current study, we aimed to determine the characteristics and calcium absorption capacity of egg white peptide–calcium complex (EWP-Ca) and determine the effect of sterilization on EWP-Ca to study the possibility of EWP-Ca as a new potential calcium supplement. The results of SEM and EDS showed a high calcium chelating ability between EWP and calcium, and the structure of EWP-Ca was clustered spherical particles due its combination with calcium. The FTIR and Raman spectrum results showed that EWP could chelate with calcium by carboxyl, phosphate, and amino groups, and peptide bonds may also participate in peptide–calcium binding. Moreover, the calcium absorption of EWP-Ca measured by the intestinal everted sac model in rats was 32.38 ± 6.83 μg/mL, significantly higher than the sample with CaCl2, and the mixture of EWP and Ca (p < 0.05) revealed appropriate calcium absorption capacity. The fluorescence spectra and CD spectra showed that sterilization caused a decrease in the content of α-helix and β-sheet and a significant increase in β-turn (p < 0.05). Sterilization changed the EWP-Ca structure and decreased its stability; the calcium-binding capacity of EWP-Ca after sterilization was decreased to 41.19% (p < 0.05). Overall, these findings showed that EWP could bind with calcium, form a peptide–calcium chelate, and serve as novel carriers for calcium supplements.

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“…A peptide (EDLAALEK) derived from sea cucumber eggs was shown to have a superior CaBC. FTIR spectra showed a shift from 3432 cm −1 to 3419 cm −1 for the corresponding N-H vibrations, indicating that the amino nitrogen atom may have acted as a calcium binding site [ 73 ]. Wu et al found that new bands with wave numbers of 555.07 cm −1 appeared following the binding of the peptide to calcium as a result of the stretching vibrations of N-Ca [ 13 ].…”

Section: Calcium Binding Sites On Peptidesmentioning

confidence: 99%

The Formation, Structural Characteristics, Absorption Pathways and Bioavailability of Calcium–Peptide Chelates

Zhang

Ying

et al. 2022

Foods

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Calcium is one of the most important mineral elements in the human body and is closely related to the maintenance of human health. To prevent calcium deficiency, various calcium supplements have been developed, but their application tends to be limited by low calcium content and highly irritating effects on the stomach, among other side effects. Recently, calcium–peptide chelates, which have excellent stability and are easily absorbed, have received attention as an alternative emerging calcium supplement. Calcium-binding peptides (CaBP) are usually obtained via the hydrolysis of animal or plant proteins, and calcium-binding capacity (CaBC) can be further improved through chromatographic purification techniques. In calcium ions, the phosphate group, carboxylic group and nitrogen atom in the peptide are the main binding sites, and the four modes of combination are the unidentate mode, bidentate mode, bridging mode and α mode. The stability and safety of calcium–peptide chelates are discussed in this paper, the intestinal absorption pathways of calcium elements and peptides are described, and the bioavailability of calcium–peptide chelates, both in vitro and in vivo, is also introduced. This review of the research status of calcium–peptide chelates aims to provide a reasonable theoretical basis for their application as calcium supplementation products.

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Calcium Delivery System Assembled by a Nanostructured Peptide Derived from the Sea Cucumber Ovum

Cited by 43 publications

References 45 publications

Antarctic Krill Derived Nonapeptide as an Effective Iron-Binding Ligand for Facilitating Iron Absorption via the Small Intestine

Antarctic Krill Derived Nonapeptide as an Effective Iron-Binding Ligand for Facilitating Iron Absorption via the Small Intestine

Elucidating the Calcium-Binding Site, Absorption Activities, and Thermal Stability of Egg White Peptide–Calcium Chelate

The Formation, Structural Characteristics, Absorption Pathways and Bioavailability of Calcium–Peptide Chelates

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