Calcium Phosphate Crystallization on Electrospun Cellulose Non‐Woven Fabrics Containing Synthetic Phosphorylated Polypeptides

Hayashi, Shinya; Ohkawa, Kousaku; Yamamoto, Hiroyuki; Yamaguchi, M.; Kimoto, Shigenari; Kurata, Shigeaki; Shinji, Hisaaki

doi:10.1002/mame.200800335

Cited by 9 publications

(11 citation statements)

References 29 publications

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“…13 C NMR and DEPT spectra were recorded on Bruker Spectrometer (50, 100, or 125 MHz), and reported relative signals according to the deuterated solvent used. 31 P NMR spectra recorded on Bruker Spectrometer (202.46 MHz) using an internal 85 % aqueous H 3 PO 4 as reference. FT-IR spectra were recorded on a Perkin-Elmer FT-IR spectrum GX instrument by making KBr pellets.…”

Section: Methodsmentioning

confidence: 99%

“…The use of such phosphorylation and de‐phosphorylation reactions have triggered the disintegration and modulation of polymeric assemblies and nanoparticles . In addition to that, phosphonate, in comparison to phosphate, may regulate mineral deposition differently during biomineralization due to its somewhat differing geometry . Taken together, direct carbon‐phosphorous linkage in phosphono polypeptides may limit their ability to mimic natural phosphoproteins .…”

Section: Introductionmentioning

confidence: 99%

“…[25][26][27][28][29][30] In addition to that, phosphonate, in comparison to phosphate, may regulate mineral deposition differently during biomineralization due to its somewhat differing geometry. [31][32][33][34][35][36][37] Taken together, direct carbonphosphorous linkage in phosphono polypeptides may limit their ability to mimic natural phosphoproteins. [15][16][17][18][19] Recently, Lu et al reported a controlled synthesis of poly(L-phosphotyrosine) that is structurally identical to its natural analog and found it to be responsive towards the enzyme alkaline phosphatase (ALP).…”

Section: Introductionmentioning

confidence: 99%

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Synthesis of Phospho‐Polypeptides via Phosphate‐Containing N‐Carboxyanhydride: Application in Enzyme‐Induced Self‐Assembly, and Calcium Carbonate Mineralization

et al. 2020

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An easy synthetic strategy was developed to synthesize the phosphate-functionalized amino acid N-carboxyanhydride (NCA), using simple primary amine initiators to obtain homo and block phospho-polypeptides with controlled molecular weight and molecular weight distribution. The methodology was extended to the synthesis of the end-functionalized homo polypeptides (15 to 50 repeat unit) and block co-polypeptides with PEG (0.7 K, 2 K, and 5 K) and glycopolypeptide (15-unit mannose glycopolypeptide) as one of the blocks. The deprotected fully water-soluble anionic phosphate-based polypeptides showed pH-dependent helical conformation with a helical content of 20 %, which further changed to β-sheets upon addition of the enzyme alkaline phosphatase (ALP) due to dephosphorylation. The block co-polypeptide containing PEG as one of the blocks led to its self-assembly into colloidal structures, such as vesicles with a hydrodynamic diameter of 250 nm, due to the formation of amphiphilic block co-polymer upon dephosphorylation. The nature of the colloidal structures formed can be temporally controlled by the extent of dephosphorylation. Finally, the phospho-polypeptides serve as a template for the mineralization of calcium carbonate with varying polymorphs and morphologies.[a] B. Mondal, S. Panda, T. Dutta, Dr.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Synthesis of Phospho‐Polypeptides via Phosphate‐Containing N‐Carboxyanhydride: Application in Enzyme‐Induced Self‐Assembly, and Calcium Carbonate Mineralization

et al. 2020

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“…Figure 4 is an example study for this approach, and the cellulose-ESNW subcomponent is a synthetic polypeptide, copoly[Ser(PO 3 H 2 ) x , Asp y ] ( x : y = 100:0, 75:25, 50:50, 25:75), on which serine residues are fully phosphorylated. The weight ratio of copoly[Ser(PO 3 H 2 ) x , Asp y ] towards cellulose was 1.0–1.2 wt % in a TFA solution, and then after the ES process, the products, cellulose-copoly[Ser(PO 3 H 2 ) x , Asp y ]-composite-ESNW, was observed using a scanning electron microscope (Figure 4) [47]. The chemical structure of copoly[Ser(PO 3 H 2 ) x , Asp y ] was designed on the basis of osteogenic proteins, of which their role is to induce the hydroxyapatite deposition to make hard tissues, teeth and bone.…”

