Calcium phosphate coupled Newcastle disease vaccine elicits humoral and cell mediated immune responses in chickens

Koppad, Sanganagouda; Raj, G. Dhinakar; Gopinath, V.P.; Kirubaharan, J. John; Thangavelu, A.; Thiagarajan, V.

doi:10.1016/j.rvsc.2010.09.008

Cited by 21 publications

(6 citation statements)

References 27 publications

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“…CA-NP30 may enhance the humoral and cellular immune response in mice. Sanganagouda Koppad et al [16] coupled calcium phosphate (CaP) particles with inactivated Newcastle disease virus (NDV) vaccines. Compared to commercial live vaccines (RDV'F '), CaP-conjugated NDV vaccines can also induce long-term hematocrit inhibition (HI) and enzyme-linked immunosorbent assay (ELISA) titers, (CMI) from 2 weeks to 4 weeks after inoculation.…”

Section: Journal Of Infectious Diseases and Therapymentioning

confidence: 99%

Construction and Immunological Evaluation of Immunostimulatory Nanocomposites with Vaccine Potential

Zhong¹,

Zhuang²

2017

J Infect Dis Ther

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For a long time, the most important means of controlling infectious diseases is prevention, and vaccination is considered the most effective measures, so the preparation and improvement of the vaccine is not only supported by the medical profession, it is the concern of the broad masses of the people. A number of biomolecules have been used as antigens to construct novel vaccine preparations, including Virus-like particles (VLPs) or bacteria, dendritic cells, nucleic acids and peptides. Immunostimulatory nanocomposites are based on the above molecules as vectors, encapsulating immune adjuvants, and nanoparticles for the construction of novel vaccines. Compared to traditional vaccines, adjuvants, nanoparticles alone, the efficacy of immunostimulatory nanocomposites as potential vaccines shows increased CD4 + , CD8+ T cell count, secretion of cytokine interferon-gamma and IL-4 functions also significantly enhanced, in addition, the use of nanoparticles enhances the Immune irritation of adjuvants, VLPs and other molecules on humoral and cellular immune responses. Thus, immunostimulatory nanocomposites may be potential vaccines for future prevention and treatment applications.

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Section: Journal Of Infectious Diseases and Therapymentioning

confidence: 99%

Construction and Immunological Evaluation of Immunostimulatory Nanocomposites with Vaccine Potential

Zhong¹,

Zhuang²

2017

J Infect Dis Ther

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“…The end point titre was expressed as 50% embryo lethal dose (ELD 50 ) per ml as calculated by the method described by Reed and Muench (1938). Infective allantoic fluid (AF) containing field isolate of NDV having ELD 50 10 8.7 /1 ml of AF was inactivated by treating with formalin at final concentration of 1:1000 following the method described by Koppad et al (2011). Inactivation of NDV was checked by two serial passages in ECE resulting in live embryos with no HA activity in their allantoic fluids.…”

Section: Titration and Inactivation Of The Field Isolate Of Ndvmentioning

confidence: 99%

Biological characterization and determination of comparative efficacy of an inactivated Newcastle disease virus vaccine prepared from velogenic strain

Hossain¹,

Azad²,

Akter³

et al. 2014

Afr. J. Microbiol. Res.

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“…Vaccination is an important measure used successfully for prevention and control of Newcastle disease for longer. Koppad et al [13] stated that inactivated Newcastle disease viral particles coupled with calcium phosphate nanoparticle as a adjuvant exhibited prolonged and improved humoral and cell mediated immune response in comparison with commercial live ND vaccines. Robin et al [14] constructed recombinant HTV clones, which expressed the fusion and HN proteins of NDV and when chickens were inoculated at 1 day age with recombinant HVT expressing the NDV fusion protein, had an immunological response and were protected (>90%) against lethal intramuscular challenge at 28 days of age with the neurotropic velogenic NDV strain Texas GB.…”

Section: Expression Of Interferon Gamma (Ifn-γ)mentioning

confidence: 99%

Determination of Immune Potentials of Recombinant Fusion and Recombinant Haemagglutinin-Neuraminidase Antigens of Newcastle Disease Virus (NDV)

K¹

2014

J Veterinar Sci Technol

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Chicken are the most abundant birds in the world with a population of more than 24 billion. They are mainly kept for egg and meat production. Important economic lossess for the industry can occur, due to viral diseases such as Avian influenza and Newcastle disease. Vaccination is the only available tool to curb the outbreak of epidemics in poultry. Till today only few good vaccines are available to prevent the epidemic diseases. Recombinant DNA technology led to the development of recombinant vaccines and subunit vaccines but immunopotency of recombinant vaccines and subunit vaccines are not well understood in Newcastle disease vaccines. Hence, in this study, we cloned and expressed the fusion and haemagglutinin-neuraminidase genes of NDV in insect cells. Immunological study was carried out to assess the immunopotency of rec-F and rec-HN antigens in commercial broilers. Humoral and cell mediated immune response were evaluated. The immunogenic potentials of recombinant antigens along with commercial live and inactivated vaccine in stimulating humoral antibody response was recorded by haemagglutination inhibition (HI) test and ELISA. Cell mediated immune responses in stimulating the peripheral blood mononuclear cells by recombinant antigens were measured using tetrazolium dye (MTT assay). Expression of cytokines such as interferon alfa and interferon gamma, stimulated by recombinant F and HN antigens and commercial vaccines were studied by Real-time polymerase chain reaction. Our results revealed that recombinant antigens had similar immuneresponse as commercial incativated vaccines but less efficient in protecing the immunised birds than commercial live vaccine. Fusion protein has shown better protection than HN protein and combination of both antigens revealed more potency than individual antigen.

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Calcium phosphate coupled Newcastle disease vaccine elicits humoral and cell mediated immune responses in chickens

Cited by 21 publications

References 27 publications

Construction and Immunological Evaluation of Immunostimulatory Nanocomposites with Vaccine Potential

Construction and Immunological Evaluation of Immunostimulatory Nanocomposites with Vaccine Potential

Biological characterization and determination of comparative efficacy of an inactivated Newcastle disease virus vaccine prepared from velogenic strain

Determination of Immune Potentials of Recombinant Fusion and Recombinant Haemagglutinin-Neuraminidase Antigens of Newcastle Disease Virus (NDV)

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