Calcium influx differentially regulates migration velocity and directedness in response to electric field application

Babona-Pilipos, Robart; Liu, N.; Pritchard-Oh, Alex; Mok, Alexander; Badawi, D.; Popović, Miloš R.; Morshead, Cindi M.

doi:10.1016/j.yexcr.2018.04.031

Cited by 28 publications

(18 citation statements)

References 76 publications

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“…In many cases, the activity of other types of ion channels contributes to cancer cell migration through their indirect effect on Ca 2+ i. Calcium is important for cell adhesion turnover and a polarized Ca 2+ i concentration gradient of higher Ca 2+ concentration in the leading edge of the cell and vice versa for the trailing edge is found in many migrating cell types. [66][67][68] The protease calpain is dependent on Ca 2+ and will inactivate E-cadherin to facilitate the local disassembly of focal adhesions to allow detachment of the rear part of the cell, facilitating migration. 69 Calcium is also involved in promoting EMT pathways and the activity of matrix metalloproteinases (MMPs) to drive ECM degradation and cell invasion.…”

Section: Ion Channelsmentioning

confidence: 99%

“…156 One of the key early events in normal cell electrotaxis is a polarized increase of intracellular Ca 2+ ; when Ca 2+ influx is inhibited, cells can no longer detect and respond to an EF, for example, when VGCCs are blocked with Ni 2+ or Sr. 2+154,157,158 Polarization of Ca 2+ within a cell asymmetrically activates several downstream pathways involved in migration such as receptor tyrosine kinases, PI3K, Rho GTPases, and ERK, which then elicit cytoskeletal changes necessary for directed migration. [66][67][68] The mechanisms known to drive electrotaxis in normal cell types appear to also be involved in electrotactic responses of cancer cells, although the regulation of these mechanisms may differ. Similar to the effects of ion flux, these mechanisms include changes in Ca 2+ concentration leading to actin polymerization/depolymerization and cell adhesion, and growth factor signaling (Fig.…”

Section: Local Changes In Efsmentioning

confidence: 99%

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Bioelectric Control of Metastasis in Solid Tumors

2019

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As the leading cause of death in cancer, there is an urgent need to develop treatments to target the dissemination of primary tumor cells to secondary organs, known as metastasis. Bioelectric signaling has emerged in the last century as an important controller of cell growth, and with the development of current molecular tools we are now beginning to identify its role in driving cell migration and metastasis in a variety of cancer types. This review summarizes the currently available research for bioelectric signaling in solid tumor metastasis. We review the steps of metastasis and discuss how these can be controlled by bioelectric cues at the level of a cell, a population of cells, and the tissue. The role of ion channel, pump, and exchanger activity and ion flux is discussed, along with the importance of the membrane potential and the relationship between ion flux and membrane potential. We also provide an overview of the evidence for control of metastasis by external electric fields (EFs) and draw from examples in embryogenesis and regeneration to discuss the implications for endogenous EFs. By increasing our understanding of the dynamic properties of bioelectric signaling, we can develop new strategies that target metastasis to be translated into the clinic.

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Section: Ion Channelsmentioning

confidence: 99%

Section: Local Changes In Efsmentioning

confidence: 99%

Bioelectric Control of Metastasis in Solid Tumors

2019

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“…In addition to signal pathways, ion channels such as voltage-gated Ca 2+ channels is a vital part of membrane polarization and cell response during ES [118]. All living cells have a transmembrane potential.…”

Section: Introductionmentioning

confidence: 99%

Electrical stimulation as a novel tool for regulating cell behavior in tissue engineering

et al. 2019

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Recently, electrical stimulation as a physical stimulus draws lots of attention. It shows great potential in disease treatment, wound healing, and mechanism study because of significant experimental performance. Electrical stimulation can activate many intracellular signaling pathways, and influence intracellular microenvironment, as a result, affect cell migration, cell proliferation, and cell differentiation. Electrical stimulation is using in tissue engineering as a novel type of tool in regeneration medicine. Besides, with the advantages of biocompatible conductive materials coming into view, the combination of electrical stimulation with suitable tissue engineered scaffolds can well combine the benefits of both and is ideal for the field of regenerative medicine. In this review, we summarize the various materials and latest technologies to deliver electrical stimulation. The influences of electrical stimulation on cell alignment, migration and its underlying mechanisms are discussed. Then the effect of electrical stimulation on cell proliferation and differentiation are also discussed.

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“…The application of a higher electric field gradient against the natural RMS gradient redirects the migration of grafted NPCs (Feng et al, 2017;Iwasa et al, 2019). These galvanotactic effects seem to be mediated by P2Y1 purinergic receptors and are accompanied by Ca 2+ fluctuations (Cao et al, 2013;Babona-Pilipos et al, 2018). The downregulation of P2Y1 receptors in neuroblasts results in a loss of directionality of migration in response to an electric field (Cao et al, 2013).…”

Section: Galvonotaxismentioning

confidence: 99%

Intrinsic Mechanisms Regulating Neuronal Migration in the Postnatal Brain

Bressan

Saghatelyan

2021

Front. Cell. Neurosci.

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Neuronal migration is a fundamental brain development process that allows cells to move from their birthplaces to their sites of integration. Although neuronal migration largely ceases during embryonic and early postnatal development, neuroblasts continue to be produced and to migrate to a few regions of the adult brain such as the dentate gyrus and the subventricular zone (SVZ). In the SVZ, a large number of neuroblasts migrate into the olfactory bulb (OB) along the rostral migratory stream (RMS). Neuroblasts migrate in chains in a tightly organized micro-environment composed of astrocytes that ensheath the chains of neuroblasts and regulate their migration; the blood vessels that are used by neuroblasts as a physical scaffold and a source of molecular factors; and axons that modulate neuronal migration. In addition to diverse sets of extrinsic micro-environmental cues, long-distance neuronal migration involves a number of intrinsic mechanisms, including membrane and cytoskeleton remodeling, Ca2+ signaling, mitochondria dynamics, energy consumption, and autophagy. All these mechanisms are required to cope with the different micro-environment signals and maintain cellular homeostasis in order to sustain the proper dynamics of migrating neuroblasts and their faithful arrival in the target regions. Neuroblasts in the postnatal brain not only migrate into the OB but may also deviate from their normal path to migrate to a site of injury induced by a stroke or by certain neurodegenerative disorders. In this review, we will focus on the intrinsic mechanisms that regulate long-distance neuroblast migration in the adult brain and on how these pathways may be modulated to control the recruitment of neuroblasts to damaged/diseased brain areas.

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Calcium influx differentially regulates migration velocity and directedness in response to electric field application

Cited by 28 publications

References 76 publications

Bioelectric Control of Metastasis in Solid Tumors

Bioelectric Control of Metastasis in Solid Tumors

Electrical stimulation as a novel tool for regulating cell behavior in tissue engineering

Intrinsic Mechanisms Regulating Neuronal Migration in the Postnatal Brain

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