Section: Applied Research Studies For the Functionalization Of Celmentioning

confidence: 99%

Nanofibers of Cellulose and Its Derivatives Fabricated Using Direct Electrospinning

Ohkawa

2015

Molecules

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A short review with 49 references describes the electrospinninng (ES) process for polysaccharides, cellulose and chitosan, and their derivatives, including cellulose acetate and hydroxypropyl cellulose. A majority of applied studies adopted a two step-process, in which the cellulose acetate was used for the first ES process, followed by acetyl group removal to regenerate cellulose thin fibers. The electrospun nonwoven fabrics (ESNW) of regenerated cellulose can be modified by introduction of aldehyde groups by oxidative cleavage of vicinal diols using periodates, and these aldehyde groups serve as acceptors of foreign substances, with various chemical/biological functions, to be immobilized on the fiber surfaces in the ESNW matrices. Direct electrospinning of cellulose from trifluroacetic acid solution was also developed and the applied studies were summarized to conclude the current trends of interests in the ES and related technologies.

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“…We then continued a series of applied research studies using the composite ESNWs which were prepared from polysaccharides–synthetic phosphorylated poly(amino acid)s for crystallization of the calcium phosphate in an aqueous environment. 6,7 More recently, we have reported the immobilization of an enzyme on the chemically modified hydroxypropyl cellulose ESNWs. 8…”

mentioning

confidence: 99%

Cationic derivative of electrospun non-woven cellulose-chitosan composite fabrics for immobilization of aminoacylase-I

Devarayan

Miyamoto

Hachisu

et al. 2013

Textile Research Journal

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The chemical modification of cellulose (Cs)-chitosan (Ch)-electrospun non-woven (ESNW) composite fabrics is described. Since the as-spun Cs/Ch-composite ESNW (Cs : Ch ¼ 4 : 6) deforms in water, an insolublilization procedure using an alkaline-ethanol solution was developed to extend the applications in an aqueous environment. The fine fiber surface was modified with bifunctional isocyanate in order to introduce the -NCO groups on the ESNW surfaces. The -NCO groups were condensed with N,N-(diethyl)ethylene diamine to generate a cationic diethylaminoethyl (DEAE)-ESNW. An enzyme, aminoacylase-I, was immobilized onto the cationic matrix under mild conditions. The immobilized enzyme was subjected to the stereo-specific recognition of N -acetyl-methionines. The results suggest that the chemically modified Cs/Ch-ESNW is a useful support matrix for biological catalysts.Electrospinning is a unique technique for the fabrication of polymer solutions into fine fibers, which are referred to as electrospun non-woven fabrics (ESNWs). The electrospun fibers often have average diameters ranging from submicron to nano-scale. 1,2 Our research group has reported the preparation of chitosan (Ch) 3,4 and cellulose (Cs) 5 nanofibers via a conventional electrospinning method for the first time. We then continued a series of applied research studies using the composite ESNWs which were prepared from polysaccharides-synthetic phosphorylated poly(amino acid)s for crystallization of the calcium phosphate in an aqueous environment. 6,7 More recently, we have reported the immobilization of an enzyme on the chemically modified hydroxypropyl cellulose ESNWs. 8 Cellulose and chitosan (Scheme 1) are two widely available biomaterials, which have undergone more research studies. 9 Our recent report on the direct electrospinning of cellulose-chitosan composite ESNWs is promising for the production of composite fibers with average nano-scale diameters. 10 The as-spun Cs/Ch-ESNW in water tends to swell or become distorted into a thin film due to the presence of trifluoroacetic acid (TFA) salts, which were derived from the spinning solvent. An appropriate post-spun treatment

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Calcium Phosphate Crystallization on Electrospun Cellulose Non‐Woven Fabrics Containing Synthetic Phosphorylated Polypeptides

Cited by 9 publications

References 29 publications

Synthesis of Phospho‐Polypeptides via Phosphate‐Containing N‐Carboxyanhydride: Application in Enzyme‐Induced Self‐Assembly, and Calcium Carbonate Mineralization

Synthesis of Phospho‐Polypeptides via Phosphate‐Containing N‐Carboxyanhydride: Application in Enzyme‐Induced Self‐Assembly, and Calcium Carbonate Mineralization

Nanofibers of Cellulose and Its Derivatives Fabricated Using Direct Electrospinning

Cationic derivative of electrospun non-woven cellulose-chitosan composite fabrics for immobilization of aminoacylase-I

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